PMID- 35585076
OWN - NLM
STAT- MEDLINE
DCOM- 20220520
LR  - 20220716
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 May 18
TI  - Antimicrobial potential and osteoblastic cell growth on electrochemically 
      modified titanium surfaces with nanotubes and selenium or silver incorporation.
PG  - 8298
LID - 10.1038/s41598-022-11804-6 [doi]
LID - 8298
AB  - Titanium nanotube surfaces containing silver, zinc, and copper have shown 
      antimicrobial effects without decreasing osteoblastic cell growth. In this 
      in-vitro study we present first results on the biological evaluation of surface 
      modifications by incorporating selenium and silver compounds into 
      titanium-dioxide (TiO(2)) nanotubes by electrochemical deposition. 
      TiO(2)-nanotubes (TNT) and Phosphate-doped TNT (pTNT) were grown on the surface 
      of Ti6Al4V discs by anodization. Hydroxyapatite (HA), selenium (Se) and silver 
      (Ag) compounds were incorporated by electrochemical deposition. Colony forming 
      units of Staphylococcus epidermidis (DSM 3269) were significantly decreased in 
      SepTNT (0.97 +/- 0.18 x 10(6) CFU/mL), SepTNT-HA (1.2 +/- 0.39 x 10(6) CFU/mL), 
      AgpTNT (1.36 +/- 0.42 x 10(6) CFU/mL) and Ag(2)SepTNT (0.999 +/- 0.12 x 10(6) CFU/mL) 
      compared to the non-modified control (2.2 +/- 0.21 x 10(6) CFU/mL). Bacterial 
      adhesion was calculated by measuring the covered area after fluorescence 
      staining. Adhesion was lower in SepTNT (37.93 +/- 12%; P = 0.004), pTNT 
      (47.3 +/- 6.3%, P = 0.04), AgpTNT (24.9 +/- 1.8%; P < 0.001) and Ag(2)SepTNT 
      (14.9 +/- 4.9%; P < 0.001) compared to the non-modified control (73.7 +/- 11%). 
      Biofilm formation and the growth of osteoblastic cells (MG-63) was observed by 
      using Crystal Violet staining. Biofilm formation was reduced in SepTNT (22 +/- 3%, 
      P = 0.02) and Ag(2)SepTNT discs (23 +/- 11%, P = 0.02) compared to the non-modified 
      control (54 +/- 8%). In comparison with the non-modified control the modified 
      SepTNT-HA and pTNT surfaces showed a significant higher covered area with 
      osteoblastic MG-63-cells. Scanning electron microscope (SEM) images confirmed 
      findings regarding bacterial and osteoblastic cell growth. These findings show a 
      potential synergistic effect by combining selenium and silver with titanium 
      nanotubes.
CI  - (c) 2022. The Author(s).
FAU - Staats, Kevin
AU  - Staats K
AD  - Department of Orthopedics and Trauma Surgery, Medical University of Vienna, 
      Waehringer Guertel 18-20, 1090, Vienna, Austria. kevin.staats@meduniwien.ac.at.
FAU - Pilz, Magdalena
AU  - Pilz M
AD  - Department of Orthopedics and Trauma Surgery, Medical University of Vienna, 
      Waehringer Guertel 18-20, 1090, Vienna, Austria.
FAU - Sun, Jie
AU  - Sun J
AD  - Competence Center of Electrochemical Surface Technology (CEST GmbH), Wiener 
      Neustadt, Austria.
FAU - Boiadjieva-Scherzer, Tzvetanka
AU  - Boiadjieva-Scherzer T
AD  - Competence Center of Electrochemical Surface Technology (CEST GmbH), Wiener 
      Neustadt, Austria.
FAU - Kronberger, Hermann
AU  - Kronberger H
AD  - Institute for Chemical Technology and Analytics, Technical University of Vienna, 
      Vienna, Austria.
FAU - Tobudic, Selma
AU  - Tobudic S
AD  - Department of Internal Medicine I, Division of Infectious Diseases and Tropical 
      Medicine, Medical University of Vienna, Vienna, Austria.
FAU - Windhager, Reinhard
AU  - Windhager R
AD  - Department of Orthopedics and Trauma Surgery, Medical University of Vienna, 
      Waehringer Guertel 18-20, 1090, Vienna, Austria.
FAU - Holinka, Johannes
AU  - Holinka J
AD  - Department of Orthopedics and Trauma Surgery, Medical University of Vienna, 
      Waehringer Guertel 18-20, 1090, Vienna, Austria.
LA  - eng
PT  - Journal Article
DEP - 20220518
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Anti-Bacterial Agents)
RN  - 3M4G523W1G (Silver)
RN  - 91D9GV0Z28 (Durapatite)
RN  - D1JT611TNE (Titanium)
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Anti-Bacterial Agents/chemistry/pharmacology
MH  - Durapatite/chemistry
MH  - *Nanotubes/chemistry
MH  - *Selenium/pharmacology
MH  - Silver/chemistry/pharmacology
MH  - Surface Properties
MH  - Titanium/chemistry/pharmacology
PMC - PMC9117198
COIS- The authors declare no competing interests.
EDAT- 2022/05/19 06:00
MHDA- 2022/05/21 06:00
PMCR- 2022/05/18
CRDT- 2022/05/18 23:16
PHST- 2022/01/24 00:00 [received]
PHST- 2022/04/05 00:00 [accepted]
PHST- 2022/05/18 23:16 [entrez]
PHST- 2022/05/19 06:00 [pubmed]
PHST- 2022/05/21 06:00 [medline]
PHST- 2022/05/18 00:00 [pmc-release]
AID - 10.1038/s41598-022-11804-6 [pii]
AID - 11804 [pii]
AID - 10.1038/s41598-022-11804-6 [doi]
PST - epublish
SO  - Sci Rep. 2022 May 18;12(1):8298. doi: 10.1038/s41598-022-11804-6.