PMID- 35585097
OWN - NLM
STAT- MEDLINE
DCOM- 20220520
LR  - 20220716
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 May 18
TI  - Cytogenetic testing by fluorescence in situ hybridization is improved by plasma 
      cell sorting in multiple myeloma.
PG  - 8287
LID - 10.1038/s41598-022-11676-w [doi]
LID - 8287
AB  - Accurate detection of cytogenetic abnormalities has become more important for 
      improving risk-adapted treatment strategies in multiple myeloma (MM). However, 
      precise cytogenetic testing by fluorescence in situ hybridization (FISH) is 
      challenged by the dilution effect of bone marrow specimens and poor growth of 
      plasma cells ex vivo. It has been suggested that FISH should be performed in 
      combination with plasma cell enrichment strategies. We examined cytogenetic 
      abnormalities in newly diagnosed MM and compared the efficacy of three different 
      enrichment modalities for FISH: direct FISH (n = 137), fluorescence 
      immunophenotyping and interphase cytogenetics as a tool for the investigation of 
      neoplasms (FICTION) technique (n = 224), and a plasma cell sorting FISH with 
      fluorescence-activated cell sorter (FACS) (n = 132). FISH disclosed cytogenetic 
      abnormalities in 38.0% of samples by direct FISH, 56.3% by FICTION, and 95.5% by 
      FACS-FISH, and the percentage of cells with abnormal signals detected by FISH was 
      significantly higher by FACS-FISH than direct FISH or FICTION. Our results 
      suggest that the efficacy of FISH is dependent on the plasma cell enrichment 
      modalities and reveal that plasma cell sorting FISH with FACS enables better 
      detection of cytogenetic abnormalities in diagnostic MM samples.
CI  - (c) 2022. The Author(s).
FAU - Ha, Jihye
AU  - Ha J
AD  - Department of Laboratory Medicine, Hanyang University College of Medicine, Seoul, 
      Republic of Korea.
FAU - Cho, Hyunsoo
AU  - Cho H
AD  - Department of Internal Medicine, Yonsei University College of Medicine, Severance 
      Hospital, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
FAU - Lee, Taek Gyu
AU  - Lee TG
AD  - Graduate School of Medical Science, Brain Korea 21 PLUS Project, Yonsei 
      University College of Medicine, Seoul, Republic of Korea.
FAU - Shin, Saeam
AU  - Shin S
AD  - Department of Laboratory Medicine, Yonsei University College of Medicine, 
      Severance Hospital, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea. 
      SAEAM0304@yuhs.ac.
FAU - Chung, Haerim
AU  - Chung H
AD  - Department of Internal Medicine, Yonsei University College of Medicine, Severance 
      Hospital, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
FAU - Jang, Ji Eun
AU  - Jang JE
AD  - Department of Internal Medicine, Yonsei University College of Medicine, Severance 
      Hospital, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
FAU - Kim, Soo-Jeong
AU  - Kim SJ
AD  - Department of Internal Medicine, Yonsei University College of Medicine, Severance 
      Hospital, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
FAU - Cheong, June-Won
AU  - Cheong JW
AD  - Department of Internal Medicine, Yonsei University College of Medicine, Severance 
      Hospital, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
FAU - Lee, Seung-Tae
AU  - Lee ST
AD  - Department of Laboratory Medicine, Yonsei University College of Medicine, 
      Severance Hospital, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
AD  - Dxome Co. Ltd. 8, Seongnam-daero 331beon-gil, Bundang-gu, Seongnam-si,, 
      Gyeonggi-do, Republic of Korea.
FAU - Kim, Jin Seok
AU  - Kim JS
AD  - Department of Internal Medicine, Yonsei University College of Medicine, Severance 
      Hospital, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea. 
      hemakim@yuhs.ac.
FAU - Choi, Jong Rak
AU  - Choi JR
AD  - Department of Laboratory Medicine, Yonsei University College of Medicine, 
      Severance Hospital, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
AD  - Dxome Co. Ltd. 8, Seongnam-daero 331beon-gil, Bundang-gu, Seongnam-si,, 
      Gyeonggi-do, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220518
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Chromosome Aberrations
MH  - Cytogenetic Analysis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Multiple Myeloma/diagnosis/genetics
MH  - *Plasma Cells
PMC - PMC9117238
COIS- The authors declare no competing interests.
EDAT- 2022/05/19 06:00
MHDA- 2022/05/21 06:00
PMCR- 2022/05/18
CRDT- 2022/05/18 23:18
PHST- 2021/12/09 00:00 [received]
PHST- 2022/04/28 00:00 [accepted]
PHST- 2022/05/18 23:18 [entrez]
PHST- 2022/05/19 06:00 [pubmed]
PHST- 2022/05/21 06:00 [medline]
PHST- 2022/05/18 00:00 [pmc-release]
AID - 10.1038/s41598-022-11676-w [pii]
AID - 11676 [pii]
AID - 10.1038/s41598-022-11676-w [doi]
PST - epublish
SO  - Sci Rep. 2022 May 18;12(1):8287. doi: 10.1038/s41598-022-11676-w.