PMID- 35587044
OWN - NLM
STAT- MEDLINE
DCOM- 20220805
LR  - 20221209
IS  - 2192-2659 (Electronic)
IS  - 2192-2640 (Linking)
VI  - 11
IP  - 15
DP  - 2022 Aug
TI  - Magnesium Oxide/Poly(l-lactide-co-epsilon-caprolactone) Scaffolds Loaded with Neural 
      Morphogens Promote Spinal Cord Repair through Targeting the Calcium Influx and 
      Neuronal Differentiation of Neural Stem Cells.
PG  - e2200386
LID - 10.1002/adhm.202200386 [doi]
AB  - Because of the limited regenerative ability of the central nervous system (CNS), 
      effective treatments for spinal cord injury (SCI) are still lacking. After SCI, 
      neuron loss and axon regeneration failure often result in irreversible functional 
      impairment. The calcium overload induced by the N-methyl-D-aspartate receptor 
      (NMDAR) overactivation is critical for cell death in SCI. It has been reported 
      that the magnesium ion (Mg(2+) ) can competitively block the NMDAR and reduce the 
      calcium influx, and that sonic hedgehog (Shh) and retinoic acid (RA) are the 
      critical regulators of neuronal differentiation of endogenous neural stem cells 
      (NSCs). Here, magnesium oxide (MgO)/poly (l-lactide-co-epsilon-caprolactone) (PLCL) 
      scaffold loaded with purmorphamine (PUR, a Shh signaling agonist) and RA is 
      developed and its feasibility in SCI repair is tested. The results showed that 
      the Mg(2+) released from MgO attenuated cell apoptosis by blocking the calcium 
      influx, and the PUR/RA promoted the recruitment and neuronal differentiation of 
      endogenous NSCs, thereby reducing the glial scar formation at the SCI lesion 
      site. Furthermore, implantation of PUR/RA-loaded MgO/PLCL scaffold facilitates 
      the partial recovery of a locomotor function of SCI mouse in vivo. Together, 
      findings from this study imply that PUR/RA-loaded MgO/PLCL scaffold may be a 
      promising biomaterial for the clinical treatment of SCI.
CI  - (c) 2022 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.
FAU - Xie, Jile
AU  - Xie J
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      899# Pinghai Road, Suzhou, Jiangsu, 215006, China.
FAU - Li, Jiaying
AU  - Li J
AD  - Orthopaedic Institute, Medical College, Soochow University, 1# Shizi Road, 
      Suzhou, Jiangsu, 215006, China.
FAU - Ma, Jinjin
AU  - Ma J
AD  - Orthopaedic Institute, Medical College, Soochow University, 1# Shizi Road, 
      Suzhou, Jiangsu, 215006, China.
FAU - Li, Meimei
AU  - Li M
AD  - Orthopaedic Institute, Medical College, Soochow University, 1# Shizi Road, 
      Suzhou, Jiangsu, 215006, China.
FAU - Wang, Xingran
AU  - Wang X
AD  - Orthopaedic Institute, Medical College, Soochow University, 1# Shizi Road, 
      Suzhou, Jiangsu, 215006, China.
FAU - Fu, Xinya
AU  - Fu X
AD  - Orthopaedic Institute, Medical College, Soochow University, 1# Shizi Road, 
      Suzhou, Jiangsu, 215006, China.
FAU - Ma, Yanxia
AU  - Ma Y
AD  - Orthopaedic Institute, Medical College, Soochow University, 1# Shizi Road, 
      Suzhou, Jiangsu, 215006, China.
FAU - Yang, Huilin
AU  - Yang H
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      899# Pinghai Road, Suzhou, Jiangsu, 215006, China.
AD  - Orthopaedic Institute, Medical College, Soochow University, 1# Shizi Road, 
      Suzhou, Jiangsu, 215006, China.
FAU - Li, Bin
AU  - Li B
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      899# Pinghai Road, Suzhou, Jiangsu, 215006, China.
AD  - Orthopaedic Institute, Medical College, Soochow University, 1# Shizi Road, 
      Suzhou, Jiangsu, 215006, China.
FAU - Saijilafu
AU  - Saijilafu
AUID- ORCID: 0000-0003-2290-9808
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      899# Pinghai Road, Suzhou, Jiangsu, 215006, China.
AD  - Orthopaedic Institute, Medical College, Soochow University, 1# Shizi Road, 
      Suzhou, Jiangsu, 215006, China.
LA  - eng
PT  - Journal Article
DEP - 20220603
PL  - Germany
TA  - Adv Healthc Mater
JT  - Advanced healthcare materials
JID - 101581613
RN  - 0 (Caproates)
RN  - 0 (Dioxanes)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Lactones)
RN  - 3A3U0GI71G (Magnesium Oxide)
RN  - 56RE988L1R (caprolactone)
RN  - 95-96-5 (dilactide)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Axons/metabolism
MH  - Calcium/metabolism
MH  - Caproates
MH  - Cell Differentiation
MH  - Dioxanes
MH  - Hedgehog Proteins/metabolism/pharmacology
MH  - Lactones
MH  - Magnesium Oxide/metabolism/pharmacology
MH  - Mice
MH  - *Neural Stem Cells
MH  - Spinal Cord/pathology
MH  - *Spinal Cord Injuries/drug therapy/pathology
MH  - *Spinal Cord Regeneration
MH  - Tissue Scaffolds
OTO - NOTNLM
OT  - magnesium
OT  - neural morphogens
OT  - neuroprotection
OT  - spinal cord injury
EDAT- 2022/05/20 06:00
MHDA- 2022/08/06 06:00
CRDT- 2022/05/19 05:52
PHST- 2022/04/22 00:00 [revised]
PHST- 2022/02/18 00:00 [received]
PHST- 2022/05/20 06:00 [pubmed]
PHST- 2022/08/06 06:00 [medline]
PHST- 2022/05/19 05:52 [entrez]
AID - 10.1002/adhm.202200386 [doi]
PST - ppublish
SO  - Adv Healthc Mater. 2022 Aug;11(15):e2200386. doi: 10.1002/adhm.202200386. Epub 
      2022 Jun 3.