PMID- 35588524
OWN - NLM
STAT- MEDLINE
DCOM- 20220523
LR  - 20220716
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 55
DP  - 2022
TI  - Lactate modulates cardiac gene expression in mice during acute physical exercise.
PG  - e11820
LID - S0100-879X2022000100630 [pii]
LID - 10.1590/1414-431X2022e11820 [doi]
LID - e11820
AB  - The aim of the present study was to verify the role of lactate as a signaling 
      molecule in cardiac tissue under physiological conditions. C57BL6/J male mice 
      were submitted to acute running bouts on a treadmill at different exercise 
      intensities (30, 60, and 90% of maximal speed - Smax) under the effect of two 
      doses (0.5 and 5 mM) of alpha-cyano-4-hydroxycynnamate (CINN), a blocker of lactate 
      transporters. Cardiac lactate levels, activity of the enzymes of glycolytic 
      [hexokinase (HK) and lactate dehydrogenase (LDH)] and oxidative metabolism 
      [citrate synthase (CS)], and expression of genes also related to metabolism [LDH, 
      nuclear factor erythroid 2-related factor 2 (NRF-2), cytochrome oxidase IV 
      (COX-IV), and peroxisome proliferator-activated receptor gamma coactivator 
      1-alpha (PGC-1alpha)] were evaluated. Elevated cardiac lactate levels were observed 
      after high intensity running at 90% of Smax, which were parallel to increased 
      activity of the HK and CS enzymes and mRNA levels of PGC-1alpha and COX-IV. No 
      changes were observed in cardiac lactate levels in mice running at lower exercise 
      intensities. Interestingly, prior intraperitoneal administration (15 min) of CINN 
      (0.5 mM) significantly reduced cardiac lactate concentration, activities of HK 
      and CS, and mRNA levels of PGC-1alpha and COX-IV in mice that ran at 90% of Smax. In 
      addition, cardiac lactate levels were significantly correlated to both PGC-1alpha and 
      COX-IV cardiac gene expression. The present study provides evidence that cardiac 
      lactate levels are associated to gene transcription during an acute bout of high 
      intensity running exercise.
FAU - Cunha, T F
AU  - Cunha TF
AUID- ORCID: 0000-0001-7542-093X
AD  - Escola de Educacao Fisica e Esporte, Universidade de Sao Paulo, Sao Paulo, SP, 
      Brasil.
AD  - Universidade Paulista, Sao Paulo, SP, Brasil.
FAU - Vieira, J S
AU  - Vieira JS
AUID- ORCID: 0000-0003-3508-6839
AD  - Escola de Educacao Fisica e Esporte, Universidade de Sao Paulo, Sao Paulo, SP, 
      Brasil.
FAU - Santos, J B
AU  - Santos JB
AUID- ORCID: 0000-0002-9119-6728
AD  - Escola de Educacao Fisica e Esporte, Universidade de Sao Paulo, Sao Paulo, SP, 
      Brasil.
FAU - Coelho, M A
AU  - Coelho MA
AUID- ORCID: 0000-0001-8581-8474
AD  - Escola de Educacao Fisica e Esporte, Universidade de Sao Paulo, Sao Paulo, SP, 
      Brasil.
FAU - Brum, P C
AU  - Brum PC
AUID- ORCID: 0000-0002-4750-6506
AD  - Escola de Educacao Fisica e Esporte, Universidade de Sao Paulo, Sao Paulo, SP, 
      Brasil.
FAU - Gabriel-Costa, D
AU  - Gabriel-Costa D
AUID- ORCID: 0000-0003-2961-9344
AD  - Escola de Educacao Fisica e Esporte, Universidade de Sao Paulo, Sao Paulo, SP, 
      Brasil.
AD  - Universidade da Forca Aerea, Forca Aerea Brasileira, Rio de Janeiro, RJ, Brasil.
LA  - eng
PT  - Journal Article
DEP - 20220427
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 33X04XA5AT (Lactic Acid)
RN  - EC 2.3.3.1 (Citrate (si)-Synthase)
SB  - IM
MH  - Animals
MH  - Citrate (si)-Synthase/genetics/metabolism/pharmacology
MH  - Gene Expression
MH  - Lactic Acid/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscle, Skeletal/metabolism
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 
      1-alpha/genetics/metabolism
MH  - *Physical Conditioning, Animal/physiology
MH  - RNA, Messenger/metabolism
MH  - *Transcription Factors/genetics/metabolism
PMC - PMC9054034
EDAT- 2022/05/20 06:00
MHDA- 2022/05/24 06:00
PMCR- 2022/04/27
CRDT- 2022/05/19 17:53
PHST- 2021/08/26 00:00 [received]
PHST- 2022/02/21 00:00 [accepted]
PHST- 2022/05/19 17:53 [entrez]
PHST- 2022/05/20 06:00 [pubmed]
PHST- 2022/05/24 06:00 [medline]
PHST- 2022/04/27 00:00 [pmc-release]
AID - S0100-879X2022000100630 [pii]
AID - 10.1590/1414-431X2022e11820 [doi]
PST - epublish
SO  - Braz J Med Biol Res. 2022 Apr 27;55:e11820. doi: 10.1590/1414-431X2022e11820. 
      eCollection 2022.