PMID- 35589822
OWN - NLM
STAT- MEDLINE
DCOM- 20220523
LR  - 20220716
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 May 19
TI  - Fluorescence in situ hybridization detection of chromosome 7 and/or 17 polysomy 
      as a prognostic marker for cholangiocarcinoma.
PG  - 8441
LID - 10.1038/s41598-022-11945-8 [doi]
LID - 8441
AB  - Cholangiocarcinoma (CCA) is highly endemic in the Northeast Thailand. Recently, 
      chromosome aberrations provided new insights into pathogenesis of CCA. Therefore, 
      chromosome aberration might be used as a prognostic factor and therapeutic 
      planning of this cancer. This aim of this study is to examine the correlation 
      between an increase of chromosome 7 (C7) and/or 17 (C17) copy number variants 
      (CNVs) with clinicopathological data and the overall survival time (OS) of CCA 
      patients using fluorescence in situ hybridization (FISH) assays. C7 and C17 CNVs 
      were examined using FISH form 157 formalin-fixed paraffin-embedded (FFPE) tissues 
      of CCA patients from Khon Kaen, Thailand between 2011 and 2015. OS was visualized 
      using Kaplan-Meier plot. Univariate and multivariate analyses were used to 
      determine the ability of the clinicopathological parameters to predict OS. 
      C17 > trisomy (odd ratio, 6.944, P < 0.001), C7/17 trisomy (odd ratio; 4.488, 
      P = 0.019), and C7/17 > trisomy (odd ratio; 6.723, P < 0.001) were independently 
      predictive factors for lymph node metastasis. Interestingly, an increase of C7, 
      C17, and C7/17 CNVs in both trisomy and > trisomy was independently correlated 
      with short median OS. An increased of C7 and/or 17 have a potential as a poor 
      prognostic marker in CCA patients.
CI  - (c) 2022. The Author(s).
FAU - Deenonpoe, Raksawan
AU  - Deenonpoe R
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, 123 
      Mittraparp Road, Muang District, Khon Kaen, 40002, Thailand. raksde@kku.ac.th.
AD  - Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, 
      Thailand. raksde@kku.ac.th.
FAU - Sa-Ngiamwibool, Prakasit
AU  - Sa-Ngiamwibool P
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, 123 
      Mittraparp Road, Muang District, Khon Kaen, 40002, Thailand.
AD  - Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, 
      Thailand.
FAU - Watcharadetwittaya, Sasithorn
AU  - Watcharadetwittaya S
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, 123 
      Mittraparp Road, Muang District, Khon Kaen, 40002, Thailand.
AD  - Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, 
      Thailand.
FAU - Thanee, Malinee
AU  - Thanee M
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, 123 
      Mittraparp Road, Muang District, Khon Kaen, 40002, Thailand.
AD  - Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, 
      Thailand.
FAU - Intuyod, Kitti
AU  - Intuyod K
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, 123 
      Mittraparp Road, Muang District, Khon Kaen, 40002, Thailand.
FAU - Kongpan, Thachanan
AU  - Kongpan T
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, 123 
      Mittraparp Road, Muang District, Khon Kaen, 40002, Thailand.
FAU - Padthaisong, Sureerat
AU  - Padthaisong S
AD  - Faculty of Allied Health Sciences, Burapha University, Chonburi, 20131, Thailand.
FAU - Nutalai, Rungtiwa
AU  - Nutalai R
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, 123 
      Mittraparp Road, Muang District, Khon Kaen, 40002, Thailand.
AD  - Nuffield Department of Medicine, Wellcome Trust Centre for Human Genetics, 
      University of Oxford, Oxford, UK.
FAU - Chamgramol, Yaovalux
AU  - Chamgramol Y
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, 123 
      Mittraparp Road, Muang District, Khon Kaen, 40002, Thailand.
FAU - Pairojkul, Chawalit
AU  - Pairojkul C
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, 123 
      Mittraparp Road, Muang District, Khon Kaen, 40002, Thailand.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220519
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - *Bile Duct Neoplasms/pathology
MH  - Bile Ducts, Intrahepatic/pathology
MH  - *Cholangiocarcinoma
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 7
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mosaicism
MH  - Prognosis
MH  - Thailand
MH  - Trisomy/pathology
PMC - PMC9119972
COIS- The authors declare no competing interests.
EDAT- 2022/05/20 06:00
MHDA- 2022/05/24 06:00
PMCR- 2022/05/19
CRDT- 2022/05/19 23:25
PHST- 2022/03/05 00:00 [received]
PHST- 2022/05/03 00:00 [accepted]
PHST- 2022/05/19 23:25 [entrez]
PHST- 2022/05/20 06:00 [pubmed]
PHST- 2022/05/24 06:00 [medline]
PHST- 2022/05/19 00:00 [pmc-release]
AID - 10.1038/s41598-022-11945-8 [pii]
AID - 11945 [pii]
AID - 10.1038/s41598-022-11945-8 [doi]
PST - epublish
SO  - Sci Rep. 2022 May 19;12(1):8441. doi: 10.1038/s41598-022-11945-8.