PMID- 35589912
OWN - NLM
STAT- MEDLINE
DCOM- 20220708
LR  - 20220716
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Print)
IS  - 0920-8569 (Linking)
VI  - 58
IP  - 4
DP  - 2022 Aug
TI  - Phylogenetic analysis of porcine reproductive and respiratory syndrome virus in 
      Vietnam, 2021.
PG  - 361-366
LID - 10.1007/s11262-022-01912-w [doi]
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV) causes more 
      economic losses in the swine industry than any other virus. This study aimed to 
      investigate the genetic diversity of PRRSV to assist in evaluating the 
      effectiveness of PRRS vaccines. Twenty-eight samples from clinical cases were 
      collected from 19 farms in seven provinces of Vietnam in 2021. Full-length PRRSV 
      ORF5 genes from the 19 samples were amplified, sequenced, and compared to the 
      corresponding sequences of referenced PRRSV strains from Genbank. The genetic 
      analysis showed that 12 isolates were the highly pathogenic PRRSV subtype 
      (HP-PRRSV) lineage 8, sublineage 8.7; six isolates were the classical North 
      American PRRSV subtype (US-PRRSV), NADC-like group, lineage 1, sublineage 1.4, 
      which were reported in Vietnam for the first time; and the final isolate was a 
      vaccine-like strain. The field isolates of HP-PRRSV had relatively higher genetic 
      diversity with US-PRRSV vaccine strains (84.0-94.5%) than HP-PRRSV vaccine 
      strains (95.3-98.6%). Meanwhile, the six NADC-like isolates had low nucleotide 
      similarity with US-PRRSV and HP-PRRSV vaccine strains (83.4-85.4% and 83.2-84.0%, 
      respectively). Many amino acid substitutions were found in antigenic regions of 
      GP5 involved in response to early antibody production, neutralizing antibodies, 
      and viral immune evasion between these field strains and PRRSV vaccine strains. 
      These findings provide insights into the molecular characteristics, genetic 
      diversity, antigenicity, and evolution of PRRSV strains in Vietnam and postulate 
      a compelling explanation for the limitations of current vaccination efforts.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Nguyen, Ngoc Hai
AU  - Nguyen NH
AD  - Department of Infectious Disease and Veterinary Public Health, Faculty of Animal 
      Husbandry and Veterinary Medicine, Nong Lam University, Linh Trung Ward, Thu Duc 
      City, Quarter 6, Ho Chi Minh City, Vietnam. nguyenngochai@hcmuaf.edu.vn.
AD  - P&Y Livestock Service Company Limited, Ho Chi Minh City, Vietnam. 
      nguyenngochai@hcmuaf.edu.vn.
FAU - Tran, Hoang Anh Thu
AU  - Tran HAT
AD  - P&Y Livestock Service Company Limited, Ho Chi Minh City, Vietnam.
FAU - Nguyen, Trung Quan
AU  - Nguyen TQ
AD  - P&Y Livestock Service Company Limited, Ho Chi Minh City, Vietnam.
FAU - Nguyen, Phuong Binh Thi
AU  - Nguyen PBT
AD  - P&Y Livestock Service Company Limited, Ho Chi Minh City, Vietnam.
FAU - Le, Thu Ha Thi
AU  - Le THT
AD  - MSD Animal Health Vietnam Company, Ho Chi Minh City, Vietnam.
FAU - Lai, Danh Cong
AU  - Lai DC
AD  - Department of Infectious Disease and Veterinary Public Health, Faculty of Animal 
      Husbandry and Veterinary Medicine, Nong Lam University, Linh Trung Ward, Thu Duc 
      City, Quarter 6, Ho Chi Minh City, Vietnam.
FAU - Nguyen, Minh Nam
AU  - Nguyen MN
AUID- ORCID: 0000-0001-5596-6222
AD  - Department of Biomedical Engineering, School of Medicine, Vietnam National 
      University Ho Chi Minh City (VNU-HCM), Ho Chi Minh City, 700000, Vietnam. 
      nmnam@medvnu.edu.vn.
AD  - Research Center for Genetics and Reproductive Health (CGRH), School of Medicine, 
      National University-HCMC, Ho Chi Minh City, Vietnam. nmnam@medvnu.edu.vn.
AD  - Vietnam National University Ho Chi Minh City (VNU-HCM), Ho Chi Minh City, 700000, 
      Vietnam. nmnam@medvnu.edu.vn.
LA  - eng
PT  - Journal Article
DEP - 20220519
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Genetic Variation
MH  - Phylogeny
MH  - *Porcine Reproductive and Respiratory Syndrome/epidemiology
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - Swine
MH  - Vietnam/epidemiology
PMC - PMC9119219
OTO - NOTNLM
OT  - Genetic diversity
OT  - ORF5
OT  - PRRSV
OT  - Vaccine
OT  - phylogenetic analysis
COIS- All authors have read the journal's policy on disclosures of potential conflicts 
      of interest, and we declare no potential conflicts of interest with respect to 
      the research, authorship, and/or publication of this article.
EDAT- 2022/05/20 06:00
MHDA- 2022/07/09 06:00
PMCR- 2022/05/19
CRDT- 2022/05/19 23:29
PHST- 2022/02/14 00:00 [received]
PHST- 2022/05/05 00:00 [accepted]
PHST- 2022/05/20 06:00 [pubmed]
PHST- 2022/07/09 06:00 [medline]
PHST- 2022/05/19 23:29 [entrez]
PHST- 2022/05/19 00:00 [pmc-release]
AID - 10.1007/s11262-022-01912-w [pii]
AID - 1912 [pii]
AID - 10.1007/s11262-022-01912-w [doi]
PST - ppublish
SO  - Virus Genes. 2022 Aug;58(4):361-366. doi: 10.1007/s11262-022-01912-w. Epub 2022 
      May 19.