PMID- 35590057
OWN - NLM
STAT- MEDLINE
DCOM- 20220523
LR  - 20220716
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 May 19
TI  - Initial TK-deficient HSV-1 infection in the lip alters contralateral lip 
      challenge immune dynamics.
PG  - 8489
LID - 10.1038/s41598-022-12597-4 [doi]
LID - 8489
AB  - Primary infection with herpes simplex type 1 (HSV-1) occurring around the mouth 
      and nose switches rapidly to lifelong latent infection in sensitive trigeminal 
      ganglia (TG) neurons. Sporadic reactivation of these latent reservoirs later in 
      life is the cause of acute infections of the corneal epithelium, which can cause 
      potentially blinding herpes simplex keratitis (HSK). There is no effective 
      vaccine to protect against HSK, and antiviral drugs provide only partial 
      protection against recurrences. We previously engendered an acute disease-free, 
      non-reactivating latent state in mice when challenged with virulent HSV-1 in 
      orofacial mucosa, by priming with non-neurovirulent HSV-1 (TK(del)) before the 
      challenge. Herein, we define the local immune infiltration and inflammatory 
      chemokine production changes after virulent HSV-1 challenge, which were elicited 
      by TK(del) prime. Heightened immunosurveillance before virulent challenge, and 
      early enhanced lymphocyte-enriched infiltration of the challenged lip were 
      induced, which corresponded to attenuation of inflammation in the TG and enhanced 
      viral control. Furthermore, classical latent-phase T cell persistence around 
      latent HSV-1 reservoirs were severely reduced. These findings identify the immune 
      processes that are likely to be responsible for establishing non-reactivating 
      latent HSV-1 reservoirs. Stopping reactivation is essential for development of 
      efficient vaccine strategies against HSV-1.
CI  - (c) 2022. The Author(s).
FAU - Rousseau, Antoine
AU  - Rousseau A
AD  - Center for Immunology of Viral, Auto-immune, Hematological and Bacterial Diseases 
      (IMVA-HB), Commissariat a l'energie Atomique et Aux Energies Renouvelables, 
      Universite Paris-Saclay, INSERM U1184, Fontenay-aux-Roses, France.
AD  - Service d'Ophtalmologie, Hopital Bicetre, APHP, Universite Paris-Saclay, Le 
      Kremlin-Bicetre, France.
FAU - Haigh, Oscar
AU  - Haigh O
AD  - Center for Immunology of Viral, Auto-immune, Hematological and Bacterial Diseases 
      (IMVA-HB), Commissariat a l'energie Atomique et Aux Energies Renouvelables, 
      Universite Paris-Saclay, INSERM U1184, Fontenay-aux-Roses, France. 
      Oscar.Haigh@cea.fr.
FAU - Legrand, Roger
AU  - Legrand R
AD  - Center for Immunology of Viral, Auto-immune, Hematological and Bacterial Diseases 
      (IMVA-HB), Commissariat a l'energie Atomique et Aux Energies Renouvelables, 
      Universite Paris-Saclay, INSERM U1184, Fontenay-aux-Roses, France.
FAU - Palgen, Jean-Louis
AU  - Palgen JL
AD  - Center for Immunology of Viral, Auto-immune, Hematological and Bacterial Diseases 
      (IMVA-HB), Commissariat a l'energie Atomique et Aux Energies Renouvelables, 
      Universite Paris-Saclay, INSERM U1184, Fontenay-aux-Roses, France.
FAU - Lemaitre, Julien
AU  - Lemaitre J
AD  - Center for Immunology of Viral, Auto-immune, Hematological and Bacterial Diseases 
      (IMVA-HB), Commissariat a l'energie Atomique et Aux Energies Renouvelables, 
      Universite Paris-Saclay, INSERM U1184, Fontenay-aux-Roses, France.
FAU - Deback, Claire
AU  - Deback C
AD  - Service de Virologie, Hopital Paul Brousse, Universite Paris-Saclay, APHP, 
      Villejuif, France.
FAU - Oziol, Noemie
AU  - Oziol N
AD  - Center for Immunology of Viral, Auto-immune, Hematological and Bacterial Diseases 
      (IMVA-HB), Commissariat a l'energie Atomique et Aux Energies Renouvelables, 
      Universite Paris-Saclay, INSERM U1184, Fontenay-aux-Roses, France.
FAU - Lomonte, Patrick
AU  - Lomonte P
AD  - Institut NeuroMyoGene-Pathophysiology and Genetics of Neuron and Muscle 
      (INMG-PGNM), CNRS UMR 5261, INSERM U 1513, Universite Claude Bernard Lyon 1, Team 
      Chromatin Dynamics, Nuclear Domains, Virus, Lyon, France.
FAU - Labetoulle, Marc
AU  - Labetoulle M
AD  - Center for Immunology of Viral, Auto-immune, Hematological and Bacterial Diseases 
      (IMVA-HB), Commissariat a l'energie Atomique et Aux Energies Renouvelables, 
      Universite Paris-Saclay, INSERM U1184, Fontenay-aux-Roses, France. 
      Marc.Labetoulle@aphp.fr.
AD  - Service d'Ophtalmologie, Hopital Bicetre, APHP, Universite Paris-Saclay, Le 
      Kremlin-Bicetre, France. Marc.Labetoulle@aphp.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220519
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Animals
MH  - *Herpes Simplex
MH  - *Herpesvirus 1, Human/physiology
MH  - *Keratitis, Herpetic
MH  - Lip
MH  - Mice
MH  - Trigeminal Ganglion
PMC - PMC9119387
COIS- The authors declare no competing interests.
EDAT- 2022/05/20 06:00
MHDA- 2022/05/24 06:00
PMCR- 2022/05/19
CRDT- 2022/05/19 23:37
PHST- 2022/02/03 00:00 [received]
PHST- 2022/05/12 00:00 [accepted]
PHST- 2022/05/19 23:37 [entrez]
PHST- 2022/05/20 06:00 [pubmed]
PHST- 2022/05/24 06:00 [medline]
PHST- 2022/05/19 00:00 [pmc-release]
AID - 10.1038/s41598-022-12597-4 [pii]
AID - 12597 [pii]
AID - 10.1038/s41598-022-12597-4 [doi]
PST - epublish
SO  - Sci Rep. 2022 May 19;12(1):8489. doi: 10.1038/s41598-022-12597-4.