PMID- 35591655
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 1996-1944 (Print)
IS  - 1996-1944 (Electronic)
IS  - 1996-1944 (Linking)
VI  - 15
IP  - 9
DP  - 2022 May 5
TI  - Photocurable Hydrogel Substrate-Better Potential Substitute on 
      Bone-Marrow-Derived Dendritic Cells Culturing.
LID - 10.3390/ma15093322 [doi]
LID - 3322
AB  - Dendritic cells (DCs) are recognized as the most effective antigen-presenting 
      cells at present. DCs have corresponding therapeutic effects in tumor immunity, 
      transplantation immunity, infection inflammation and cardiovascular diseases, and 
      the activation of T cells is dependent on DCs. However, normal 
      bone-marrow-derived Dendritic cells (BMDCs) cultured on conventional culture 
      plates are easy to be activated during culturing, and it is difficult to imitate 
      the internal immune function. Here, we reported a novel BMDCs culturing with 
      hydrogel substrate (CCHS), where we synthesized low substituted Gelatin 
      Methacrylate-30 (GelMA-30) hydrogels and used them as a substitute for 
      conventional culture plates in the culture and induction of BMDCs in vitro. The 
      results showed that 5% GelMA-30 substrate was the best culture condition for 
      BMDCs culturing. The low level of costimulatory molecules and the level of 
      development-related transcription factors of BMDCs by CCHS were closer to that of 
      spleen DCs and were capable of better promoting T cell activation and exerting an 
      immune effect. CCHS was helpful to study the transformation of DCs from initial 
      state to activated state, which contributes to the development of DC-T cell 
      immunotherapy.
FAU - Deng, Jiewen
AU  - Deng J
AD  - Department of Cardiology, The Second Affiliated Hospital, Zhejiang University 
      School of Medicine, 88 Jiefang Road, Hangzhou 310009, China.
FAU - Xie, Yao
AU  - Xie Y
AD  - Department of Cardiology, The Second Affiliated Hospital, Zhejiang University 
      School of Medicine, 88 Jiefang Road, Hangzhou 310009, China.
FAU - Shen, Jian
AU  - Shen J
AD  - Department of Cardiology, The Second Affiliated Hospital, Zhejiang University 
      School of Medicine, 88 Jiefang Road, Hangzhou 310009, China.
FAU - Gao, Qing
AU  - Gao Q
AD  - Engineering for Life Group (EFL), Suzhou 215000, China.
FAU - He, Jing
AU  - He J
AD  - State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical 
      Engineering, Zhejiang University, Hangzhou 310027, China.
AD  - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School 
      of Mechanical Engineering, Zhejiang University, Hangzhou 310027, China.
FAU - Ma, Hong
AU  - Ma H
AUID- ORCID: 0000-0003-3419-4627
AD  - Department of Cardiology, The Second Affiliated Hospital, Zhejiang University 
      School of Medicine, 88 Jiefang Road, Hangzhou 310009, China.
FAU - Ji, Yongli
AU  - Ji Y
AD  - Department of Cardiology, The Second Affiliated Hospital, Zhejiang University 
      School of Medicine, 88 Jiefang Road, Hangzhou 310009, China.
FAU - He, Yong
AU  - He Y
AUID- ORCID: 0000-0002-9099-0831
AD  - State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical 
      Engineering, Zhejiang University, Hangzhou 310027, China.
AD  - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School 
      of Mechanical Engineering, Zhejiang University, Hangzhou 310027, China.
FAU - Xiang, Meixiang
AU  - Xiang M
AD  - Department of Cardiology, The Second Affiliated Hospital, Zhejiang University 
      School of Medicine, 88 Jiefang Road, Hangzhou 310009, China.
LA  - eng
GR  - U1909218, T212100/3D printing/
GR  - 81870203/HMGXB4/
GR  - 81670259/Piezo1/
PT  - Journal Article
DEP - 20220505
PL  - Switzerland
TA  - Materials (Basel)
JT  - Materials (Basel, Switzerland)
JID - 101555929
PMC - PMC9104740
OTO - NOTNLM
OT  - DCs function
OT  - GelMA-30
OT  - a novel BMDCs culturing with hydrogel substrate (CCHS)
COIS- We declare that we have no financial and personal relationship with other people 
      or organizations that can inappropriately influence our work, there is no 
      professional or other personal interest of any nature or kind in any product, 
      service and/or company that could be construed as influencing the position 
      presented in, or the review of, the manuscript entitled "Photocurable Hydrogel 
      Substrate-Better Potential Substitute on Bone-marrow-derived Dendritic Cells 
      Culturing".
EDAT- 2022/05/21 06:00
MHDA- 2022/05/21 06:01
PMCR- 2022/05/05
CRDT- 2022/05/20 01:14
PHST- 2022/02/13 00:00 [received]
PHST- 2022/04/06 00:00 [revised]
PHST- 2022/04/27 00:00 [accepted]
PHST- 2022/05/20 01:14 [entrez]
PHST- 2022/05/21 06:00 [pubmed]
PHST- 2022/05/21 06:01 [medline]
PHST- 2022/05/05 00:00 [pmc-release]
AID - ma15093322 [pii]
AID - materials-15-03322 [pii]
AID - 10.3390/ma15093322 [doi]
PST - epublish
SO  - Materials (Basel). 2022 May 5;15(9):3322. doi: 10.3390/ma15093322.