PMID- 35596080
OWN - NLM
STAT- MEDLINE
DCOM- 20220524
LR  - 20220930
IS  - 2055-0278 (Electronic)
IS  - 2055-0278 (Linking)
VI  - 8
IP  - 5
DP  - 2022 May
TI  - Modelling the pyrenoid-based CO(2)-concentrating mechanism provides insights into 
      its operating principles and a roadmap for its engineering into crops.
PG  - 583-595
LID - 10.1038/s41477-022-01153-7 [doi]
AB  - Many eukaryotic photosynthetic organisms enhance their carbon uptake by supplying 
      concentrated CO(2) to the CO(2)-fixing enzyme Rubisco in an organelle called the 
      pyrenoid. Ongoing efforts seek to engineer this pyrenoid-based 
      CO(2)-concentrating mechanism (PCCM) into crops to increase yields. Here we 
      develop a computational model for a PCCM on the basis of the postulated mechanism 
      in the green alga Chlamydomonas reinhardtii. Our model recapitulates all 
      Chlamydomonas PCCM-deficient mutant phenotypes and yields general biophysical 
      principles underlying the PCCM. We show that an effective and energetically 
      efficient PCCM requires a physical barrier to reduce pyrenoid CO(2) leakage, as 
      well as proper enzyme localization to reduce futile cycling between CO(2) and 
      HCO(3)(-). Importantly, our model demonstrates the feasibility of a purely 
      passive CO(2) uptake strategy at air-level CO(2), while active HCO(3)(-) uptake 
      proves advantageous at lower CO(2) levels. We propose a four-step engineering 
      path to increase the rate of CO(2) fixation in the plant chloroplast up to 
      threefold at a theoretical cost of only 1.3 ATP per CO(2) fixed, thereby offering 
      a framework to guide the engineering of a PCCM into land plants.
CI  - (c) 2022. The Author(s).
FAU - Fei, Chenyi
AU  - Fei C
AUID- ORCID: 0000-0002-8287-4347
AD  - Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
AD  - Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, 
      NJ, USA.
FAU - Wilson, Alexandra T
AU  - Wilson AT
AUID- ORCID: 0000-0002-1676-000X
AD  - Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
AD  - Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
FAU - Mangan, Niall M
AU  - Mangan NM
AUID- ORCID: 0000-0002-3491-8341
AD  - Department of Engineering Sciences and Applied Mathematics, Northwestern 
      University, Evanston, IL, USA. niall.mangan@northwestern.edu.
FAU - Wingreen, Ned S
AU  - Wingreen NS
AUID- ORCID: 0000-0001-7384-2821
AD  - Department of Molecular Biology, Princeton University, Princeton, NJ, USA. 
      wingreen@princeton.edu.
AD  - Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, 
      NJ, USA. wingreen@princeton.edu.
FAU - Jonikas, Martin C
AU  - Jonikas MC
AUID- ORCID: 0000-0002-9519-6055
AD  - Department of Molecular Biology, Princeton University, Princeton, NJ, USA. 
      mjonikas@princeton.edu.
AD  - Howard Hughes Medical Institute, Princeton University, Princeton, NJ, USA. 
      mjonikas@princeton.edu.
LA  - eng
GR  - R01 GM140032/GM/NIGMS NIH HHS/United States
GR  - 55108535/HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
DEP - 20220519
PL  - England
TA  - Nat Plants
JT  - Nature plants
JID - 101651677
RN  - 142M471B3J (Carbon Dioxide)
RN  - EC 4.1.1.39 (Ribulose-Bisphosphate Carboxylase)
SB  - IM
MH  - *Carbon Dioxide/metabolism
MH  - *Chlamydomonas reinhardtii/genetics/metabolism
MH  - Chloroplasts/metabolism
MH  - Crops, Agricultural/genetics/metabolism
MH  - Photosynthesis/genetics
MH  - Plastids/metabolism
MH  - Ribulose-Bisphosphate Carboxylase/genetics/metabolism
PMC - PMC9122830
COIS- The authors declare no competing interests.
EDAT- 2022/05/21 06:00
MHDA- 2022/05/25 06:00
PMCR- 2022/05/19
CRDT- 2022/05/20 23:33
PHST- 2021/08/04 00:00 [received]
PHST- 2022/04/11 00:00 [accepted]
PHST- 2022/05/20 23:33 [entrez]
PHST- 2022/05/21 06:00 [pubmed]
PHST- 2022/05/25 06:00 [medline]
PHST- 2022/05/19 00:00 [pmc-release]
AID - 10.1038/s41477-022-01153-7 [pii]
AID - 1153 [pii]
AID - 10.1038/s41477-022-01153-7 [doi]
PST - ppublish
SO  - Nat Plants. 2022 May;8(5):583-595. doi: 10.1038/s41477-022-01153-7. Epub 2022 May 
      19.