PMID- 35597302
OWN - NLM
STAT- MEDLINE
DCOM- 20220615
LR  - 20220809
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 134
DP  - 2022 Sep
TI  - Expression analysis of grass carp Foxp3 and its biologic effects on CXCL-8 
      transcription in non-lymphoid cells.
PG  - 104447
LID - S0145-305X(22)00109-4 [pii]
LID - 10.1016/j.dci.2022.104447 [doi]
AB  - Teleost Forkhead box protein P3 (Foxp3) expression was discovered not only in 
      regulatory T cells (Tregs) but also in other cells. Compared to the extensive 
      study on its roles in lymphoid cells, the expression pattern and biological roles 
      of Foxp3 in non-lymphoid cells have not been elucidated in both mammals and fish 
      species. In the present study, grass carp Foxp3 (gcFoxp3) mRNA expression was 
      detected in different cell types, showing that it has a moderate expression level 
      in peripheral blood leukocytes (PBLs), head kidney leukocytes (HKLs) and grass 
      carp fibroblast-like kidney cells (CIK cells). Interestingly, gcFoxp3 mRNA and 
      protein expression could be significantly stimulated by 
      polyinosinic-polycytidylic acid (poly I:C) in CIK cells, indicating its 
      participation in poly I:C-induced immune response in non-lymphoid cells. To 
      further investigate the function of gcFoxp3, its overexpression plasmid was 
      constructed and transfected into CIK cells. After 24 h of transfection, grass 
      carp C-X-C chemokine ligand (CXCL) 8 (gcCXCL-8) mRNA expression was elevated, 
      implying the modulatory role of gcFoxp3 in gcCXCL-8 mRNA expression. This notion 
      was further supported by the features of gcCXCL-8 promoter which contained a 
      putative Foxp3 binding site at -2196 to -2190 region. Poly I:C or overexpression 
      of gcFoxp3 obviously stimulated gcCXCL-8 promoter activity and deletion of 
      gcFoxp3 binding region on the promoter abolished this stimulation, revealing that 
      Foxp3 is pivotal for transcription of CXCL-8 induced by poly I:C. In conclusion, 
      our results collectively demonstrate expression pattern of teleost Foxp3, and 
      illuminate novel immune function of fish Foxp3 in regulating chemokine 
      transcription in non-lymphoid cells.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Wei, He
AU  - Wei H
AD  - School of Bioscience and Technology, Chengdu Medical College, Chengdu, People's 
      Republic of China; Department of Gastroenterology, The Second Affiliated Hospital 
      of Chengdu Medical College, Chengdu, People's Republic of China. Electronic 
      address: weihe@cmc.edu.cn.
FAU - Qiu, Xingyang
AU  - Qiu X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Lv, Mengyuan
AU  - Lv M
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Liu, Xuelian
AU  - Liu X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220518
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Biological Products)
RN  - 0 (Fish Proteins)
RN  - 0 (Ligands)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Animals
MH  - *Biological Products
MH  - *Carps/genetics/metabolism
MH  - Fish Proteins/metabolism
MH  - Ligands
MH  - Mammals/genetics
MH  - Poly I-C/pharmacology
MH  - RNA, Messenger
MH  - Transcription Factors
OTO - NOTNLM
OT  - CXCL-8
OT  - Foxp3
OT  - Grass carp
OT  - Non-lymphocyte
OT  - Transcriptional regulation
EDAT- 2022/05/22 06:00
MHDA- 2022/06/16 06:00
CRDT- 2022/05/21 19:23
PHST- 2021/12/12 00:00 [received]
PHST- 2022/05/13 00:00 [revised]
PHST- 2022/05/13 00:00 [accepted]
PHST- 2022/05/22 06:00 [pubmed]
PHST- 2022/06/16 06:00 [medline]
PHST- 2022/05/21 19:23 [entrez]
AID - S0145-305X(22)00109-4 [pii]
AID - 10.1016/j.dci.2022.104447 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2022 Sep;134:104447. doi: 10.1016/j.dci.2022.104447. Epub 2022 
      May 18.