PMID- 35598284
OWN - NLM
STAT- MEDLINE
DCOM- 20220719
LR  - 20220909
IS  - 1550-7408 (Electronic)
IS  - 1066-5234 (Linking)
VI  - 69
IP  - 4
DP  - 2022 Jul
TI  - Investigation of the genome sizes and ploidy within the genus Monocercomonoides.
PG  - e12925
LID - 10.1111/jeu.12925 [doi]
AB  - Monocercomonoides is a genus of anaerobic flagellates found mainly in the gut of 
      insects and vertebrates. We explored the ploidy of six strains of 
      Monocercomonoides using fluorescence in situ hybridization (FISH) with probes 
      against the SufDSU gene known to be in a single copy in M. exilis. Our results 
      show that all investigated strains are haploid, with a single clear signal 
      displayed in most of the analyzed nuclei. Staining of the telomeric repeats 
      TTAGGG using FISH revealed that all investigated strains, except for strains of 
      M. merkovicensis, exhibit a similar number of telomeric signals to those of 
      M. exilis. DNA content of the nuclei in seven strains was assessed using flow 
      cytometry. With the knowledge of the ploidy, their haploid genome sizes were 
      estimated to vary from 60 to 161 Mbp. The genome size variation observed in 
      Monocercomonoides is much larger than the variation within other genera of 
      metamonads such as Trichomonas, Tritrichomonas, or Giardia, but similar to the 
      variations observed within genera of algae or plants.
CI  - (c) 2022 International Society of Protistologists.
FAU - Kornalikova, Martina
AU  - Kornalikova M
AD  - Department of Parasitology, Faculty of Science, BIOCEV, Charles University, 
      Vestec, Czech Republic.
FAU - Hampl, Vladimir
AU  - Hampl V
AUID- ORCID: 0000-0002-5430-7564
AD  - Department of Parasitology, Faculty of Science, BIOCEV, Charles University, 
      Vestec, Czech Republic.
FAU - Treitli, Sebastian Cristian
AU  - Treitli SC
AUID- ORCID: 0000-0001-8000-1780
AD  - Department of Parasitology, Faculty of Science, BIOCEV, Charles University, 
      Vestec, Czech Republic.
LA  - eng
GR  - CZ.02.1.01/0.0/0.0/16_019/0000759/Centre for research of pathogenicity and 
      virulence of parasites/
GR  - 771592/H2020 European Research Council/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220606
PL  - United States
TA  - J Eukaryot Microbiol
JT  - The Journal of eukaryotic microbiology
JID - 9306405
SB  - IM
MH  - Animals
MH  - Genome Size
MH  - In Situ Hybridization, Fluorescence
MH  - *Oxymonadida/genetics
MH  - Plants/genetics
MH  - Ploidies
OTO - NOTNLM
OT  - evolution
OT  - genome size variation
OT  - haploid
OT  - karyotype
OT  - oxymonads
EDAT- 2022/05/23 06:00
MHDA- 2022/07/20 06:00
CRDT- 2022/05/22 14:01
PHST- 2022/05/23 06:00 [pubmed]
PHST- 2022/07/20 06:00 [medline]
PHST- 2022/05/22 14:01 [entrez]
AID - 10.1111/jeu.12925 [doi]
PST - ppublish
SO  - J Eukaryot Microbiol. 2022 Jul;69(4):e12925. doi: 10.1111/jeu.12925. Epub 2022 
      Jun 6.