PMID- 35599260
OWN - NLM
STAT- MEDLINE
DCOM- 20220617
LR  - 20220617
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 106
IP  - 11
DP  - 2022 Jun
TI  - Preparation and identification of monoclonal antibodies against porcine CD103.
PG  - 4005-4015
LID - 10.1007/s00253-022-11950-x [doi]
AB  - Dendritic cells (DCs) play an important role in activating, regulating, and 
      maintaining the immune response. CD103+ DCs, one of the DC subpopulations, mainly 
      function in the mucosal immune response. They are responsible for capturing and 
      carrying antigens to the relevant lymph nodes to activate the downstream immune 
      responses. However, there is limited available information regarding the function 
      of CD103+ DCs in the porcine mucosal immune response. In this study, two 
      monoclonal antibodies (mAbs) against porcine CD103 were prepared, and their 
      applications were evaluated by enzyme-linked immunosorbent assay (ELISA), 
      indirect immunofluorescence assay (IFA), and flow cytometry. The produced mAbs 
      (7F3 and 9H3) were both IgG1 subtype with kappa chains in the light chain. The 7F3 
      recognizes a linear epitope (PDLRPRAQVYFSDLE) while 9H3 recognizes another linear 
      epitope (QILDEGQVLLGAVGA). The prepared mAbs could be used in vivo to detect the 
      cells expressing CD103 molecules, giving wide applications of both mAbs. In 
      conclusion, this study successfully prepared 2 mAbs against CD103 protein, and 
      they showed applicability in vivo experiments, which will provide the basis for 
      the study of porcine mucosal immunity. KEY POINTS: * Preparation of monoclonal 
      antibodies against porcine CD103 molecule * Analysis of the distribution of CD103 
      protein on different cells is possible * Exploration of the CD103(+) DCs function 
      in porcine mucosal immunity is possible.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Zhang, Tao
AU  - Zhang T
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Yanchanghui 
      Chengguan District Lanzhou, 1 Xujiaping, Gansu, 730046, China.
FAU - Yu, Haoyuan
AU  - Yu H
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Yanchanghui 
      Chengguan District Lanzhou, 1 Xujiaping, Gansu, 730046, China.
FAU - Aryal, Manita
AU  - Aryal M
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Yanchanghui 
      Chengguan District Lanzhou, 1 Xujiaping, Gansu, 730046, China.
FAU - Yang, Jing
AU  - Yang J
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Yanchanghui 
      Chengguan District Lanzhou, 1 Xujiaping, Gansu, 730046, China.
FAU - Li, Maolin
AU  - Li M
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Yanchanghui 
      Chengguan District Lanzhou, 1 Xujiaping, Gansu, 730046, China.
FAU - Li, Shuxian
AU  - Li S
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Yanchanghui 
      Chengguan District Lanzhou, 1 Xujiaping, Gansu, 730046, China.
FAU - Zhang, Na
AU  - Zhang N
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Yanchanghui 
      Chengguan District Lanzhou, 1 Xujiaping, Gansu, 730046, China.
FAU - Shi, Han
AU  - Shi H
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Yanchanghui 
      Chengguan District Lanzhou, 1 Xujiaping, Gansu, 730046, China.
FAU - Li, Baoyu
AU  - Li B
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Yanchanghui 
      Chengguan District Lanzhou, 1 Xujiaping, Gansu, 730046, China.
FAU - Liu, Guangliang
AU  - Liu G
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Yanchanghui 
      Chengguan District Lanzhou, 1 Xujiaping, Gansu, 730046, China.
FAU - Fu, Yuguang
AU  - Fu Y
AUID- ORCID: 0000-0003-3204-7156
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Yanchanghui 
      Chengguan District Lanzhou, 1 Xujiaping, Gansu, 730046, China. fuyuguang@caas.cn.
LA  - eng
GR  - 20JR10RA020/Key Science and Technology Foundation of Gansu Province/
PT  - Journal Article
DEP - 20220523
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
SB  - IM
MH  - Animals
MH  - *Antibodies, Monoclonal/metabolism
MH  - *Dendritic Cells
MH  - Epitopes/metabolism
MH  - Immunity, Mucosal
MH  - Lymph Nodes/metabolism
MH  - Swine
OTO - NOTNLM
OT  - CD103
OT  - Dendritic cells
OT  - mAbs
EDAT- 2022/05/23 06:00
MHDA- 2022/06/18 06:00
CRDT- 2022/05/22 23:13
PHST- 2022/01/19 00:00 [received]
PHST- 2022/05/02 00:00 [accepted]
PHST- 2022/04/27 00:00 [revised]
PHST- 2022/05/23 06:00 [pubmed]
PHST- 2022/06/18 06:00 [medline]
PHST- 2022/05/22 23:13 [entrez]
AID - 10.1007/s00253-022-11950-x [pii]
AID - 10.1007/s00253-022-11950-x [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2022 Jun;106(11):4005-4015. doi: 
      10.1007/s00253-022-11950-x. Epub 2022 May 23.