PMID- 35600094
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230916
IS  - 2057-1577 (Print)
IS  - 2057-1577 (Electronic)
IS  - 2057-1577 (Linking)
VI  - 8
IP  - 1
DP  - 2022
TI  - Genetic diversity and connectivity of the Ostreid herpesvirus 1 populations in 
      France: A first attempt to phylogeographic inference for a marine mollusc 
      disease.
PG  - veac039
LID - 10.1093/ve/veac039 [doi]
LID - veac039
AB  - The genetic diversity of viral populations is a key driver of the spatial and 
      temporal diffusion of viruses; yet, studying the diversity of whole genomes from 
      natural populations still remains a challenge. Phylodynamic approaches are 
      commonly used for RNA viruses harboring small genomes but have only rarely been 
      applied to DNA viruses with larger genomes. Here, we used the Pacific oyster 
      mortality syndrome (a disease that affects oyster farms around the world) as a 
      model to study the genetic diversity of its causative agent, the Ostreid 
      herpesvirus 1 (OsHV-1) in the three main French oyster-farming areas. Using 
      ultra-deep sequencing on individual moribund oysters and an innovative 
      combination of bioinformatics tools, we de novo assembled twenty-one OsHV-1 new 
      genomes. Combining quantification of major and minor genetic variations, 
      phylogenetic analysis, and ancestral state reconstruction of discrete traits 
      approaches, we assessed the connectivity of OsHV-1 viral populations between the 
      three oyster-farming areas. Our results suggest that the Marennes-Oleron Bay 
      represents the main source of OsHV-1 diversity, from where the virus has 
      dispersed to other farming areas, a scenario consistent with current practices of 
      oyster transfers in France. We demonstrate that phylodynamic approaches can be 
      applied to aquatic DNA viruses to determine how epidemiological, immunological, 
      and evolutionary processes act and potentially interact to shape their diversity 
      patterns.
CI  - (c) The Author(s) 2022. Published by Oxford University Press.
FAU - Delmotte, Jean
AU  - Delmotte J
FAU - Pelletier, Camille
AU  - Pelletier C
AD  - Ifremer, RBE-ASIM, Station La Tremblade, La Tremblade F-17390, France.
FAU - Morga, Benjamin
AU  - Morga B
AD  - Ifremer, RBE-ASIM, Station La Tremblade, La Tremblade F-17390, France.
FAU - Galinier, Richard
AU  - Galinier R
AD  - IHPE, CNRS, Ifremer, UPVD, University of Montpellier, Perpignan F-66000, France.
FAU - Petton, Bruno
AU  - Petton B
AD  - Ifremer, CNRS, IRD, Ifremer, LEMAR UMR 6539 Universite de Bretagne Occidentale, 
      Argenton-en-Landunvez F-29840, France.
FAU - Lamy, Jean-Baptiste
AU  - Lamy JB
AD  - Ifremer, RBE-ASIM, Station La Tremblade, La Tremblade F-17390, France.
FAU - Kaltz, Oliver
AU  - Kaltz O
AD  - ISEM, IRD, CNRS, University of Montpellier, Montpellier F-34095, France.
FAU - Avarre, Jean-Christophe
AU  - Avarre JC
AUID- ORCID: 0000-0001-6899-7052
AD  - ISEM, IRD, CNRS, University of Montpellier, Montpellier F-34095, France.
FAU - Jacquot, Maude
AU  - Jacquot M
AUID- ORCID: 0000-0001-5945-4689
AD  - Ifremer, RBE-ASIM, Station La Tremblade, La Tremblade F-17390, France.
FAU - Montagnani, Caroline
AU  - Montagnani C
AD  - IHPE, CNRS, Ifremer, UPVD, University of Montpellier, Montpellier F-34095, 
      France.
FAU - Escoubas, Jean-Michel
AU  - Escoubas JM
AUID- ORCID: 0000-0002-6138-1148
AD  - IHPE, CNRS, Ifremer, UPVD, University of Montpellier, Montpellier F-34095, 
      France.
LA  - eng
PT  - Journal Article
DEP - 20220423
PL  - England
TA  - Virus Evol
JT  - Virus evolution
JID - 101664675
PMC - PMC9119428
OTO - NOTNLM
OT  - OsHV-1
OT  - Pacific oyster
OT  - genetic diversity
OT  - herpesvirus
OT  - minor variations
OT  - non-redundant genomes
OT  - phylogeography
OT  - viral spread
EDAT- 2022/05/24 06:00
MHDA- 2022/05/24 06:01
PMCR- 2022/04/23
CRDT- 2022/05/23 03:48
PHST- 2021/07/22 00:00 [received]
PHST- 2022/04/19 00:00 [revised]
PHST- 2022/04/22 00:00 [accepted]
PHST- 2022/05/23 03:48 [entrez]
PHST- 2022/05/24 06:00 [pubmed]
PHST- 2022/05/24 06:01 [medline]
PHST- 2022/04/23 00:00 [pmc-release]
AID - veac039 [pii]
AID - 10.1093/ve/veac039 [doi]
PST - epublish
SO  - Virus Evol. 2022 Apr 23;8(1):veac039. doi: 10.1093/ve/veac039. eCollection 2022.