PMID- 35602246
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2688-6146 (Electronic)
IS  - 2688-6146 (Linking)
VI  - 3
IP  - 2
DP  - 2022 May
TI  - Fibrin clot characteristics and anticoagulant response in a SARS-CoV-2-infected 
      endothelial model.
PG  - 326-334
LID - 10.1002/jha2.407 [doi]
AB  - Coronavirus disease 2019 (COVID-19) patients have increased thrombosis risk. With 
      increasing age, there is an increase in COVID-19 severity. Additionally, adults 
      with a history of vasculopathy have the highest thrombotic risk in COVID-19. The 
      mechanisms of these clinical differences in risk remain unclear. Human umbilical 
      vein endothelial cells (HUVECs) were infected with SARS-CoV-2, influenza 
      A/Singapore/6/86 (H1N1) or mock-infected prior to incubation with plasma from 
      healthy children, healthy adults or vasculopathic adults. Fibrin on surface of 
      cells was observed using scanning electron microscopy, and fibrin characteristics 
      were quantified. This experiment was repeated in the presence of bivalirudin, 
      defibrotide, low-molecular-weight-heparin (LMWH) and unfractionated heparin 
      (UFH). Fibrin formed on SARS-CoV-2 infected HUVECs was densely packed and 
      contained more fibrin compared to mock-infected cells. Fibrin generated from 
      child plasma was the thicker than fibrin generated in vasculopathic adult plasma 
      (p = 0.0165). Clot formation was inhibited by LMWH (0.5 U/ml) and UFH (0.1-0.7 
      U/ml). We show that in the context of the SARS-CoV-2 infection on an endothelial 
      culture, plasma from vasculopathic adults produces fibrin clots with thinner 
      fibrin, indicating that the plasma coagulation system may play a role in 
      determining the thrombotic outcome of SARS-CoV-2 infection. Heparinoid 
      anticoagulants were most effective at preventing clot formation.
CI  - (c) 2022 The Authors. eJHaem published by British Society for Haematology and John 
      Wiley & Sons Ltd.
FAU - McCafferty, Conor
AU  - McCafferty C
AD  - Department of Paediatrics The University of Melbourne Melbourne Victoria 
      Australia.
AD  - Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
FAU - Lee, Leo
AU  - Lee L
AD  - Department of Microbiology and Immunology The Peter Doherty Institute for 
      Infection and Immunity The University of Melbourne Melbourne Victoria Australia.
FAU - Cai, Tengyi
AU  - Cai T
AD  - Department of Paediatrics The University of Melbourne Melbourne Victoria 
      Australia.
AD  - Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
FAU - Praporski, Slavica
AU  - Praporski S
AD  - Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
FAU - Stolper, Julian
AU  - Stolper J
AD  - Heart Regeneration Murdoch Children's Research Institute Melbourne Victoria 
      Australia.
FAU - Karlaftis, Vasiliki
AU  - Karlaftis V
AD  - Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
FAU - Attard, Chantal
AU  - Attard C
AD  - Department of Paediatrics The University of Melbourne Melbourne Victoria 
      Australia.
AD  - Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
FAU - Myint, David
AU  - Myint D
AD  - TA Scientific Pty. Ltd. Taren Point Sydney New South Wales Australia.
FAU - Carey, Leeanne M
AU  - Carey LM
AD  - Department of Occupational Therapy Social Work and Social Policy La Trobe 
      University Melbourne Victoria Australia.
AD  - Neurorehabilitation and Recovery Florey Institute of Neuroscience and Mental 
      Health University of Melbourne Melbourne Victoria Australia.
FAU - Howells, David W
AU  - Howells DW
AD  - Tasmanian School of Medicine University of Tasmania Hobart Tasmania Australia.
FAU - Donnan, Geoffrey A
AU  - Donnan GA
AD  - Melbourne Brain Centre Royal Melbourne Hospital and University of Melbourne 
      Melbourne Victoria Australia.
FAU - Davis, Stephen
AU  - Davis S
AD  - Melbourne Brain Centre Royal Melbourne Hospital and University of Melbourne 
      Melbourne Victoria Australia.
FAU - Ma, Henry
AU  - Ma H
AD  - Department of Neurology and Stroke Monash Health Hospital Melbourne Victoria 
      Australia.
FAU - Crewther, Sheila
AU  - Crewther S
AD  - Department of Psychology and Counselling La Trobe University Melbourne Victoria 
      Australia.
FAU - Nguyen, Vinh A
AU  - Nguyen VA
AD  - Department of Psychology and Counselling La Trobe University Melbourne Victoria 
      Australia.
FAU - Van Den Helm, Suelyn
AU  - Van Den Helm S
AD  - Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
FAU - Letunica, Natasha
AU  - Letunica N
AD  - Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
FAU - Swaney, Ella
AU  - Swaney E
AD  - Department of Paediatrics The University of Melbourne Melbourne Victoria 
      Australia.
AD  - Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
FAU - Elliott, David
AU  - Elliott D
AD  - Heart Regeneration Murdoch Children's Research Institute Melbourne Victoria 
      Australia.
FAU - Subbarao, Kanta
AU  - Subbarao K
AD  - Department of Microbiology and Immunology The Peter Doherty Institute for 
      Infection and Immunity The University of Melbourne Melbourne Victoria Australia.
AD  - WHO Collaborating Centre for Reference and Research on Influenza The Peter 
      Doherty Institute for Infection and Immunity Melbourne Victoria Australia.
FAU - Ignjatovic, Vera
AU  - Ignjatovic V
AD  - Department of Paediatrics The University of Melbourne Melbourne Victoria 
      Australia.
AD  - Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
FAU - Monagle, Paul
AU  - Monagle P
AD  - Department of Paediatrics The University of Melbourne Melbourne Victoria 
      Australia.
AD  - Haematology Murdoch Children's Research Institute Melbourne Victoria Australia.
AD  - Department of Clinical Haematology The Royal Children's Hospital Melbourne 
      Victoria Australia.
AD  - Kids Cancer Centre Sydney Children's Hospital Randwick New South Wales Australia.
LA  - eng
PT  - Journal Article
DEP - 20220322
PL  - United States
TA  - EJHaem
JT  - EJHaem
JID - 101761942
PMC - PMC9110985
OTO - NOTNLM
OT  - anticoagulation
OT  - blood coagulation
OT  - cell culture
OT  - electron microscopy
OT  - viruses
COIS- The authors declare that there is no conflict of interest that could be perceived 
      as prejudicing the impartiality of the research reported.
EDAT- 2022/05/24 06:00
MHDA- 2022/05/24 06:01
PMCR- 2022/03/22
CRDT- 2022/05/23 04:23
PHST- 2022/01/10 00:00 [received]
PHST- 2022/02/16 00:00 [revised]
PHST- 2022/02/18 00:00 [accepted]
PHST- 2022/05/24 06:00 [pubmed]
PHST- 2022/05/24 06:01 [medline]
PHST- 2022/05/23 04:23 [entrez]
PHST- 2022/03/22 00:00 [pmc-release]
AID - JHA2407 [pii]
AID - 10.1002/jha2.407 [doi]
PST - epublish
SO  - EJHaem. 2022 Mar 22;3(2):326-334. doi: 10.1002/jha2.407. eCollection 2022 May.