PMID- 3560261
OWN - NLM
STAT- MEDLINE
DCOM- 19870507
LR  - 20130528
IS  - 0098-4108 (Print)
IS  - 0098-4108 (Linking)
VI  - 20
IP  - 4
DP  - 1987
TI  - Effects of the fungicide methyl-benzimidazol-2-yl carbamate (MBC) on mouse germ 
      cells as determined by flow cytometry.
PG  - 387-99
AB  - Dual-parameter (DNA, RNA) flow cytometry (FCM) measurements were made on 
      testicular and epididymal sperm cells isolated from mice exposed by oral gavage 
      to 0, 250, 500, or 1000 mg/kg X 5 d of the fungicide methylbenzimidazol-2-yl 
      carbamate (MBC), which is known to bind with tubulin subunits and inhibit 
      polymerization and microtubule formation. Effects of exposure to MBC were 
      measured at 7, 24, and 39 d posttreatment. MBC had no effect on body weights, but 
      testis weights and sperm parameters were altered, with few exceptions, only at 
      the highest exposure level. Testis weights were reduced by about 25% at 7 and 24 
      d after exposure; recovery was observed by 39 d after treatment. FCM measurements 
      of testicular cells showed relative percentages of certain testicular populations 
      (round, elongating, and elongated spermatids) were different from the control 
      pattern 7 and 24 d after treatment. The mean percent of cauda epididymal sperm 
      head morphology abnormalities and the susceptibility of the nuclear DNA to 
      denaturation were both elevated at 7, 24, and 39 d after exposure to 1000 mg/kg. 
      The level of denaturation was determined by FCM measurements of the metachromatic 
      shift in acridine orange (AO) stained sperm nuclei from green (native DNA) to red 
      (single-stranded DNA) fluorescence and quantitated by the expression alpha 
      t[red/(red + green] fluorescence. These data demonstrate that spermatogenesis is 
      sensitive to high-dose MBC exposure resulting in an altered ratio of testicular 
      cell types present, abnormal sperm head morphology, and an altered sperm 
      chromatin structure.
FAU - Evenson, D P
AU  - Evenson DP
FAU - Janca, F C
AU  - Janca FC
FAU - Jost, L K
AU  - Jost LK
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Toxicol Environ Health
JT  - Journal of toxicology and environmental health
JID - 7513622
RN  - 0 (Benzimidazoles)
RN  - 0 (Carbamates)
RN  - 0 (Fungicides, Industrial)
RN  - 0 (Polymers)
RN  - 0 (Tubulin)
RN  - 9007-49-2 (DNA)
RN  - H75J14AA89 (carbendazim)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Benzimidazoles/*toxicity
MH  - *Carbamates
MH  - DNA/drug effects
MH  - Flow Cytometry
MH  - Fungicides, Industrial/*toxicity
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Microtubules/drug effects
MH  - Nucleic Acid Denaturation/drug effects
MH  - Organ Size/drug effects
MH  - Polymers/metabolism
MH  - Sperm Head/drug effects
MH  - Spermatozoa/*drug effects
MH  - Testis/pathology
MH  - Tubulin/metabolism
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
AID - 10.1080/15287398709530992 [doi]
PST - ppublish
SO  - J Toxicol Environ Health. 1987;20(4):387-99. doi: 10.1080/15287398709530992.