PMID- 35603572
OWN - NLM
STAT- MEDLINE
DCOM- 20220701
LR  - 20220701
IS  - 1897-5631 (Electronic)
IS  - 0239-8508 (Linking)
VI  - 60
IP  - 2
DP  - 2022
TI  - A chronic moderate methionine administration induced hyperhomocysteinemia 
      associated with cardiovascular disease phenotype in the sand rat Psammomys 
      obesus.
PG  - 111-124
LID - 10.5603/FHC.a2022.0013 [doi]
AB  - INTRODUCTION: Cardiovascular diseases were defined as coronary artery, 
      cerebrovascular, or peripheral arterial disease. Hyperhomocysteinemia (Hhcy) is 
      an independent risk factor of cardiovascular diseases, including atherosclerosis. 
      Our previous studies demonstrated the involvement of Hhcy in cardiovascular 
      remodeling in the sand rat Psammomys obesus. MATERIAL AND METHODS: An 
      experimental Hhcy was induced, in the sand rat Psammomys obesus, by a daily 
      intraperitoneal injection of 70 mg/kg of methionine for a total duration of 6 
      months. The impact of Hhcy on the cellular and matrix structures of the heart, 
      aorta and liver was analyzed using histological techniques. Additionally we 
      treatedprimary cultures of aortic smooth muscle cells (SMCs) with high 
      concentration of methionine to investigate the effects of methionine at the 
      cellular level. RESULTS: A moderate Hhcy induced a significant increase in the 
      extracellular matrix components particularly collagens which accumulated in the 
      interstitial and perivascular spaces in the studied organs indicating a 
      developing fibrosis. A liver steatosis was also observed following methionine 
      treatment. Further analysis of the aorta showed that Hhcy also induced vascular 
      alterations including SMCs reorientation and proliferation associated with 
      aneurysm formation. CONCLUSIONS: Our results show for the first time that Hhcy 
      can induce a cardiovascular and liver diseases phenotype in Psammomys obesus, a 
      species previously shown to be a good model for the studies of diabetes and other 
      metabolism-related pathologies.
FAU - Zerrouk, Fouzia
AU  - Zerrouk F
AD  - Laboratory of Cellular and Molecular Biology, Biochemistry and Remodeling of the 
      Extracellular Matrix, Faculty of Biological Sciences, Houari Boumediene 
      University of Science and Technology (USTHB), Bab Ezzouarel, Algiers, Algeria. 
      fouzia.zerrouk@gmail.com.
FAU - Chaouad, Billel
AU  - Chaouad B
AD  - Laboratory of Cellular and Molecular Biology, Biochemistry and Remodeling of the 
      Extracellular Matrix, Faculty of Biological Sciences, Houari Boumediene 
      University of Science and Technology (USTHB), Bab Ezzouarel, Algiers, Algeria.
FAU - Ghoul, Adel
AU  - Ghoul A
AD  - Laboratory of Cellular and Molecular Biology, Biochemistry and Remodeling of the 
      Extracellular Matrix, Faculty of Biological Sciences, Houari Boumediene 
      University of Science and Technology (USTHB), Bab Ezzouarel, Algiers, Algeria.
FAU - Chalour, Naima
AU  - Chalour N
AD  - Laboratory of Physiology of Organisms, Faculty of Biological Sciences, Houari 
      Boumediene University of Science and Technology (USTHB), Bab Ezzouar, Algiers, 
      Algeria.
FAU - Moulahoum, Anissa
AU  - Moulahoum A
AD  - Laboratory of Cellular and Molecular Biology, Biochemistry and Remodeling of the 
      Extracellular Matrix, Faculty of Biological Sciences, Houari Boumediene 
      University of Science and Technology (USTHB), Bab Ezzouarel, Algiers, Algeria.
FAU - Khiari, Zineb
AU  - Khiari Z
AD  - Laboratory of Cellular and Molecular Biology, Biochemistry and Remodeling of the 
      Extracellular Matrix, Faculty of Biological Sciences, Houari Boumediene 
      University of Science and Technology (USTHB), Bab Ezzouarel, Algiers, Algeria.
FAU - Cherifi, Mohamed El Hadi
AU  - Cherifi MEH
AD  - Central Laboratory of Biology, EPH Bologhine Ibn Ziri, Algiers, Algeria.
FAU - Aouichat, Souhila
AU  - Aouichat S
AD  - Laboratory of Physiology of Organisms, Team of Cellular and Molecular 
      Physiopathology, Faculty of Biological Sciences, Houari Boumediene University of 
      Science and Technology (USTHB), Bab Ezzouar, Algiers, Algeria.
FAU - Houali, Karim
AU  - Houali K
AD  - Laboratory of analytic biochemistry research and biotechnology, Faculty of 
      Biological Sciences and Agronomic Sciences, Mouloud Mammeri University, 
      Tizi-Ouzou, Algeria.
FAU - Benazzoug, Yasmina
AU  - Benazzoug Y
AD  - Laboratory of Cellular and Molecular Biology, Biochemistry and Remodeling of the 
      Extracellular Matrix, Faculty of Biological Sciences, Houari Boumediene 
      University of Science and Technology (USTHB), Bab Ezzouarel, Algiers, Algeria.
LA  - eng
PT  - Journal Article
DEP - 20220523
PL  - Poland
TA  - Folia Histochem Cytobiol
JT  - Folia histochemica et cytobiologica
JID - 8502651
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - *Cardiovascular Diseases/chemically induced/complications
MH  - Gerbillinae
MH  - *Hyperhomocysteinemia/chemically induced/complications/pathology
MH  - Methionine
MH  - Phenotype
OTO - NOTNLM
OT  - Cardiovascular diseases
OT  - Hyperhomocysteinemia
OT  - Liver
OT  - Methionine
OT  - Psammomys obesus
OT  - SMCs
EDAT- 2022/05/24 06:00
MHDA- 2022/07/02 06:00
CRDT- 2022/05/23 05:42
PHST- 2021/07/27 00:00 [received]
PHST- 2022/05/18 00:00 [accepted]
PHST- 2022/04/26 00:00 [revised]
PHST- 2022/05/24 06:00 [pubmed]
PHST- 2022/07/02 06:00 [medline]
PHST- 2022/05/23 05:42 [entrez]
AID - VM/OJS/J/85014 [pii]
AID - 10.5603/FHC.a2022.0013 [doi]
PST - ppublish
SO  - Folia Histochem Cytobiol. 2022;60(2):111-124. doi: 10.5603/FHC.a2022.0013. Epub 
      2022 May 23.