PMID- 35606453
OWN - NLM
STAT- MEDLINE
DCOM- 20220628
LR  - 20220628
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 414
IP  - 17
DP  - 2022 Jul
TI  - An aggregation-induced emission immunoassay for broad detection of 
      polychlorinated biphenyls in chicken and crab.
PG  - 4963-4975
LID - 10.1007/s00216-022-04123-2 [doi]
AB  - Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) with 
      multiple variants, which may be harmful to human health by absorption and 
      bioaccumulation. To ensure food safety, it is necessary to develop multi-residue 
      immunoassays for broad recognition of PCBs. In this study, by mimicking the 
      generic core structure of PCBs, three haptens have been designed and synthesized 
      for monoclonal antibody (mAb) generation. A carboxylic acid derivative of PCB80 
      was a hapten that induced a mAb with broad recognition of PCBs. The results of 
      ELISA further identified that the mAb could recognize 11 different kinds of PCBs; 
      half-maximal inhibition concentrations (IC(50)) ranged from 33.12 to 
      476.42 ng/mL. Subsequently, using aggregation-induced emission luminogen (AIEgen) 
      nanobeads as the tracer for the output signal, the IC(50) value of the various 
      PCBs was improved to 6.38-252.1 ng/mL. The limit of detection (LOD) varied from 
      0.32 to 42.15 ng/mL. Recoveries of 76.90-95.74% and intra-assay coefficients of 
      variation of 8.5-14.4% were obtained with spiked chicken and crab meat samples. 
      Matrix interference was eliminated by dilution, and no false-positive and 
      false-negative results were observed. The developed assay provides a simple, 
      broad-spectrum, and sensitive tool for detecting PCBs, with high-throughput 
      possibilities for large-scale screening of PCBs in food.
CI  - (c) 2022. Springer-Verlag GmbH Germany, part of Springer Nature.
FAU - Han, Chang
AU  - Han C
AD  - School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, 
      Jiangsu, China.
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Institute of Food Safety 
      and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
FAU - Wang, Yulong
AU  - Wang Y
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Institute of Food Safety 
      and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
FAU - Liu, Pengyan
AU  - Liu P
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Institute of Food Safety 
      and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
FAU - Li, Pan
AU  - Li P
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Institute of Food Safety 
      and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
FAU - Liu, Beibei
AU  - Liu B
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Institute of Food Safety 
      and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
FAU - Ding, Ning
AU  - Ding N
AD  - Institute of Agricultural Products Quality Inspection and Testing Center, Suqian, 
      223801, China.
FAU - Routledge, Michael N
AU  - Routledge MN
AD  - School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, 
      Jiangsu, China.
AD  - School of Medicine, University of Leeds, Leeds, LS2 9JT, UK.
AD  - Jiangsu Education Department, International Joint Research Laboratory of 
      Intelligent Agriculture and Agri-Products Processing (Jiangsu University), 
      Zhenjiang, 212013, China.
FAU - Liu, Zhengjiang
AU  - Liu Z
AD  - School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 
      212013, Jiangsu, China.
FAU - Zhang, Cunzheng
AU  - Zhang C
AD  - School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, 
      Jiangsu, China. zhcz2003@hotmail.com.
AD  - Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory 
      Cultivation Base of Ministry of Science and Technology, Institute of Food Safety 
      and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. 
      zhcz2003@hotmail.com.
LA  - eng
GR  - BX2021011/Jiangsu Province International Cooperation in Science and Technology 
      project/
GR  - 2019YFC1605901/The National Key Research and Development Program of China/
GR  - S202105/Key Science and Technology Research and Development plan of Social 
      Development/
PT  - Journal Article
DEP - 20220524
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Haptens)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - *Brachyura
MH  - Chickens
MH  - Haptens
MH  - Immunoassay/methods
MH  - *Polychlorinated Biphenyls/analysis
MH  - Seafood/analysis
OTO - NOTNLM
OT  - Broad-recognition monoclonal antibody
OT  - Immunoassay based on aggregation-induced emission nanobeads
OT  - Polychlorinated biphenyls
EDAT- 2022/05/24 06:00
MHDA- 2022/06/29 06:00
CRDT- 2022/05/23 23:20
PHST- 2022/02/28 00:00 [received]
PHST- 2022/05/06 00:00 [accepted]
PHST- 2022/04/16 00:00 [revised]
PHST- 2022/05/24 06:00 [pubmed]
PHST- 2022/06/29 06:00 [medline]
PHST- 2022/05/23 23:20 [entrez]
AID - 10.1007/s00216-022-04123-2 [pii]
AID - 10.1007/s00216-022-04123-2 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2022 Jul;414(17):4963-4975. doi: 10.1007/s00216-022-04123-2. 
      Epub 2022 May 24.