PMID- 35609334
OWN - NLM
STAT- MEDLINE
DCOM- 20220526
LR  - 20220716
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 13
IP  - 5
DP  - 2022 May
TI  - Circular RNA_0001187 participates in the regulation of ulcerative colitis 
      development via upregulating myeloid differentiation factor 88.
PG  - 12863-12875
LID - 10.1080/21655979.2022.2077572 [doi]
AB  - Circular RNA (circRNA) had been confirmed to participate in ulcerative colitis 
      (UC) development. Circular RNA_0001187 (Circ_0001187) had been found to be 
      overexpressed in patients with Crohn disease. Therefore, circ_0001187 might be an 
      important circRNA regulating intestinal inflammatory diseases. However, the role 
      and mechanism of circ_0001187 in UC progression remains unclear. The colonic 
      mucosal tissues were obtained from 23 UC patients and 23 healthy normal controls. 
      Tumor necrosis factor-alpha (TNF-alpha) was used to mimic UC cell model in vitro. Cell 
      function was assessed by cell counting kit 8 assay, EdU assay, flow cytometry, 
      ELISA assay and oxidative stress detection. RNA interaction was confirmed by 
      dual-luciferase reporter assay and RIP assay. Serum exosomes were isolated by 
      ultracentrifugation and identified by transmission electron microscope. 
      Circ_0001187 was overexpressed in UC patients. Circ_0001187 knockdown enhanced 
      the proliferation, while suppressed apoptosis, inflammation and oxidative stress 
      of TNF-alpha-induced FHC cells. Circ_0001187 acted as miR-1236-3p sponge, and the 
      effects of circ_0001187 downregulation on TNF-alpha-induced FHC cell injury were 
      overturned by miR-1236-3p inhibitor. MYD88 was targeted by miR-1236-3p, and 
      circ_0001187 sponged miR-1236-3p to regulate MYD88. MYD88 knockdown alleviated 
      TNF-alpha-induced FHC cell injury, and its upregulation revoked the inhibition effect 
      of miR-1236-3p on TNF-alpha-induced FHC cell injury. High expression of circ_0001187 
      also was observed in the serum exosomes of UC patients. Our data confirmed that 
      circ_0001187 facilitated UC progression through miR-1236-3p/MYD88 axis, which 
      might be a potential treatment and diagnosis biomarker for UC.
FAU - Ouyang, Wei
AU  - Ouyang W
AD  - Department of Oncology, Zhuzhou Hospital Affiliated to Xiangya School of 
      Medicine, Central South University, Zhuzhou City, China.
FAU - Wu, Min
AU  - Wu M
AD  - Department of Emergency, Zhuzhou Hospital Affiliated to Xiangya School of 
      Medicine, Central South University, Zhuzhou City, China.
FAU - Wu, Anshan
AU  - Wu A
AD  - Department of Oncology, Zhuzhou Hospital Affiliated to Xiangya School of 
      Medicine, Central South University, Zhuzhou City, China.
FAU - Xiao, Heng
AU  - Xiao H
AD  - Department of Anorectal, Zhuzhou Hospital Affiliated to Xiangya School of 
      Medicine, Central South University, Zhuzhou City, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (MIRN1236 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (RNA, Circular)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - *Colitis, Ulcerative/genetics
MH  - Humans
MH  - *MicroRNAs/genetics/metabolism
MH  - Myeloid Differentiation Factor 88/genetics/metabolism
MH  - RNA, Circular/genetics
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
PMC - PMC9275921
OTO - NOTNLM
OT  - MYD88
OT  - Ulcerative colitis
OT  - circ_0001187
OT  - miR-1236-3p
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/05/25 06:00
MHDA- 2022/05/27 06:00
PMCR- 2022/05/24
CRDT- 2022/05/24 17:42
PHST- 2022/05/24 17:42 [entrez]
PHST- 2022/05/25 06:00 [pubmed]
PHST- 2022/05/27 06:00 [medline]
PHST- 2022/05/24 00:00 [pmc-release]
AID - 2077572 [pii]
AID - 10.1080/21655979.2022.2077572 [doi]
PST - ppublish
SO  - Bioengineered. 2022 May;13(5):12863-12875. doi: 10.1080/21655979.2022.2077572.