PMID- 35612707
OWN - NLM
STAT- MEDLINE
DCOM- 20220810
LR  - 20220818
IS  - 2190-3948 (Electronic)
IS  - 2190-393X (Print)
IS  - 2190-393X (Linking)
VI  - 12
IP  - 9
DP  - 2022 Sep
TI  - Evaluating nanobiomaterial-induced DNA strand breaks using the alkaline comet 
      assay.
PG  - 2243-2258
LID - 10.1007/s13346-022-01178-7 [doi]
AB  - Due to their unique chemical and physical properties, nanobiomaterials (NBMs) are 
      extensively studied for applications in medicine and drug delivery. Despite these 
      exciting properties, their small sizes also make them susceptible to toxicity. 
      Whilst nanomaterial immunotoxicity and cytotoxicity are studied in great depth, 
      there is still limited data on their potential genotoxicity or ability to cause 
      DNA damage. In the past years, new medical device regulations, which came into 
      place in 2020, were developed, which require the assessment of long-term NBM 
      exposure; therefore, in recent years, increased attention is being paid to 
      genotoxicity screening of these materials. In this article, and through an 
      interlaboratory comparison (ILC) study conducted within the Horizon 2020 REFINE 
      project, we assess five different NBM formulations, each with different uses, 
      namely, a bio-persistent gold nanoparticle (AuNP), an IR-780 dye-loaded liposome 
      which is used in deep tissue imaging (LipImage815), an unloaded PACA polymeric 
      nanoparticle used as a drug delivery system (PACA), and two loaded PACA NBMs, 
      i.e. the cabazitaxel drug-loaded PACA (CBZ-PACA) and the NR668 dye-loaded PACA 
      (NR668 PACA) for their potential to cause DNA strand breaks using the alkaline 
      comet assay and discuss the current state of genotoxicity testing for 
      nanomaterials. We have found through our interlaboratory comparison that the 
      alkaline comet assay can be suitably applied to the pre-clinical assessment of 
      NBMs, as a reproducible and repeatable methodology for assessing NBM-induced DNA 
      damage. Workflow for assessing the applicability of the alkaline comet assay to 
      determine nanobiomaterial (NBM)-induced DNA strand breaks, through an 
      interlaboratory comparison study (ILC).
CI  - (c) 2022. The Author(s).
FAU - Tutty, Melissa Anne
AU  - Tutty MA
AUID- ORCID: 0000-0002-6705-2492
AD  - Nanomedicine and Molecular Imaging Group, Clinical Medicine, Trinity 
      Translational Medicine Institute (TTMI), Trinity College Dublin, St James's 
      Hospital, Dublin 8, Ireland. tuttym@tcd.ie.
AD  - Laboratory for Biological Characterization of Advanced Materials (LBCAM), TTMI, 
      Trinity College Dublin, St James's Hospital, Dublin 8, Ireland. tuttym@tcd.ie.
FAU - Vella, Gabriele
AU  - Vella G
AD  - Nanomedicine and Molecular Imaging Group, Clinical Medicine, Trinity 
      Translational Medicine Institute (TTMI), Trinity College Dublin, St James's 
      Hospital, Dublin 8, Ireland.
AD  - Laboratory for Biological Characterization of Advanced Materials (LBCAM), TTMI, 
      Trinity College Dublin, St James's Hospital, Dublin 8, Ireland.
FAU - Vennemann, Antje
AU  - Vennemann A
AD  - IBE R&D Institute for Lung Health gGmbH, Munster, Germany.
FAU - Wiemann, Martin
AU  - Wiemann M
AD  - IBE R&D Institute for Lung Health gGmbH, Munster, Germany.
FAU - Prina-Mello, Adriele
AU  - Prina-Mello A
AUID- ORCID: 0000-0002-4371-2214
AD  - Nanomedicine and Molecular Imaging Group, Clinical Medicine, Trinity 
      Translational Medicine Institute (TTMI), Trinity College Dublin, St James's 
      Hospital, Dublin 8, Ireland. prinamea@tcd.ie.
AD  - Laboratory for Biological Characterization of Advanced Materials (LBCAM), TTMI, 
      Trinity College Dublin, St James's Hospital, Dublin 8, Ireland. prinamea@tcd.ie.
AD  - Trinity St James's Cancer Institute, Trinity College Dublin, St James's Hospital, 
      Dublin 8, Ireland. prinamea@tcd.ie.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220525
PL  - United States
TA  - Drug Deliv Transl Res
JT  - Drug delivery and translational research
JID - 101540061
RN  - 7440-57-5 (Gold)
RN  - 9007-49-2 (DNA)
SB  - IM
EIN - Drug Deliv Transl Res. 2022 Jun 19;:. PMID: 35718850
MH  - Comet Assay/methods
MH  - DNA
MH  - DNA Damage
MH  - *Gold
MH  - *Metal Nanoparticles/toxicity
PMC - PMC9360128
OTO - NOTNLM
OT  - AuNP
OT  - Comet assay
OT  - DNA damage
OT  - Genotoxicity
OT  - HepG2
OT  - LipImage815
OT  - Liposome
OT  - Nanobiomaterials
OT  - PACA
COIS- The authors declare no competing interests.
EDAT- 2022/05/26 06:00
MHDA- 2022/08/11 06:00
PMCR- 2022/05/25
CRDT- 2022/05/25 11:18
PHST- 2022/05/05 00:00 [accepted]
PHST- 2022/05/26 06:00 [pubmed]
PHST- 2022/08/11 06:00 [medline]
PHST- 2022/05/25 11:18 [entrez]
PHST- 2022/05/25 00:00 [pmc-release]
AID - 10.1007/s13346-022-01178-7 [pii]
AID - 1178 [pii]
AID - 10.1007/s13346-022-01178-7 [doi]
PST - ppublish
SO  - Drug Deliv Transl Res. 2022 Sep;12(9):2243-2258. doi: 10.1007/s13346-022-01178-7. 
      Epub 2022 May 25.