PMID- 35614074
OWN - NLM
STAT- MEDLINE
DCOM- 20220527
LR  - 20220721
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 May 25
TI  - Novel electrochemical PMI marker biosensor based on quantum dot dissolution using 
      a double-label strategy.
PG  - 8815
LID - 10.1038/s41598-022-12444-6 [doi]
LID - 8815
AB  - A novel and facile post-mortem interval (PMI) biosensor was fabricated using a 
      double-label strategy to detect the glyceraldehyde 3-phosphate dehydrogenase 
      (GAPDH) biomarker. A monoclonal anti-GAPDH antibody was immobilized on a surface 
      label containing cadmium selenide quantum dots (CdSe QDs) on a cysteamine 
      graphene oxide (Cys-GO) self-assembled monolayer. Glucose oxidase (GOx) was used 
      as a signal label to conjugate with GAPDH. GAPDH recognition was achieved through 
      the dissolution of the surface-attached CdSe QDs by hydrogen peroxide generated 
      through GAPDH-conjugated GOx-catalyzed beta-glucose oxidation. To enhance 
      sensitivity, a competitive interaction was introduced between free and conjugated 
      GAPDH to the active site of the anti-GAPDH antibody. The electrochemical response 
      due to CdSe dissolution decreased proportionally with the concentration of free 
      GAPDH. Differential pulsed voltammetry was conducted to determine the analytical 
      characteristics of the immunosensor, including the limit of detection, linear 
      dynamic range, target selectivity, system stability, and applicability toward the 
      analysis of real samples.
CI  - (c) 2022. The Author(s).
FAU - Jeong, Bongjin
AU  - Jeong B
AD  - Intelligent Convergence Research Laboratory, Electronics and Telecommunications 
      Research Institute, 34129, Daejeon, Republic of Korea. jbj0919@etri.re.kr.
AD  - Graduate School of Analytical Science and Technology, Chungnam National 
      University, 34134, Daejeon, Republic of Korea. jbj0919@etri.re.kr.
FAU - Akter, Rashida
AU  - Akter R
AD  - Graduate School of Analytical Science and Technology, Chungnam National 
      University, 34134, Daejeon, Republic of Korea.
FAU - Oh, Jeonghyun
AU  - Oh J
AD  - Graduate School of Analytical Science and Technology, Chungnam National 
      University, 34134, Daejeon, Republic of Korea.
FAU - Lee, Dong-Gi
AU  - Lee DG
AD  - Division of Life Science, Korea Basic Science Institute, 34133, Daejeon, Republic 
      of Korea.
FAU - Ahn, Chang-Geun
AU  - Ahn CG
AD  - Intelligent Convergence Research Laboratory, Electronics and Telecommunications 
      Research Institute, 34129, Daejeon, Republic of Korea.
FAU - Choi, Jong-Soon
AU  - Choi JS
AD  - Graduate School of Analytical Science and Technology, Chungnam National 
      University, 34134, Daejeon, Republic of Korea. jschoi@kbsi.re.kr.
AD  - Division of Life Science, Korea Basic Science Institute, 34133, Daejeon, Republic 
      of Korea. jschoi@kbsi.re.kr.
FAU - Rahman, Md Aminur
AU  - Rahman MA
AD  - Graduate School of Analytical Science and Technology, Chungnam National 
      University, 34134, Daejeon, Republic of Korea. marahman@cnu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220525
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers)
RN  - EC 1.1.3.4 (Glucose Oxidase)
SB  - IM
MH  - Biomarkers
MH  - *Biosensing Techniques
MH  - Electrochemical Techniques
MH  - Glucose Oxidase
MH  - Immunoassay
MH  - Limit of Detection
MH  - *Quantum Dots/chemistry
MH  - Solubility
PMC - PMC9130975
COIS- The authors declare no competing interests.
EDAT- 2022/05/26 06:00
MHDA- 2022/05/28 06:00
PMCR- 2022/05/25
CRDT- 2022/05/25 23:16
PHST- 2022/04/14 00:00 [received]
PHST- 2022/05/11 00:00 [accepted]
PHST- 2022/05/25 23:16 [entrez]
PHST- 2022/05/26 06:00 [pubmed]
PHST- 2022/05/28 06:00 [medline]
PHST- 2022/05/25 00:00 [pmc-release]
AID - 10.1038/s41598-022-12444-6 [pii]
AID - 12444 [pii]
AID - 10.1038/s41598-022-12444-6 [doi]
PST - epublish
SO  - Sci Rep. 2022 May 25;12(1):8815. doi: 10.1038/s41598-022-12444-6.