PMID- 35615401
OWN - NLM
STAT- MEDLINE
DCOM- 20220527
LR  - 20220716
IS  - 1875-8630 (Electronic)
IS  - 0278-0240 (Print)
IS  - 0278-0240 (Linking)
VI  - 2022
DP  - 2022
TI  - PCAT19 Regulates the Proliferation and Apoptosis of Lung Cancer Cells by 
      Inhibiting miR-25-3p via Targeting the MAP2K4 Signal Axis.
PG  - 2442094
LID - 10.1155/2022/2442094 [doi]
LID - 2442094
AB  - Both PCAT19 and miR-25-3p have been reported in lung cancer studies, but whether 
      there is a correlation between the two and whether they jointly regulate the 
      progress of lung cancer have not been reported yet. Therefore, this study carried 
      out a further in-depth research. The expression of PCAT19 was detected in lung 
      cancer (LC) tissues and cells by quantitative real-time polymerase chain reaction 
      (qRT-PCR). The effect of PCAT19 on tumor growth was detected in a tumor-bearing 
      model of nude mice. PCAT19-transfected cells were treated with Honokiol and 
      anisomycin. The effects of PCAT19 on proliferation, apoptosis, and cycle of LC 
      cells were investigated by biomolecule experiments. The effects of PCAT19 on the 
      expressions of mitogen-activated protein kinase- (MAPK-) related proteins were 
      evaluated by western blotting. The expression of PCAT19 was decreased in LC 
      tissues and related to patient survival, tumor size, and pathology. In addition, 
      upregulation of PCAT19 hindered LC cell proliferation, miR-25-3p expression, and 
      the activation of extracellular regulated protein kinases (ERK) 1/2, p38, and 
      c-Jun N-terminal kinase (JNK), while facilitating LC cell apoptosis. Furthermore, 
      upregulation of PCAT19 reversed the effects of Honokiol and anisomycin on 
      promoting cell proliferation and inhibiting cell apoptosis. Collectively, our 
      findings show that upregulated PCAT19 suppresses proliferation yet promotes the 
      apoptosis of LC cells through modulating the miR-25-3p/MAP2K4 signaling axis.
CI  - Copyright (c) 2022 Bing Wang et al.
FAU - Wang, Bing
AU  - Wang B
AD  - Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital 
      of Chongqing Medical University, No. 76, Linjiang Road, Yuzhong District, 
      Chongqing 400010, China.
FAU - Yang, Shengrong
AU  - Yang S
AD  - Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital 
      of Chongqing Medical University, No. 76, Linjiang Road, Yuzhong District, 
      Chongqing 400010, China.
FAU - Jia, Yang
AU  - Jia Y
AD  - Department of Plastic Surgery, The Second Hospital of Hebei Medical University, 
      No. 215, West Heping Road, Shijiazhuang, Hebei Province 050000, China.
FAU - Yang, Jianru
AU  - Yang J
AD  - Department of Obstetrics and Gynecology, The Second Affiliated Hospital of 
      Chongqing Medical University, No. 76, Linjiang Road, Yuzhong District, Chongqing 
      400010, China.
FAU - Du, Kun
AU  - Du K
AD  - Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital 
      of Chongqing Medical University, No. 76, Linjiang Road, Yuzhong District, 
      Chongqing 400010, China.
FAU - Luo, Yujie
AU  - Luo Y
AD  - Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital 
      of Chongqing Medical University, No. 76, Linjiang Road, Yuzhong District, 
      Chongqing 400010, China.
FAU - Li, Yunhe
AU  - Li Y
AD  - Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital 
      of Chongqing Medical University, No. 76, Linjiang Road, Yuzhong District, 
      Chongqing 400010, China.
FAU - Wang, Zhenghong
AU  - Wang Z
AD  - Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital 
      of Chongqing Medical University, No. 76, Linjiang Road, Yuzhong District, 
      Chongqing 400010, China.
FAU - Liu, Yi
AU  - Liu Y
AD  - Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital 
      of Chongqing Medical University, No. 76, Linjiang Road, Yuzhong District, 
      Chongqing 400010, China.
FAU - Zhu, Bing
AU  - Zhu B
AUID- ORCID: 0000-0003-3900-4255
AD  - Department of Thoracic and Cardiovascular Surgery, The Second Affiliated Hospital 
      of Chongqing Medical University, No. 76, Linjiang Road, Yuzhong District, 
      Chongqing 400010, China.
LA  - eng
PT  - Journal Article
DEP - 20220516
PL  - United States
TA  - Dis Markers
JT  - Disease markers
JID - 8604127
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Lignans)
RN  - 0 (MIRN25 microRNA, human)
RN  - 0 (MIRN25 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 11513CCO0N (honokiol)
RN  - 6C74YM2NGI (Anisomycin)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - EC 2.7.12.2 (MAP2K4 protein, human)
SB  - IM
MH  - Animals
MH  - Anisomycin
MH  - Apoptosis/genetics
MH  - Biphenyl Compounds
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Lignans
MH  - *Lung Neoplasms/genetics/metabolism/pathology
MH  - *MAP Kinase Kinase 4/metabolism
MH  - Mice
MH  - Mice, Nude
MH  - *MicroRNAs/genetics/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
PMC - PMC9126706
COIS- The authors declare no competing interest.
EDAT- 2022/05/27 06:00
MHDA- 2022/05/28 06:00
PMCR- 2022/05/16
CRDT- 2022/05/26 02:23
PHST- 2021/09/14 00:00 [received]
PHST- 2022/03/03 00:00 [revised]
PHST- 2022/03/17 00:00 [accepted]
PHST- 2022/05/26 02:23 [entrez]
PHST- 2022/05/27 06:00 [pubmed]
PHST- 2022/05/28 06:00 [medline]
PHST- 2022/05/16 00:00 [pmc-release]
AID - 10.1155/2022/2442094 [doi]
PST - epublish
SO  - Dis Markers. 2022 May 16;2022:2442094. doi: 10.1155/2022/2442094. eCollection 
      2022.