PMID- 35616318
OWN - NLM
STAT- MEDLINE
DCOM- 20220923
LR  - 20221024
IS  - 1613-4133 (Electronic)
IS  - 1613-4125 (Linking)
VI  - 66
IP  - 18
DP  - 2022 Sep
TI  - Adipocytes-Derived Exosomal microRNA-1224 Inhibits M2 Macrophage Polarization in 
      Obesity-Induced Adipose Tissue Inflammation via MSI2-Mediated Wnt/beta-Catenin Axis.
PG  - e2100889
LID - 10.1002/mnfr.202100889 [doi]
AB  - SCOPE: Phenotypic switch of macrophage polarization in adipose tissue has been 
      associated with obesity-induced adipose tissue inflammation (OATI). Therefore, 
      this study aims to explore the possible mechanism of adipocytes-derived exosomes 
      (ADEs) carrying microRNA-1224 (miR-1224) in M2 macrophage polarization of OATI. 
      METHODS AND RESULTS: miR-1224-knockout (miR-1224-KO) mice for this study, and 
      isolated primary adipocytes from high-fat diet (HFD) or normal diet (SD)-fed mice 
      are developed. ADEs are extracted and cocultured with bone marrow-derived 
      macrophages (BMDMs). The macrophagic crown-like structures (CLS) and M1 and M2 
      phenotype macrophages in epididymal white adipose tissue (epiWAT) are observed by 
      immunohistochemistry and flow cytometry. The obtained data indicate that miR-1224 
      is highly expressed in adipose tissues and adipocytes of obese mice. miR-1224 
      knockout decreases CLS number and increases M2 macrophages polarization in 
      epiWAT. In addition, miR-1224 can be transferred to BMDMs via ADEs, which 
      targeted musashi RNA binding protein 2 (MSI2) expression and inactivated 
      Wnt/beta-catenin pathway, inhibiting macrophage M2 polarization and promoting 
      inflammatory factor release. CONCLUSION: Exosomal miR-1224 derived by adipocytes 
      targets MSI2 and blocks the Wnt/beta-catenin pathway, which inhibits macrophage M2 
      polarization and promotes inflammatory factor release, ultimately promoting OATI.
CI  - (c) 2022 Wiley-VCH GmbH.
FAU - Zhang, Dongdong
AU  - Zhang D
AD  - Department of Endocrinology and Metabolism, Yantai Affiliated Hospital of Binzhou 
      Medical University, Yantai, 264100, P. R. China.
FAU - Yao, Xiaoyan
AU  - Yao X
AD  - Department of Endocrinology and Metabolism, Yantai Affiliated Hospital of Binzhou 
      Medical University, Yantai, 264100, P. R. China.
FAU - Teng, Yaqin
AU  - Teng Y
AD  - Department of Endocrinology and Metabolism, Yantai Affiliated Hospital of Binzhou 
      Medical University, Yantai, 264100, P. R. China.
FAU - Zhao, Tiantian
AU  - Zhao T
AD  - Department of Endocrinology and Metabolism, Yantai Affiliated Hospital of Binzhou 
      Medical University, Yantai, 264100, P. R. China.
FAU - Lin, Liangyan
AU  - Lin L
AD  - Department of Endocrinology and Metabolism, Yantai Affiliated Hospital of Binzhou 
      Medical University, Yantai, 264100, P. R. China.
FAU - Li, Yuanyuan
AU  - Li Y
AD  - Department of Endocrinology and Metabolism, Yantai Affiliated Hospital of Binzhou 
      Medical University, Yantai, 264100, P. R. China.
FAU - Shang, Hongxia
AU  - Shang H
AD  - Department of Endocrinology and Metabolism, Yantai Affiliated Hospital of Binzhou 
      Medical University, Yantai, 264100, P. R. China.
FAU - Jin, Yongjun
AU  - Jin Y
AUID- ORCID: 0000-0002-7834-8395
AD  - Department of Endocrinology and Metabolism, Yantai Affiliated Hospital of Binzhou 
      Medical University, Yantai, 264100, P. R. China.
FAU - Jin, Qingsong
AU  - Jin Q
AD  - Department of Endocrinology and Metabolism, Yantai Affiliated Hospital of Binzhou 
      Medical University, Yantai, 264100, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220728
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (MIRN1224 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Adipocytes/metabolism
MH  - Adipose Tissue/metabolism
MH  - Animals
MH  - Inflammation/metabolism
MH  - Macrophages/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese
MH  - *MicroRNAs/genetics/metabolism
MH  - Obesity/metabolism
MH  - RNA-Binding Proteins/metabolism
MH  - *beta Catenin/metabolism
OTO - NOTNLM
OT  - M2 polarization
OT  - MSI2
OT  - Wnt/beta-catenin pathway
OT  - adipocytes
OT  - exosomes
OT  - macrophage
OT  - microRNA-1224
OT  - obesity adipose tissue inflammation
EDAT- 2022/05/27 06:00
MHDA- 2022/09/24 06:00
CRDT- 2022/05/26 08:13
PHST- 2022/04/27 00:00 [revised]
PHST- 2021/09/27 00:00 [received]
PHST- 2022/05/27 06:00 [pubmed]
PHST- 2022/09/24 06:00 [medline]
PHST- 2022/05/26 08:13 [entrez]
AID - 10.1002/mnfr.202100889 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2022 Sep;66(18):e2100889. doi: 10.1002/mnfr.202100889. Epub 
      2022 Jul 28.