PMID- 35618759
OWN - NLM
STAT- MEDLINE
DCOM- 20220530
LR  - 20221113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 May 26
TI  - Magnetic exposure using Samarium Cobalt (SmC(O5)) increased proliferation and 
      stemness of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs).
PG  - 8904
LID - 10.1038/s41598-022-12653-z [doi]
LID - 8904
AB  - Despite the extensive reports on the potential hazard of magnetic field (MF) 
      exposures on humans, there are also concurrently reported on the improved 
      proliferative property of stem cells at optimum exposure. However, the effect on 
      mesenchymal stem cells (MSCs) remains unknown. Therefore, we aimed to investigate 
      the impact of induced static MF (SMF) on human umbilical cord-derived mesenchymal 
      stem cells (hUC-MSCs) using Samarium Cobalt (SmCO5). At passage 3, hUC-MSCs 
      (1 x 10(4)) were exposed to 21.6 mT SMF by a direct exposure (DE) showed a 
      significantly higher cell count (p < 0.05) in the growth kinetics assays with the 
      shortest population doubling time relative to indirect exposure and negative 
      control. The DE group was committed into the cell cycle with increased S phase 
      (55.18 +/- 1.38%) and G2/M phase (21.75 +/- 1.38%) relative to the NC group [S-phase 
      (13.54 +/- 2.73%); G2/M phase (8.36 +/- 0.28%)]. Although no significant changes were 
      observed in the immunophenotype, the DE group showed an elevated expression of 
      pluripotency-associated markers (OCT4, SOX2, NANOG, and REX1). These results 
      suggest that the MFs could potentially induce proliferation of MSCs, a promising 
      approach to promote stem cells propagation for clinical therapy and research 
      without compromising the stemness of hUC-MSCs.
CI  - (c) 2022. The Author(s).
FAU - Hamid, Haslinda Abdul
AU  - Hamid HA
AD  - Bio Artificial Organ and Regenerative Medicine Unit, National Defense University 
      of Malaysia, Sungai Besi Camp, 57000, Kuala Lumpur, Malaysia.
FAU - Ramasamy, Rajesh
AU  - Ramasamy R
AD  - Stem Cell & Immunity Research Group, Immunology Laboratory, Department of 
      Pathology, Faculty of Medicine and Health Sciences, University Putra Malaysia, 
      43400, Serdang, Malaysia.
AD  - Department of Dental Radiology, Faculty of Dental Medicine, Airlangga University, 
      Surabaya, 60132, Indonesia.
FAU - Mustafa, Mohd Kamarulzaki
AU  - Mustafa MK
AD  - Department of Physics, Faculty of Applied Sciences and Technology, University Tun 
      Hussein Onn Malaysia, Pagoh Campus, KM1, Jalan Panchor, Hub Pendidikan Tinggi 
      Pagoh, 84600, Muar, Johor, Malaysia.
FAU - Hosseinpour Sarmadi, Vahid
AU  - Hosseinpour Sarmadi V
AD  - Institutes of Regenerative Medicine, Faculty of Advanced Technologies in 
      Medicine, Iran University of Medical Sciences, Tehran, Iran.
AD  - Cellular and Molecular Research Centre, Iran University of Medical Sciences, 
      Tehran, Iran.
FAU - Miskon, Azizi
AU  - Miskon A
AD  - Bio Artificial Organ and Regenerative Medicine Unit, National Defense University 
      of Malaysia, Sungai Besi Camp, 57000, Kuala Lumpur, Malaysia. 
      azizimiskon@upnm.edu.my.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220526
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 3G0H8C9362 (Cobalt)
RN  - 42OD65L39F (Samarium)
RN  - Q5OL32724M (samarium cobalt)
SB  - IM
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cobalt
MH  - Humans
MH  - Magnetic Phenomena
MH  - *Mesenchymal Stem Cells
MH  - Samarium
MH  - *Umbilical Cord
PMC - PMC9135697
COIS- The authors declare no competing interests.
EDAT- 2022/05/27 06:00
MHDA- 2022/05/31 06:00
PMCR- 2022/05/26
CRDT- 2022/05/26 23:20
PHST- 2021/11/16 00:00 [received]
PHST- 2022/05/11 00:00 [accepted]
PHST- 2022/05/26 23:20 [entrez]
PHST- 2022/05/27 06:00 [pubmed]
PHST- 2022/05/31 06:00 [medline]
PHST- 2022/05/26 00:00 [pmc-release]
AID - 10.1038/s41598-022-12653-z [pii]
AID - 12653 [pii]
AID - 10.1038/s41598-022-12653-z [doi]
PST - epublish
SO  - Sci Rep. 2022 May 26;12(1):8904. doi: 10.1038/s41598-022-12653-z.