PMID- 35621593
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2310-2861 (Electronic)
IS  - 2310-2861 (Linking)
VI  - 8
IP  - 5
DP  - 2022 May 10
TI  - Decellularized Periosteum-Derived Hydrogels Promote the Proliferation, Migration 
      and Osteogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cells.
LID - 10.3390/gels8050294 [doi]
LID - 294
AB  - Human umbilical cord mesenchymal stem cells (hUCMSCs) are promising for bone 
      tissue engineering, which have a non-invasive harvesting process, high cell 
      yield, favorable proliferation capacity, and low immunogenicity. However, the 
      osteogenic efficacy of hUCMSCs is relatively lower than that of bone marrow 
      mesenchymal stem cells (BMSCs). Hydrogels from decellularized extracellular 
      matrix (dECM) preserve the biological compositions and functions of natural ECM, 
      which can provide tissue-specific cues to regulate phenotypic expression and cell 
      fate. It is unknown, however, whether hydrogels from periosteum can serve as 
      pro-osteogenic carriers of hUCMSCs. Herein, a decellularized periosteum-derived 
      hydrogel (dPH) was fabricated to reveal the effects of periosteum-specific cues 
      on the bioactivities of hUCMSCs. A widely used non-bone/periosteum-derived ECM 
      hydrogel product, Matrigel, was used as the control group. After 
      decellularization, the absence of nuclei in the histological analysis indicated a 
      successful removal of cellular components, which was also confirmed by DNA 
      content quantification. The storage modulus of dPH increased (from 164.49 +/- 29.92 
      Pa to 855.20 +/- 20.67 Pa) with increasing concentration (from 0.5% to 1%). With a 
      highly porous, fibrous microstructure, dPH had a more hydrophilic surface than 
      Matrigel, of which the water contact angle reduced 62.62 +/- 0.04%. Furthermore, 
      dPH prominently promoted the initial cellular spreading with a significantly 
      higher cell surface area (1.47-fold), cell spreading length (1.45-fold) and 
      proliferation (approximately 1.05-1.13-fold) of hUCMSCs than those of Matrigel. 
      Additionally, dPH was conducive to cell migration, whereas no cells migrated to 
      Matrigel in the Transwell model. Compared with those of the Matrigel group, the 
      osteogenesis-related genes expression levels (runt-related transcription factor 2 
      (RUNX2), alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCN)) 
      and mineralized matrix formation (9.74-fold) of the hUCMSCs significantly 
      increased in the dPH group. Our study indicated that dPH could provide a 
      pro-osteogenic microenvironment for hUCMSCs, thereby revealing a promising 
      application potential to repair bone defects.
FAU - Li, Shuyi
AU  - Li S
AD  - Department of Stomatology, Guangdong Provincial People's Hospital, Guangdong 
      Academy of Medical Science, Guangzhou 510080, China.
AD  - Department of Oral and Maxillofacial Surgery/Pathology, Amsterdam UMC and 
      Academic Center for Dentistry Amsterdam (ACTA), Amsterdam Movement Science, Vrije 
      Universiteit Amsterdam, 1081 LA Amsterdam, The Netherlands.
FAU - Deng, Rongli
AU  - Deng R
AD  - PCFM Laboratory, School of Chemistry and School of Materials Science and 
      Engineering, Sun Yat-sen University, Guangzhou 510006, China.
FAU - Forouzanfar, Tim
AU  - Forouzanfar T
AD  - Department of Oral and Maxillofacial Surgery/Pathology, Amsterdam UMC and 
      Academic Center for Dentistry Amsterdam (ACTA), Amsterdam Movement Science, Vrije 
      Universiteit Amsterdam, 1081 LA Amsterdam, The Netherlands.
FAU - Wu, Gang
AU  - Wu G
AUID- ORCID: 0000-0001-8941-2500
AD  - Department of Oral Cell Biology, Academic Center for Dentistry Amsterdam (ACTA), 
      University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The 
      Netherlands.
FAU - Quan, Daping
AU  - Quan D
AUID- ORCID: 0000-0002-6360-4341
AD  - PCFM Laboratory, School of Chemistry and School of Materials Science and 
      Engineering, Sun Yat-sen University, Guangzhou 510006, China.
FAU - Zhou, Miao
AU  - Zhou M
AD  - Department of Stomatology, Guangdong Provincial People's Hospital, Guangdong 
      Academy of Medical Science, Guangzhou 510080, China.
LA  - eng
GR  - 81671029, 82001007/National Natural Science Foundation of China/
GR  - 201803040008/Guangzhou Science, Technology and Innovation Commission/
GR  - 20201A011103/General Guidance Project of Guangzhou Health and Technology/
GR  - 201908440308/China Scholarship Council/
GR  - 2016YFC1102900/National Major Science and Technology Project of China/
PT  - Journal Article
DEP - 20220510
PL  - Switzerland
TA  - Gels
JT  - Gels (Basel, Switzerland)
JID - 101696925
PMC - PMC9140703
OTO - NOTNLM
OT  - Matrigel
OT  - decellularized extracellular matrix-derived hydrogels
OT  - hUCMSCs
OT  - osteogenic differentiation
OT  - periosteum
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2022/05/28 06:00
MHDA- 2022/05/28 06:01
PMCR- 2022/05/10
CRDT- 2022/05/27 09:53
PHST- 2022/01/25 00:00 [received]
PHST- 2022/04/03 00:00 [revised]
PHST- 2022/04/18 00:00 [accepted]
PHST- 2022/05/27 09:53 [entrez]
PHST- 2022/05/28 06:00 [pubmed]
PHST- 2022/05/28 06:01 [medline]
PHST- 2022/05/10 00:00 [pmc-release]
AID - gels8050294 [pii]
AID - gels-08-00294 [pii]
AID - 10.3390/gels8050294 [doi]
PST - epublish
SO  - Gels. 2022 May 10;8(5):294. doi: 10.3390/gels8050294.