PMID- 35622622
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2305-6304 (Electronic)
IS  - 2305-6304 (Linking)
VI  - 10
IP  - 5
DP  - 2022 Apr 22
TI  - Bisphenol A Coupled with a High-Fat Diet Promotes Hepatosteatosis through 
      Reactive-Oxygen-Species-Induced CD36 Overexpression.
LID - 10.3390/toxics10050208 [doi]
LID - 208
AB  - Bisphenol A (BPA) is an endocrine-disrupting chemical that affects lipid 
      metabolism and contributes to non-alcoholic fatty liver disease (NAFLD). The 
      mechanism of BPA exposure in hepatic lipid accumulation and its potential effect 
      on NAFLD remain unclear. This study investigated the effect of 
      BPA-exposure-induced hepatic lipid deposition on the pathology of NAFLD and its 
      underlying mechanism in vitro and in vivo. BPA increased intracellular reactive 
      oxygen species (ROS) levels, and promoted fatty acid uptake through upregulation 
      of a free fatty acid uptake transporter, cluster of differentiation 36 (CD36), in 
      HUH-7 cells. Additionally, C57BL/6 mice administered a 
      high-fat/high-cholesterol/high-cholic acid diet (HFCCD) and BPA (50 mg/kg body 
      weight) for 8 weeks developed a steatohepatitis-like phenotype, characterized by 
      alpha-smooth muscle actin (alpha-SMA, an indicator of hepatic fibrosis) and cleaved 
      caspase 3 (an indicator of apoptosis) in hepatic tissue; moreover, they had a 
      higher oxidative stress index of 8-hydroxydeoxyguanosine (8-OHdG) in liver tissue 
      compared to the control group. Treatment with ROS scavenger n-acetylcysteine 
      (NAC) ameliorated BPA-mediated HFCCD-induced lipid accumulation and 
      steatohepatitis in the livers of treated mice. Our study indicates that BPA acts 
      synergistically to increase hepatic lipid uptake and promote NAFLD development by 
      stimulating ROS-induced CD36 overexpression.
FAU - Lee, Jyun-Lin
AU  - Lee JL
AD  - Department of Biomedical Engineering and Environmental Sciences, National Tsing 
      Hua University, Hsinchu 300, Taiwan.
FAU - Wang, Yao-Chien
AU  - Wang YC
AD  - Department of Emergency Medicine, Taichung Tzu Chi Hospital, Taichung 427, 
      Taiwan.
FAU - Hsu, Yu-An
AU  - Hsu YA
AD  - School of Chinese Medicine, China Medical University, Taichung 404, Taiwan.
FAU - Chen, Chih-Sheng
AU  - Chen CS
AD  - Division of Chinese Medicine, Asia University Hospital, Taichung 413, Taiwan.
AD  - Department of Food Nutrition and Health Biotechnology, Asia University, Taichung 
      401, Taiwan.
AD  - Department of Chinese Medicine, China Medicine University Hospital, Taichung 404, 
      Taiwan.
FAU - Weng, Rui-Cian
AU  - Weng RC
AD  - Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan 
      University, Taipei 106, Taiwan.
AD  - National Applied Research Laboratories, Taiwan Instrument Research Institute 
      (TIRI), Hsinchu 300, Taiwan.
FAU - Lu, Yen-Pei
AU  - Lu YP
AD  - National Applied Research Laboratories, Taiwan Instrument Research Institute 
      (TIRI), Hsinchu 300, Taiwan.
FAU - Chuang, Chun-Yu
AU  - Chuang CY
AUID- ORCID: 0000-0001-8060-192X
AD  - Department of Biomedical Engineering and Environmental Sciences, National Tsing 
      Hua University, Hsinchu 300, Taiwan.
FAU - Wan, Lei
AU  - Wan L
AD  - School of Chinese Medicine, China Medical University, Taichung 404, Taiwan.
AD  - Department of Obstetrics and Gynecology, China Medical University Hospital, 
      Taichung 404, Taiwan.
AD  - Department of Medical Laboratory Science and Biotechnology, Asia University, 
      Taichung 413, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20220422
PL  - Switzerland
TA  - Toxics
JT  - Toxics
JID - 101639637
PMC - PMC9145332
OTO - NOTNLM
OT  - CD36
OT  - bisphenol A
OT  - endocrine-disrupting chemical
OT  - fatty acid uptake
OT  - non-alcoholic fatty liver disease
OT  - reactive oxygen species
COIS- The authors declare no conflict of interest.
EDAT- 2022/05/28 06:00
MHDA- 2022/05/28 06:01
PMCR- 2022/04/22
CRDT- 2022/05/27 13:03
PHST- 2022/03/08 00:00 [received]
PHST- 2022/04/13 00:00 [revised]
PHST- 2022/04/19 00:00 [accepted]
PHST- 2022/05/27 13:03 [entrez]
PHST- 2022/05/28 06:00 [pubmed]
PHST- 2022/05/28 06:01 [medline]
PHST- 2022/04/22 00:00 [pmc-release]
AID - toxics10050208 [pii]
AID - toxics-10-00208 [pii]
AID - 10.3390/toxics10050208 [doi]
PST - epublish
SO  - Toxics. 2022 Apr 22;10(5):208. doi: 10.3390/toxics10050208.