PMID- 35625295
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2079-6382 (Print)
IS  - 2079-6382 (Electronic)
IS  - 2079-6382 (Linking)
VI  - 11
IP  - 5
DP  - 2022 May 12
TI  - Niclosamide as a Repurposing Drug against Corynebacterium striatum 
      Multidrug-Resistant Infections.
LID - 10.3390/antibiotics11050651 [doi]
LID - 651
AB  - Corynebacterium striatum (C. striatum) is an emerging multidrug-resistant (MDR) 
      pathogen associated with nosocomial infections. In this scenario, we screened the 
      antimicrobial activity of the anthelmintic drugs doramectin, moxidectin, 
      selamectin and niclosamide against 20 C. striatum MDR clinical isolates. Among 
      these, niclosamide was the best performing drug against C. striatum. Niclosamide 
      cytotoxicity was evaluated by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl 
      tetrazolium bromide (MTT) assay on immortalized human keratinocyte cells (HaCaT). 
      After 20 h of treatment, the recorded 50% cytotoxic concentration (CC(50)) was 
      2.56 mug/mL. The antibacterial efficacy was determined via disc diffusion, broth 
      microdilution method and time-killing. Against C. striatum, niclosamide induced a 
      growth inhibitory area of 22 mm and the minimum inhibitory concentration that 
      inhibits 90% of bacteria (MIC(90)) was 0.39 mug/mL, exhibiting bactericidal 
      action. The biofilm biomass eradicating action was investigated through crystal 
      violet (CV), MTT and confocal laser scanning microscopy (CLSM). Niclosamide 
      affected the biofilm viability in a dose-dependent manner and degraded biomass by 
      55 and 49% at 0.39 mug/mL and 0.19 mug/mL. CLSM images confirmed the biofilm 
      biomass degradation, showing a drastic reduction in cell viability. This study 
      could promote the drug-repurposing of the anthelmintic FDA-approved niclosamide 
      as a therapeutic agent to counteract the C. striatum MDR infections.
FAU - Folliero, Veronica
AU  - Folliero V
AD  - Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
FAU - Dell'Annunziata, Federica
AU  - Dell'Annunziata F
AUID- ORCID: 0000-0002-1654-8500
AD  - Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
FAU - Roscetto, Emanuela
AU  - Roscetto E
AUID- ORCID: 0000-0002-8130-1790
AD  - Department of Molecular Medicine and Medical Biotechnology, University of Naples 
      Federico II, 80131 Naples, Italy.
FAU - Cammarota, Marcella
AU  - Cammarota M
AD  - Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
FAU - De Filippis, Anna
AU  - De Filippis A
AUID- ORCID: 0000-0002-0395-7962
AD  - Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
FAU - Schiraldi, Chiara
AU  - Schiraldi C
AUID- ORCID: 0000-0002-1066-843X
AD  - Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
FAU - Catania, Maria Rosaria
AU  - Catania MR
AUID- ORCID: 0000-0002-8765-137X
AD  - Department of Molecular Medicine and Medical Biotechnology, University of Naples 
      Federico II, 80131 Naples, Italy.
FAU - Casolaro, Vincenzo
AU  - Casolaro V
AUID- ORCID: 0000-0001-9810-0488
AD  - Department of Medicine Surgery and Dentistry, University of Salerno, Baronissi, 
      84081 Salerno, Italy.
FAU - Perrella, Alessandro
AU  - Perrella A
AUID- ORCID: 0000-0003-2812-8500
AD  - Division Emerging Infectious Disease and High Contagiousness, Hospital D Cotugno, 
      80131 Naples, Italy.
FAU - Galdiero, Massimiliano
AU  - Galdiero M
AUID- ORCID: 0000-0002-1576-6290
AD  - Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 
      80138 Naples, Italy.
FAU - Franci, Gianluigi
AU  - Franci G
AUID- ORCID: 0000-0003-3321-4331
AD  - Department of Medicine Surgery and Dentistry, University of Salerno, Baronissi, 
      84081 Salerno, Italy.
AD  - Clinical Pathology and Microbiology Unit, San Giovanni di Dio e Ruggi D'Aragona 
      University Hospital, 84126 Salerno, Italy.
LA  - eng
PT  - Journal Article
DEP - 20220512
PL  - Switzerland
TA  - Antibiotics (Basel)
JT  - Antibiotics (Basel, Switzerland)
JID - 101637404
PMC - PMC9137567
OTO - NOTNLM
OT  - Corynebacterium striatum
OT  - drug-repurposing
OT  - multidrug-resistant pathogen
COIS- The authors declare that they have no conflict of interest.
EDAT- 2022/05/29 06:00
MHDA- 2022/05/29 06:01
PMCR- 2022/05/12
CRDT- 2022/05/28 01:05
PHST- 2022/04/20 00:00 [received]
PHST- 2022/05/09 00:00 [revised]
PHST- 2022/05/11 00:00 [accepted]
PHST- 2022/05/28 01:05 [entrez]
PHST- 2022/05/29 06:00 [pubmed]
PHST- 2022/05/29 06:01 [medline]
PHST- 2022/05/12 00:00 [pmc-release]
AID - antibiotics11050651 [pii]
AID - antibiotics-11-00651 [pii]
AID - 10.3390/antibiotics11050651 [doi]
PST - epublish
SO  - Antibiotics (Basel). 2022 May 12;11(5):651. doi: 10.3390/antibiotics11050651.