PMID- 35626230
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2075-4418 (Print)
IS  - 2075-4418 (Electronic)
IS  - 2075-4418 (Linking)
VI  - 12
IP  - 5
DP  - 2022 Apr 25
TI  - A Single-Center Experience on HLA Typing with 11 Loci Next Generation Sequencing 
      in Korean Patients with Hematologic Disease.
LID - 10.3390/diagnostics12051074 [doi]
LID - 1074
AB  - The human leukocyte antigen (HLA) system comprises the most polymorphic genes of 
      the human genome and is famous for its potential pathological roles. To 
      accurately type HLA genes and find HLA-matched donors, which are critical for 
      effective hematopoietic transplantation, HLA typing using next-generation 
      sequencing (NGS) was implemented. We aimed to share the experience of HLA typing 
      using NGS in patients with hematologic malignancies and evaluate its association 
      with hematologic diseases. Data from 211 Korean, non-familial patients diagnosed 
      with a hematologic disease were reviewed, and NGS was performed for 11 HLA loci. 
      Three-field HLA typing with G code was successfully achieved for all loci and the 
      known linkage between HLA-DRB3/4/5 and HLA-DRB1 was fully matched. Therefore, 
      NGS-based HLA typing enables a detailed, high-resolution analysis of the HLA 
      system that can help with the selection of suitable donors. Notably, 
      HLA-DRB1*08:02:01G was significantly associated with myelodysplastic syndrome. 
      Although this result confirms the tendency of some alleles to be associated with 
      hematological disorders, this may not be the case in hematologic malignancies. 
      Nonetheless, NGS-based HLA typing data for HLA-DP, HLA-DQ, and HLA-DRB3/4/5 are 
      still warranted for a better understanding of the corresponding locus.
FAU - Kim, Namsoo
AU  - Kim N
AUID- ORCID: 0000-0002-1098-4782
AD  - Department of Laboratory Medicine, Severance Hospital, Yonsei University College 
      of Medicine, Seoul 03722, Korea.
FAU - Kim, Sinyoung
AU  - Kim S
AD  - Department of Laboratory Medicine, Severance Hospital, Yonsei University College 
      of Medicine, Seoul 03722, Korea.
FAU - Choi, Jong Rak
AU  - Choi JR
AD  - Department of Laboratory Medicine, Severance Hospital, Yonsei University College 
      of Medicine, Seoul 03722, Korea.
FAU - Park, Younhee
AU  - Park Y
AUID- ORCID: 0000-0001-8458-1495
AD  - Department of Laboratory Medicine, Severance Hospital, Yonsei University College 
      of Medicine, Seoul 03722, Korea.
LA  - eng
PT  - Journal Article
DEP - 20220425
PL  - Switzerland
TA  - Diagnostics (Basel)
JT  - Diagnostics (Basel, Switzerland)
JID - 101658402
PMC - PMC9139519
OTO - NOTNLM
OT  - HLA typing
OT  - hematologic malignancy
OT  - human leukocyte antigen
OT  - next generation sequencing
COIS- The authors declare that they have no conflict of interest.
EDAT- 2022/05/29 06:00
MHDA- 2022/05/29 06:01
PMCR- 2022/04/25
CRDT- 2022/05/28 01:10
PHST- 2022/03/16 00:00 [received]
PHST- 2022/04/23 00:00 [revised]
PHST- 2022/04/23 00:00 [accepted]
PHST- 2022/05/28 01:10 [entrez]
PHST- 2022/05/29 06:00 [pubmed]
PHST- 2022/05/29 06:01 [medline]
PHST- 2022/04/25 00:00 [pmc-release]
AID - diagnostics12051074 [pii]
AID - diagnostics-12-01074 [pii]
AID - 10.3390/diagnostics12051074 [doi]
PST - epublish
SO  - Diagnostics (Basel). 2022 Apr 25;12(5):1074. doi: 10.3390/diagnostics12051074.