PMID- 35627055
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2304-8158 (Print)
IS  - 2304-8158 (Electronic)
IS  - 2304-8158 (Linking)
VI  - 11
IP  - 10
DP  - 2022 May 19
TI  - Preparation of Core-Shell Rare Earth-Doped Upconversion Nanomaterials and 
      Simultaneous Detection of Two Pesticides in Food.
LID - 10.3390/foods11101485 [doi]
LID - 1485
AB  - Under the excitation of a 980 nm excitation light, the fluorescence signals of 
      the synthesized core-shell NaYF(4):Yb@NaYF(4):Ho and monolayer NaYF(4):Yb,Tm 
      upconversion nanoparticles (UCNPs) were simultaneously detected at 656 and 696 
      nm, respectively. The two upconversion materials were coupled with 
      anti-clothianidin and anti-imidacloprid monoclonal antibodies by the 
      glutaraldehyde cross-linking method as signal probes. Imidacloprid (IMI) and 
      clothianidin (CLO) could compete with antigen-conjugated amino Fe(3)O(4) magnetic 
      nanomaterials for binding to signaling probes, thus establishing a rapid and 
      sensitive fluorescent immunoassay for the simultaneous detection of IMI and CLO. 
      Under optimal conditions, the limits of detection (LOD, IC(10)) and sensitivity 
      (IC(50)) of IMI and CLO were (0.032, 0.028) and (4.7, 2.1) ng/mL, respectively, 
      and the linear assay ranges were at 0.032-285.75 ng/mL and 0.028-200 ng/mL, 
      respectively. Immunoassay did not cross-react significantly with other analogs. 
      In fruits and vegetables such as apples, oranges, peaches, cucumbers, tomatoes 
      and peppers, the mean recoveries of IMI and CLO ranged from 83.33% to 115.02% 
      with relative standard deviations (RSDs) of 1.9% to 9.2% and 1.2% to 9.0%, 
      respectively. Furthermore, the results of the immunoassay correlate well with the 
      high-performance liquid chromatography method used to detect the actual samples.
FAU - Zhu, Wenbo
AU  - Zhu W
AD  - Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, 
      Tianjin Normal University, Tianjin 300387, China.
FAU - Zhao, Lingyan
AU  - Zhao L
AD  - Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, 
      Tianjin Normal University, Tianjin 300387, China.
FAU - Jin, Jingyi
AU  - Jin J
AD  - Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, 
      Tianjin Normal University, Tianjin 300387, China.
FAU - Song, Yang
AU  - Song Y
AUID- ORCID: 0000-0002-4468-5668
AD  - Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, 
      Tianjin Normal University, Tianjin 300387, China.
AD  - State Key Laboratory of Food Nutrition and Safety, College of Food Science and 
      Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
LA  - eng
PT  - Journal Article
DEP - 20220519
PL  - Switzerland
TA  - Foods
JT  - Foods (Basel, Switzerland)
JID - 101670569
PMC - PMC9142009
OTO - NOTNLM
OT  - clothianidin
OT  - core-shell structure upconversion nanomaterials
OT  - food
OT  - imidacloprid
OT  - upconversion nanomaterials
COIS- The authors declare no conflict of interest.
EDAT- 2022/05/29 06:00
MHDA- 2022/05/29 06:01
PMCR- 2022/05/19
CRDT- 2022/05/28 01:14
PHST- 2022/04/02 00:00 [received]
PHST- 2022/05/17 00:00 [revised]
PHST- 2022/05/17 00:00 [accepted]
PHST- 2022/05/28 01:14 [entrez]
PHST- 2022/05/29 06:00 [pubmed]
PHST- 2022/05/29 06:01 [medline]
PHST- 2022/05/19 00:00 [pmc-release]
AID - foods11101485 [pii]
AID - foods-11-01485 [pii]
AID - 10.3390/foods11101485 [doi]
PST - epublish
SO  - Foods. 2022 May 19;11(10):1485. doi: 10.3390/foods11101485.