PMID- 35630172
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2072-666X (Print)
IS  - 2072-666X (Electronic)
IS  - 2072-666X (Linking)
VI  - 13
IP  - 5
DP  - 2022 Apr 29
TI  - Microfluidic Paper-Based Blood Plasma Separation Device as a Potential Tool for 
      Timely Detection of Protein Biomarkers.
LID - 10.3390/mi13050706 [doi]
LID - 706
AB  - A current challenge regarding microfluidic paper-based analytical devices (microPAD) 
      for blood plasma separation (BPS) and electrochemical immunodetection of protein 
      biomarkers is how to achieve a microPAD that yields enough plasma to retain the 
      biomarker for affinity biosensing in a functionalized electrode system. This 
      paper describes the development of a BPS microPAD to detect and quantify the S100B 
      biomarker from peripheral whole blood. The device uses NaCl functionalized VF2 
      filter paper as a sample collection pad, an MF1 filter paper for plasma 
      retention, and an optimized microfluidic channel geometry. An inverted light 
      microscope, scanning electron microscope (SEM), and image processing software 
      were used for visualizing BPS efficiency. A design of experiments (DOE) assessed 
      the device's efficacy using an S100B ELISA Kit to measure clinically relevant 
      S100B concentrations in plasma. The BPS device obtained 50 muL of plasma from 300 
      muL of whole blood after 3.5 min. The statistical correlation of S100B 
      concentrations obtained using plasma from standard centrifugation and the BPS 
      device was 0.98. The BPS device provides a simple manufacturing protocol, short 
      fabrication time, and is capable of S100B detection using ELISA, making one step 
      towards the integration of technologies aimed at low-cost POC testing of 
      clinically relevant biomarkers.
FAU - Burgos-Florez, Francisco
AU  - Burgos-Florez F
AUID- ORCID: 0000-0002-5381-1398
AD  - Biotechnology Research Group, Universidad del Norte, Barranquilla 081007, 
      Colombia.
AD  - Rational Use of Energy and Preservation of the Environment Group (UREMA), 
      Universidad del Norte, Barranquilla 081007, Colombia.
AD  - Health and Technological Innovation, Universidad Simon Bolivar, Facultad de 
      Ingenierias, Barranquilla 080002, Colombia.
FAU - Rodriguez, Alexander
AU  - Rodriguez A
AUID- ORCID: 0000-0001-6424-7254
AD  - Biotechnology Research Group, Universidad del Norte, Barranquilla 081007, 
      Colombia.
FAU - Cervera, Eliana
AU  - Cervera E
AUID- ORCID: 0000-0002-7098-1620
AD  - Biotechnology Research Group, Universidad del Norte, Barranquilla 081007, 
      Colombia.
FAU - De Avila, Marcio
AU  - De Avila M
AUID- ORCID: 0000-0001-7637-5106
AD  - Biotechnology Research Group, Universidad del Norte, Barranquilla 081007, 
      Colombia.
FAU - Sanjuan, Marco
AU  - Sanjuan M
AD  - Rational Use of Energy and Preservation of the Environment Group (UREMA), 
      Universidad del Norte, Barranquilla 081007, Colombia.
FAU - Villalba, Pedro J
AU  - Villalba PJ
AUID- ORCID: 0000-0003-3015-0299
AD  - Biotechnology Research Group, Universidad del Norte, Barranquilla 081007, 
      Colombia.
LA  - eng
GR  - 757-2016/Colciencias/
GR  - 809-2018/Gobernacion del Atlantico/
PT  - Journal Article
DEP - 20220429
PL  - Switzerland
TA  - Micromachines (Basel)
JT  - Micromachines
JID - 101640903
PMC - PMC9142996
OTO - NOTNLM
OT  - ELISA
OT  - TBI biomarkers
OT  - blood plasma separation
OT  - microfluidics
OT  - paper
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2022/05/29 06:00
MHDA- 2022/05/29 06:01
PMCR- 2022/04/29
CRDT- 2022/05/28 01:31
PHST- 2022/04/02 00:00 [received]
PHST- 2022/04/26 00:00 [revised]
PHST- 2022/04/26 00:00 [accepted]
PHST- 2022/05/28 01:31 [entrez]
PHST- 2022/05/29 06:00 [pubmed]
PHST- 2022/05/29 06:01 [medline]
PHST- 2022/04/29 00:00 [pmc-release]
AID - mi13050706 [pii]
AID - micromachines-13-00706 [pii]
AID - 10.3390/mi13050706 [doi]
PST - epublish
SO  - Micromachines (Basel). 2022 Apr 29;13(5):706. doi: 10.3390/mi13050706.