PMID- 35630356
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 10
IP  - 5
DP  - 2022 Apr 26
TI  - Salmonella Enteritidis GalE Protein Inhibits LPS-Induced NLRP3 Inflammasome 
      Activation.
LID - 10.3390/microorganisms10050911 [doi]
LID - 911
AB  - Microbial infection can trigger the assembly of inflammasomes and promote 
      secretion of cytokines, such as IL-1beta and IL-18. It is well-known that Salmonella 
      modulates the activation of NLRC4 (NLR family CARD domain-containing protein 4) 
      and NLRP3 (NLR family pyrin domain-containing 3) inflammasomes, however the 
      mechanisms whereby Salmonella avoids or delays inflammasome activation remain 
      largely unknown. Therefore, we used Salmonella Enteritidis C50336DeltafliC transposon 
      library to screen for genes involved in modulating inflammasomes activation. The 
      screen revealed the galactose metabolism-related gene galE to be essential for 
      inflammasome activation. Here, we found that inflammasome activation was 
      significantly increased in J774A.1 cells or wild-type bone marrow-derived 
      macrophages (BMDMs) during infection by DeltafliCDeltagalE compared to cells infected 
      with DeltafliC. Importantly, we found that secretion of IL-1beta was 
      Caspase-1-dependent, consistent with canonical NLRP3 inflammasome activation. 
      Furthermore, the virulence of DeltafliCDeltagalE was significantly decreased compared to 
      DeltafliC in a mouse model. Finally, RNA-seq analysis showed that multiple signaling 
      pathways related to the inflammasome were subject to regulation by GalE. Taken 
      together, our results suggest that GalE plays an important role in the regulatory 
      network of Salmonella evasion of inflammasome activation.
FAU - Huang, Tingting
AU  - Huang T
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
AD  - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China.
AD  - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal 
      Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, 
      Yangzhou University, Yangzhou 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety of 
      the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
FAU - Gu, Dan
AU  - Gu D
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
AD  - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China.
AD  - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal 
      Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, 
      Yangzhou University, Yangzhou 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety of 
      the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
FAU - Guo, Yaxin
AU  - Guo Y
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
AD  - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China.
AD  - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal 
      Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, 
      Yangzhou University, Yangzhou 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety of 
      the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
FAU - Li, Ang
AU  - Li A
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
AD  - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China.
AD  - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal 
      Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, 
      Yangzhou University, Yangzhou 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety of 
      the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
FAU - Kang, Xilong
AU  - Kang X
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
AD  - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China.
AD  - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal 
      Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, 
      Yangzhou University, Yangzhou 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety of 
      the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
FAU - Jiao, Xinan
AU  - Jiao X
AUID- ORCID: 0000-0002-2214-3358
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
AD  - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China.
AD  - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal 
      Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, 
      Yangzhou University, Yangzhou 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety of 
      the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
FAU - Pan, Zhiming
AU  - Pan Z
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
AD  - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China.
AD  - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal 
      Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, 
      Yangzhou University, Yangzhou 225009, China.
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety of 
      the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
LA  - eng
GR  - 31972685, 32161143011 and 31902278/National Natural Science Foundation of China/
GR  - BRA2020002/the fifth phase of "333 project" scientific research project in 
      Jiangsu Province/
PT  - Journal Article
DEP - 20220426
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC9145252
OTO - NOTNLM
OT  - GalE
OT  - NLRP3 inflammasome
OT  - RNA-seq
OT  - S. Enteritidis
OT  - virulence
COIS- The authors declare no conflict of interest.
EDAT- 2022/05/29 06:00
MHDA- 2022/05/29 06:01
PMCR- 2022/04/26
CRDT- 2022/05/28 01:32
PHST- 2022/04/08 00:00 [received]
PHST- 2022/04/23 00:00 [revised]
PHST- 2022/04/24 00:00 [accepted]
PHST- 2022/05/28 01:32 [entrez]
PHST- 2022/05/29 06:00 [pubmed]
PHST- 2022/05/29 06:01 [medline]
PHST- 2022/04/26 00:00 [pmc-release]
AID - microorganisms10050911 [pii]
AID - microorganisms-10-00911 [pii]
AID - 10.3390/microorganisms10050911 [doi]
PST - epublish
SO  - Microorganisms. 2022 Apr 26;10(5):911. doi: 10.3390/microorganisms10050911.