PMID- 35630407
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230916
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 10
IP  - 5
DP  - 2022 May 3
TI  - Biocalcifying Potential of Ureolytic Bacteria Isolated from Soil for 
      Biocementation and Material Crack Repair.
LID - 10.3390/microorganisms10050963 [doi]
LID - 963
AB  - Microbially induced calcium carbonate precipitation (MICP) has been highlighted 
      for its application in civil engineering, and in the environmental and 
      geotechnical fields. Ureolytic activity is one of the most promising bacterial 
      mechanisms in terms of inducing calcium carbonate formation. In this study, four 
      bacterial isolates with high-yield urease production capabilities were obtained 
      from two-step screening using a high-buffered urea medium. The highest urease 
      activity and calcium carbonate formation was observed in Lysinibacillus 
      fusiformis 5.1 with 4.40 x 10(3) unit/L of urease and 24.15 mg/mL of calcium 
      carbonate, followed by Lysinibacillus xylanilyticus 4.3 with 3.93 x 10(3) unit/L 
      of urease and 22.85 mg/mL of calcium carbonate. The microstructure of the 
      precipitated crystalline calcium carbonate was observed using scanning electron 
      microscopy. X-ray diffraction analysis confirmed that the main polymorph of the 
      calcium carbonate particle obtained from both isolates was calcite. Examination 
      of the material-crack filling in mortar specimens showed that calcite layers had 
      formed along the crack edges and inside after 10 days, and gradually filled the 
      cracks up to the upper surface. These results showed that these two isolates 
      presented robust characteristics of potential MICP-inducing bacteria for civil 
      engineering and material engineering applications.
FAU - Leeprasert, Laxmi
AU  - Leeprasert L
AD  - Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 
      10900, Thailand.
FAU - Chonudomkul, Duenrut
AU  - Chonudomkul D
AD  - Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 
      10900, Thailand.
AD  - Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand.
FAU - Boonmak, Chanita
AU  - Boonmak C
AD  - Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 
      10900, Thailand.
AD  - Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand.
LA  - eng
GR  - FF(KU)18.64/Kasetsart University Research and Development Institute (KURDI)/
PT  - Journal Article
DEP - 20220503
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC9143465
OTO - NOTNLM
OT  - MICP
OT  - calcite
OT  - urease
OT  - ureolytic bacteria
COIS- The authors declare that there are no conflict of interest.
EDAT- 2022/05/29 06:00
MHDA- 2022/05/29 06:01
PMCR- 2022/05/03
CRDT- 2022/05/28 01:33
PHST- 2022/04/07 00:00 [received]
PHST- 2022/04/30 00:00 [revised]
PHST- 2022/05/01 00:00 [accepted]
PHST- 2022/05/28 01:33 [entrez]
PHST- 2022/05/29 06:00 [pubmed]
PHST- 2022/05/29 06:01 [medline]
PHST- 2022/05/03 00:00 [pmc-release]
AID - microorganisms10050963 [pii]
AID - microorganisms-10-00963 [pii]
AID - 10.3390/microorganisms10050963 [doi]
PST - epublish
SO  - Microorganisms. 2022 May 3;10(5):963. doi: 10.3390/microorganisms10050963.