PMID- 35630427
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 10
IP  - 5
DP  - 2022 May 8
TI  - Optimization of Lipopeptide Biosurfactant Production by Salibacterium sp. 4CTb in 
      Batch Stirred-Tank Bioreactors.
LID - 10.3390/microorganisms10050983 [doi]
LID - 983
AB  - Halophilic microorganisms are potentially capable as platforms to produce 
      low-cost biosurfactants. However, the robustness of bioprocesses is still a 
      challenge and, therefore, it is essential to understand the effects of 
      microbiological culture conditions through bioreactor engineering. Based on a 
      design of experiments (DOE) and a response surface methodology (RSM) tailored and 
      taken from the literature, the present work focuses on the evaluation of a 
      composite central design (CCD) under batch cultures in stirred-tank bioreactors 
      with the halophilic bacteria Salibacterium sp. 4CTb in order to determine the 
      operative conditions that favor mass transfer and optimize the production of a 
      lipopeptide. The results obtained showed profiles highlighting the most favorable 
      culture conditions, which lead to an emulsification index (E(24)%) higher than 
      70%. Moreover, through the behavior of dissolved oxygen (DO), it was possible to 
      experimentally evaluate the higher volumetric coefficient of mass transfer in the 
      presence of lipopeptide (k(L)a = 31 1/h) as a key criterion for the synthesis of 
      the biosurfactant on further cell expansion.
FAU - Marquez-Villa, Jose Martin
AU  - Marquez-Villa JM
AUID- ORCID: 0000-0001-8146-5587
AD  - Department of Industrial Biotechnology, CIATEJ-CONACyT, Zapopan 45019, Mexico.
FAU - Mateos-Diaz, Juan Carlos
AU  - Mateos-Diaz JC
AUID- ORCID: 0000-0002-6723-6654
AD  - Department of Industrial Biotechnology, CIATEJ-CONACyT, Zapopan 45019, Mexico.
FAU - Rodriguez-Gonzalez, Jorge Alberto
AU  - Rodriguez-Gonzalez JA
AD  - Department of Industrial Biotechnology, CIATEJ-CONACyT, Zapopan 45019, Mexico.
FAU - Camacho-Ruiz, Rosa Maria
AU  - Camacho-Ruiz RM
AUID- ORCID: 0000-0001-9264-5655
AD  - Department of Industrial Biotechnology, CIATEJ-CONACyT, Zapopan 45019, Mexico.
LA  - eng
PT  - Journal Article
DEP - 20220508
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC9145298
OTO - NOTNLM
OT  - DOE
OT  - bioreactor
OT  - biosurfactant
OT  - extremophiles
OT  - microbial processes
COIS- The authors state that this research was conducted in the absence of any 
      commercial or financial relationships that could be associated with a potential 
      conflict of interest.
EDAT- 2022/05/29 06:00
MHDA- 2022/05/29 06:01
PMCR- 2022/05/08
CRDT- 2022/05/28 01:33
PHST- 2022/04/01 00:00 [received]
PHST- 2022/04/25 00:00 [revised]
PHST- 2022/04/30 00:00 [accepted]
PHST- 2022/05/28 01:33 [entrez]
PHST- 2022/05/29 06:00 [pubmed]
PHST- 2022/05/29 06:01 [medline]
PHST- 2022/05/08 00:00 [pmc-release]
AID - microorganisms10050983 [pii]
AID - microorganisms-10-00983 [pii]
AID - 10.3390/microorganisms10050983 [doi]
PST - epublish
SO  - Microorganisms. 2022 May 8;10(5):983. doi: 10.3390/microorganisms10050983.