PMID- 35633676
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 13
DP  - 2022
TI  - Virological and Histopathological Findings in Boars Naturally Infected With 
      Porcine Reproductive and Respiratory Syndrome Virus Type 1.
PG  - 874498
LID - 10.3389/fmicb.2022.874498 [doi]
LID - 874498
AB  - Major geographical transmission of porcine reproductive and respiratory syndrome 
      virus (PRRSV) occurs via semen when a boar stud is infected. This happened in 
      Denmark in 2019, providing an opportunity to compare previous experimental PRRSV 
      boar studies with natural PRRSV-1 infection in boars. The aim of this study was 
      to investigate the association between the presence of PRRSV RNA in serum, semen, 
      testicles, and epididymis of boars naturally infected with PRRSV and to describe 
      the histological lesions in the testes and epididymis combined with direct 
      visualisation of PRRSV-infected cells by immunohistochemical staining (IHC). The 
      exact timing of infection of each boar was not determined, but based on serology 
      the boars were divided into two groups: acute and late infections. All boars 
      included were sampled the same day. In this study, 35 boars and 10 healthy boars 
      from another PRRSV-negative boar stud were included as histological controls. 
      PRRSV RNA was found most often in serum (51%) and least frequently in semen (22%) 
      and was more often detected in the reproductive tract in the acute phase of 
      infection (p < 0.0001; RR: 2.58). Mononuclear cells and multinuclear giant cells 
      were present in the adluminal compartment of the testis and epididymis in 
      PRRSV-infected boars, but not in control boars (p < 0.05), which supports the 
      hypothesis that macrophages are involved in the venereal spread of the virus.
CI  - Copyright (c) 2022 Pedersen, Blirup-Plum, Kristensen, Kvisgaard, Skade, Jensen and 
      Larsen.
FAU - Pedersen, Kasper
AU  - Pedersen K
AD  - SEGES Danish Pig Research Centre, Aarhus, Denmark.
AD  - Department of Veterinary and Animal Sciences, University of Copenhagen, 
      Frederiksberg, Denmark.
FAU - Blirup-Plum, Sophie Amalie
AU  - Blirup-Plum SA
AD  - Department of Veterinary and Animal Sciences, University of Copenhagen, 
      Frederiksberg, Denmark.
FAU - Kristensen, Charlotte Sonne
AU  - Kristensen CS
AD  - SEGES Danish Pig Research Centre, Aarhus, Denmark.
FAU - Kvisgaard, Lise Kirstine
AU  - Kvisgaard LK
AD  - Department of Veterinary and Animal Sciences, University of Copenhagen, 
      Frederiksberg, Denmark.
FAU - Skade, Lotte
AU  - Skade L
AD  - SEGES Danish Pig Research Centre, Aarhus, Denmark.
FAU - Jensen, Henrik Elvang
AU  - Jensen HE
AD  - Department of Veterinary and Animal Sciences, University of Copenhagen, 
      Frederiksberg, Denmark.
FAU - Larsen, Lars Erik
AU  - Larsen LE
AD  - Department of Veterinary and Animal Sciences, University of Copenhagen, 
      Frederiksberg, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20220511
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC9130840
OTO - NOTNLM
OT  - RT-PCR
OT  - boar
OT  - immunohistochemistry
OT  - natural infection
OT  - porcine reproductive and respiratory syndrome virus
COIS- KP, CK, and LS worked for SEGES Danish Pig Research Centre at the time of sample 
      collection and during the investigation. The remaining authors declare that the 
      research was conducted in the absence of any commercial or financial 
      relationships that could be construed as a potential conflict of interest.
EDAT- 2022/06/01 06:00
MHDA- 2022/06/01 06:01
PMCR- 2022/05/11
CRDT- 2022/05/31 09:44
PHST- 2022/02/12 00:00 [received]
PHST- 2022/03/28 00:00 [accepted]
PHST- 2022/05/31 09:44 [entrez]
PHST- 2022/06/01 06:00 [pubmed]
PHST- 2022/06/01 06:01 [medline]
PHST- 2022/05/11 00:00 [pmc-release]
AID - 10.3389/fmicb.2022.874498 [doi]
PST - epublish
SO  - Front Microbiol. 2022 May 11;13:874498. doi: 10.3389/fmicb.2022.874498. 
      eCollection 2022.