PMID- 35637527
OWN - NLM
STAT- MEDLINE
DCOM- 20220602
LR  - 20220716
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 18
IP  - 1
DP  - 2022 May 30
TI  - TRIM4-mediated ubiquitination of NSP2 restricts porcine reproductive and 
      respiratory syndrome virus proliferation.
PG  - 208
LID - 10.1186/s12917-022-03309-1 [doi]
LID - 208
AB  - Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious and 
      virulent infectious disease caused by the porcine reproductive and respiratory 
      syndrome virus (PRRSV), which has substantial economic losses in the pig industry 
      worldwide, and PRRSV attenuated vaccines and inactivated vaccines do have 
      limitations in immune protection. The discovery of new antiviral targets has 
      become a new research field. The proteomic studies have shown that the PRRSV NSP2 
      protein interacts with tripartite motif protein 4 (TRIM4), but it was still 
      unknown whether TRIM4 regulates PRRSV infections. In this study, the TRIM4 gene 
      from Marc-145 cells was cloned, and it was proved that TRIM4 overexpression 
      inhibits PRRSV replication, whereas TRIM4 small-interfering-RNA knockdown 
      resulted in increased virus titers. Mechanism investigation indicated that TRIM4 
      inhibits PRRSV replication through ubiquitination and degradation of the NSP2 
      protein. Protease inhibitor MG132 (carbobenzoxy-Leu-Leu-leucinal) attenuated the 
      TRIM4-driven degradation of NSP2. Taken together, TRIM4 impairs PRRSV 
      proliferation via ubiquitination and degradation of NSP2.
CI  - (c) 2022. The Author(s).
FAU - Zhao, Mengmeng
AU  - Zhao M
AD  - School of Life Science and Engineering, Foshan University, No. 33, Guangyun Road, 
      Nanhai District, Foshan, 528000, China.
FAU - Sha, Huiyang
AU  - Sha H
AD  - School of Life Science and Engineering, Foshan University, No. 33, Guangyun Road, 
      Nanhai District, Foshan, 528000, China.
FAU - Zhang, Hang
AU  - Zhang H
AD  - School of Life Science and Engineering, Foshan University, No. 33, Guangyun Road, 
      Nanhai District, Foshan, 528000, China.
FAU - Wang, Ruining
AU  - Wang R
AD  - College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, 
      Zhengzhou, 450046, People's Republic of China. 80882@hnuahe.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220530
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Antiviral Agents)
SB  - IM
MH  - Animals
MH  - Antiviral Agents
MH  - Cell Proliferation
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Proteomics
MH  - Swine
MH  - Ubiquitination
MH  - Virus Replication
PMC - PMC9149334
OTO - NOTNLM
OT  - Degradation
OT  - NSP2
OT  - PRRSV
OT  - TRIM4
OT  - Ubiquitination
COIS- The authors declare that they have no competing interests.
EDAT- 2022/06/01 06:00
MHDA- 2022/06/03 06:00
PMCR- 2022/05/30
CRDT- 2022/05/31 14:09
PHST- 2021/08/18 00:00 [received]
PHST- 2022/05/16 00:00 [accepted]
PHST- 2022/05/31 14:09 [entrez]
PHST- 2022/06/01 06:00 [pubmed]
PHST- 2022/06/03 06:00 [medline]
PHST- 2022/05/30 00:00 [pmc-release]
AID - 10.1186/s12917-022-03309-1 [pii]
AID - 3309 [pii]
AID - 10.1186/s12917-022-03309-1 [doi]
PST - epublish
SO  - BMC Vet Res. 2022 May 30;18(1):208. doi: 10.1186/s12917-022-03309-1.