PMID- 35637975
OWN - NLM
STAT- MEDLINE
DCOM- 20220602
LR  - 20220716
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2022
DP  - 2022
TI  - TREM-1 Modulates Dendritic Cells Maturation and Dendritic Cell-Mediated T-Cell 
      Activation Induced by ox-LDL.
PG  - 3951686
LID - 10.1155/2022/3951686 [doi]
LID - 3951686
AB  - Atherosclerosis is a chronic inflammatory disease. The triggering receptor 
      expressed on myeloid cells-1 (TREM-1) plays a crucial role in inflammatory 
      diseases; recently, it was identified as a major upstream proatherogenic 
      receptor, but its mechanism is still unclear. In this study, we explore the role 
      of TREM-1 on dendritic cells maturation and inflammatory responses induced by 
      ox-LDL and its possible mechanism. Human dendritic cells were differentiated from 
      blood monocytes and treated with ox-LDL. Naive autologous T cells were cocultured 
      with pretreated DCs or treated directly. The expression of TREM-1 and 
      inflammatory factors were evaluated by real-time PCR, western blot, and ELISA 
      methods. And the expression of immune factors to evaluate the DCs maturation and 
      T-cell activation were determined by the FACS. Our study showed that ox-LDL 
      induced TREM-1 expression, DC maturation, and T-cell activation. T cells exposed 
      to ox-LDL-treated DCs produced interferon-gamma and interleukin-17 (IL-17). Blocking 
      TREM-1 suppressed the DC maturation, showing lower expression of CD1a, CD40, 
      CD86, CD83, and HLA-DR, and limited their production of tumor necrosis 
      factor-alpha (TNF-alpha), IL-1beta, IL-6, and monocyte chemoattractant protein-1 
      (MCP-1), meanwhile increased transforming growth factor-beta(TGF-beta) and IL-10 
      production. Ox-LDL induced miR-155, miR-27, Let-7c, and miR-185 expression; 
      however, TREM-1 inhibiting decreased miRNA-155 expression. Furthermore, silencing 
      miRNA-155 restores SOCS1 repression induced by ox-LDL. Experiments with T cells 
      derived from carotid atherosclerotic plaques or healthy individuals showed 
      similar results. Our results uncover a new link between ox-LDL and TREM-1 and may 
      provide insight into this interaction in the context of atherosclerosis.
CI  - Copyright (c) 2022 Yun Kai Wang et al.
FAU - Wang, Yun Kai
AU  - Wang YK
AUID- ORCID: 0000-0001-7219-5458
AD  - Department of Cardiology, Shanghai East Hospital, Tongji University School of 
      Medicine, Shanghai, China.
FAU - Wang, Jie
AU  - Wang J
AD  - Department of Cardiology, Taihe Hospital, Hubei University of Medicine, Shiyan, 
      Hubei, China.
FAU - Hua, Feng
AU  - Hua F
AD  - Department of Respiratory Medicine, Affiliated Huzhou Hospital, Zhejiang 
      University school of Medicine, Huzhou, Zhejiang Province, China.
FAU - Shen, Yun Li
AU  - Shen YL
AD  - Department of Cardiology, Shanghai East Hospital, Tongji University School of 
      Medicine, Shanghai, China.
FAU - Han, Lu
AU  - Han L
AD  - Qingdao University Medical College, Qingdao, China.
FAU - You, Jie Yun
AU  - You JY
AD  - Department of Cardiology, Shanghai East Hospital, Tongji University School of 
      Medicine, Shanghai, China.
FAU - Wei, Wei
AU  - Wei W
AD  - Department of Cardiology, Shanghai East Hospital, Tongji University School of 
      Medicine, Shanghai, China.
FAU - Zhang, Chun Yu
AU  - Zhang CY
AD  - Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai, China.
FAU - Liu, Xiang Dong
AU  - Liu XD
AD  - Department of Emergency, Shanghai East Hospital, Tongji University School of 
      Medicine, Shanghai, China.
FAU - Zhang, Qi
AU  - Zhang Q
AUID- ORCID: 0000-0002-8935-8396
AD  - Department of Cardiology, Shanghai East Hospital, Tongji University School of 
      Medicine, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20220521
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (MIRN155 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (TREM1 protein, human)
RN  - 0 (Triggering Receptor Expressed on Myeloid Cells-1)
RN  - 0 (oxidized low density lipoprotein)
SB  - IM
MH  - *Atherosclerosis/metabolism
MH  - Dendritic Cells
MH  - Humans
MH  - Lipoproteins, LDL
MH  - MicroRNAs/metabolism
MH  - *Triggering Receptor Expressed on Myeloid Cells-1/metabolism
PMC - PMC9148251
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/06/01 06:00
MHDA- 2022/06/03 06:00
PMCR- 2022/05/21
CRDT- 2022/05/31 14:25
PHST- 2021/07/26 00:00 [received]
PHST- 2022/03/21 00:00 [revised]
PHST- 2022/04/05 00:00 [accepted]
PHST- 2022/05/31 14:25 [entrez]
PHST- 2022/06/01 06:00 [pubmed]
PHST- 2022/06/03 06:00 [medline]
PHST- 2022/05/21 00:00 [pmc-release]
AID - 10.1155/2022/3951686 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2022 May 21;2022:3951686. doi: 10.1155/2022/3951686. 
      eCollection 2022.