PMID- 35643541
OWN - NLM
STAT- MEDLINE
DCOM- 20220603
LR  - 20220716
IS  - 1756-3305 (Electronic)
IS  - 1756-3305 (Linking)
VI  - 15
IP  - 1
DP  - 2022 May 28
TI  - Plasmodium manipulates the expression of host long non-coding RNA during red 
      blood cell intracellular infection.
PG  - 182
LID - 10.1186/s13071-022-05298-4 [doi]
LID - 182
AB  - BACKGROUND: Parasites interact with their host through "direct" and/or "indirect" 
      mechanisms. Plasmodium, for example, either mediates direct physical interactions 
      with host factors or triggers the immune system of the host indirectly, leading 
      to changes in infectious outcomes. Long non-coding RNAs (lncRNAs) participate in 
      regulating biological processes, especially host-pathogen interactions. However, 
      research on the role of host lncRNAs during Plasmodium infection is limited. 
      METHODS: A RNA sequencing method (RNA-seq) was used to confirm the differential 
      expression profiles of lncRNAs in Plasmodium yeolii 17XL (P.y17XL)-infected 
      BALB/c mice. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes 
      (KEGG) pathway analyses were performed to elucidate the potential functions of 
      Plasmodium-induced genes. Subsequently, the effect of specific lncRNAs on the 
      modulation of immune-related signaling pathways in malaria was determined by 
      fluorescence-activated cell sorting, western blot and enzyme-linked immunosorbent 
      assay. RESULTS: The data showed that in P.y17XL-infected BALB/c mice, Plasmodium 
      upregulated the expression of 132 lncRNAs and downregulated the expression of 159 
      lncRNAs. Differentially expressed lncRNAs clearly associated with malaria 
      infection were annotated, including four novel dominant lncRNAs: 
      ENMSUSG00000111521.1, XLOC_038009, XLOC_058629 and XLOC_065676. GO and KEGG 
      pathway analyses demonstrated that these four differentially expressed lncRNAs 
      were associated with co-localized/co-expressed protein-coding genes that were 
      totally enriched in malaria and with the transforming growth factor beta (TGF-beta) 
      signaling pathway. Using the models of P.y17XL-infected BALB/c mice, data 
      certified that the level of TGF-beta production and activation of TGF-beta/Smad(2/3) 
      signaling pathway were obviously changed in malaria infection. CONCLUSIONS: These 
      differentially expressed immune-related genes were deemed to have a role in the 
      process of Plasmodium infection in the host via dendritic/T regulatory cells and 
      the TGF-beta/Smad(2/3) signaling pathway. The results of the present study confirmed 
      that Plasmodium infection-induced lncRNA expression is a novel mechanism used by 
      Plasmodium parasites to modify host immune signaling. These results further 
      enhance current understanding of the interaction between Plasmodium and host 
      cells.
CI  - (c) 2022. The Author(s).
FAU - Chen, Guang
AU  - Chen G
AD  - Department of Basic Medical Sciences, Taizhou University, No. 1139 Shifu Road, 
      Jiaojiang District, Taizhou, 318000, China.
FAU - Liu, Shuang-Chun
AU  - Liu SC
AD  - Municipal Hospital Affiliated to Medical School of Taizhou University, No. 381, 
      Zhongshan East Road, Jiaojiang District, Taizhou, 318000, China.
FAU - Fan, Xiao-Yan
AU  - Fan XY
AD  - Department of Basic Medical Sciences, Taizhou University, No. 1139 Shifu Road, 
      Jiaojiang District, Taizhou, 318000, China.
FAU - Jin, Yue-Lei
AU  - Jin YL
AD  - Department of Basic Medical Sciences, Taizhou University, No. 1139 Shifu Road, 
      Jiaojiang District, Taizhou, 318000, China.
FAU - Li, Xin
AU  - Li X
AD  - Department of Basic Medical Sciences, Taizhou University, No. 1139 Shifu Road, 
      Jiaojiang District, Taizhou, 318000, China.
FAU - Du, Yun-Ting
AU  - Du YT
AD  - Department of Laboratory Medicine, Cancer Hospital of China Medical 
      University-Liaoning Cancer Hospital & Institute, No. 44 Xiaoheyan Road, Dadong 
      District, Shenyang, 110042, China. m15840557769@163.com.
LA  - eng
GR  - 81101278/Young Scientists Fund/
GR  - Z2020080/Outstanding youth program of Taizhou university/
PT  - Journal Article
DEP - 20220528
PL  - England
TA  - Parasit Vectors
JT  - Parasites & vectors
JID - 101462774
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Erythrocytes/metabolism
MH  - Mice
MH  - *Plasmodium/genetics/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Transforming Growth Factor beta
PMC - PMC9148527
OTO - NOTNLM
OT  - Immune signaling
OT  - Intracellular infection
OT  - Long non-coding RNA
OT  - Plasmodium
OT  - RBC
COIS- The authors declare that they have no competing interests.
EDAT- 2022/06/02 06:00
MHDA- 2022/06/07 06:00
PMCR- 2022/05/28
CRDT- 2022/06/01 09:32
PHST- 2021/12/20 00:00 [received]
PHST- 2022/04/26 00:00 [accepted]
PHST- 2022/06/01 09:32 [entrez]
PHST- 2022/06/02 06:00 [pubmed]
PHST- 2022/06/07 06:00 [medline]
PHST- 2022/05/28 00:00 [pmc-release]
AID - 10.1186/s13071-022-05298-4 [pii]
AID - 5298 [pii]
AID - 10.1186/s13071-022-05298-4 [doi]
PST - epublish
SO  - Parasit Vectors. 2022 May 28;15(1):182. doi: 10.1186/s13071-022-05298-4.