PMID- 35644412
OWN - NLM
STAT- MEDLINE
DCOM- 20220621
LR  - 20220828
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 417
IP  - 2
DP  - 2022 Aug 15
TI  - LncRNA HOTTIP facilitates osteogenic differentiation in bone marrow mesenchymal 
      stem cells and induces angiogenesis via interacting with TAF15 to stabilize DLX2.
PG  - 113226
LID - S0014-4827(22)00219-1 [pii]
LID - 10.1016/j.yexcr.2022.113226 [doi]
AB  - AIM: The molecular mechanism of differentiation in bone marrow mesenchymal stem 
      cells (BMSCs) preserves to be further elucidated. LncRNA HOTTIP has been proven 
      to accelerate osteogenic differentiation, but the regulation mechanism is still 
      unclear. METHODS: The human BMSCs (hBMSCs) were isolated and identified by the 
      antigen CD29, CD34, CD44, CD45, and CD90 through flow cytometry. The osteogenic 
      state was determined by the ALP Detection Kit and Alizarin red staining. The tube 
      formation was observed under a microscope. HOTTIP expression level, DLX2 and 
      TAF15, Wnt/beta-catenin pathway, and transcriptional markers in osteogenesis and 
      angiogenesis were examined with Western blot and RT-qPCR, respectively. The 
      combination of TAF15 with lncRNA HOTTIP and DLX2 was detected by RNA 
      immunoprecipitation (RIP) and RNA pulldown assays. RESULTS: The outcomes revealed 
      that HOTTIP was noticeably up-regulated accompanied by the osteogenic 
      transcriptional factor in the process of osteoblast differentiation and 
      angiogenesis. Besides, HOTTIP enhanced alkaline phosphatase (ALP) activity, 
      accelerated osteogenic differentiation and angiogenesis along with up-regulation 
      of osteogenic and angiogenic-related gene expression, by interaction with TAF15 
      to stabilize DLX2. CONCLUSION: Taken together, our outcomes reveal that lncRNA 
      HOTTIP accelerated osteogenic differentiation and angiogenesis by interaction 
      with TAF15 to stabilize DLX2.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Zeng, Xiao
AU  - Zeng X
AD  - Department of Pathology and Pathophysiology, School of Basic Medicine, Guizhou 
      Medical University, Guiyang, 550002, Guizhou Province, PR China; Department of 
      Prosthodontics, The Affiliated Stomatology Hospital of Guizhou Medical 
      University, Guiyang, 550002, Guizhou Province, PR China; Guizhou Medical 
      University School of Stomatology, Guiyang, 550002, Guizhou province, PR China.
FAU - Dong, Qiang
AU  - Dong Q
AD  - Department of Prosthodontics, The Affiliated Stomatology Hospital of Guizhou 
      Medical University, Guiyang, 550002, Guizhou Province, PR China; Guizhou Medical 
      University School of Stomatology, Guiyang, 550002, Guizhou province, PR China.
FAU - Liu, Qin
AU  - Liu Q
AD  - Department of Prosthodontics, The Affiliated Stomatology Hospital of Guizhou 
      Medical University, Guiyang, 550002, Guizhou Province, PR China; Guizhou Medical 
      University School of Stomatology, Guiyang, 550002, Guizhou province, PR China.
FAU - Tan, Wen-Jia
AU  - Tan WJ
AD  - Department of Prosthodontics, The Affiliated Stomatology Hospital of Guizhou 
      Medical University, Guiyang, 550002, Guizhou Province, PR China; Guizhou Medical 
      University School of Stomatology, Guiyang, 550002, Guizhou province, PR China.
FAU - Liu, Xing-De
AU  - Liu XD
AD  - Department of Pathology and Pathophysiology, School of Basic Medicine, Guizhou 
      Medical University, Guiyang, 550002, Guizhou Province, PR China; Guizhou 
      University of Traditional Chinese Medicine, Guiyang, 550002, Guizhou Province, PR 
      China. Electronic address: lxd@gmc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220526
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (DLX2 protein, human)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (TAF15 protein, human)
RN  - 0 (TATA-Binding Protein Associated Factors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Bone Marrow Cells/metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Homeodomain Proteins/genetics/metabolism
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - Osteogenesis/genetics
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - *TATA-Binding Protein Associated Factors/metabolism
MH  - Transcription Factors/genetics/metabolism
MH  - Wnt Signaling Pathway
OTO - NOTNLM
OT  - Angiogenesis
OT  - DLX2
OT  - LncRNA HOTTIP
OT  - Osteogenic differentiation
OT  - TAF15
OT  - Wnt/beta-catenin pathway
EDAT- 2022/06/02 06:00
MHDA- 2022/06/22 06:00
CRDT- 2022/06/01 10:34
PHST- 2022/01/05 00:00 [received]
PHST- 2022/04/24 00:00 [revised]
PHST- 2022/05/22 00:00 [accepted]
PHST- 2022/06/02 06:00 [pubmed]
PHST- 2022/06/22 06:00 [medline]
PHST- 2022/06/01 10:34 [entrez]
AID - S0014-4827(22)00219-1 [pii]
AID - 10.1016/j.yexcr.2022.113226 [doi]
PST - ppublish
SO  - Exp Cell Res. 2022 Aug 15;417(2):113226. doi: 10.1016/j.yexcr.2022.113226. Epub 
      2022 May 26.