PMID- 35648252
OWN - NLM
STAT- MEDLINE
DCOM- 20220725
LR  - 20220725
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 49
IP  - 8
DP  - 2022 Aug
TI  - Genome-wide identification and characterization of major latex-like protein genes 
      responsible for crop contamination in Cucurbita pepo.
PG  - 7773-7782
LID - 10.1007/s11033-022-07602-y [doi]
AB  - BACKGROUND: Zucchini plants (Cucurbita pepo) accumulate persistent organic 
      pollutants (POPs) at high concentrations in their aerial parts, and major 
      latex-like proteins (MLPs) play crucial roles in their accumulation. MLPs bind to 
      POPs in root cells, MLP-POP complexes are then translocated into xylem vessels, 
      and POPs are transported to the aerial parts. We previously identified three 
      CpMLP genes (MLP-PG1, MLP-GR1, and MLP-GR3) as transporting factors for POPs; 
      however, other studies have shown that the genomes of several plant species 
      contain more than 10 MLP genes, thus, further MLP genes responsible for POP 
      accumulation may have been overlooked. METHODS AND RESULTS: Here, we investigated 
      the number of CpMLP genes by performing a hidden Markov model search against the 
      C. pepo genome database and characterized their effects on POP accumulation by 
      performing the expression analysis in the organs and in silico structural 
      analysis. The C. pepo genome contained 21 CpMLP genes, and several CpMLP genes, 
      including MLP-PG1 and MLP-GR3, were highly expressed in roots. 3D structural 
      prediction showed that all examined CpMLPs contained a cavity with a hydrophobic 
      region, which facilitated binding to POPs. CONCLUSIONS: The present study 
      provides insights regarding CpMLP genes responsible for POP accumulation.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Fujita, Kentaro
AU  - Fujita K
AD  - Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodaicho, 
      Nada-ku, Kobe, Hyogo, 657-8501, Japan.
FAU - Chitose, Natsumi
AU  - Chitose N
AD  - Faculty of Agriculture, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 
      657-8501, Japan.
FAU - Chujo, Maho
AU  - Chujo M
AD  - Faculty of Agriculture, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 
      657-8501, Japan.
FAU - Komura, Shoya
AU  - Komura S
AD  - Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodaicho, 
      Nada-ku, Kobe, Hyogo, 657-8501, Japan.
FAU - Sonoda, Chihiro
AU  - Sonoda C
AD  - Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodaicho, 
      Nada-ku, Kobe, Hyogo, 657-8501, Japan.
FAU - Yoshida, Minami
AU  - Yoshida M
AD  - Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodaicho, 
      Nada-ku, Kobe, Hyogo, 657-8501, Japan.
FAU - Inui, Hideyuki
AU  - Inui H
AUID- ORCID: 0000-0002-0981-2641
AD  - Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodaicho, 
      Nada-ku, Kobe, Hyogo, 657-8501, Japan. hinui@kobe-u.ac.jp.
AD  - Biosignal Research Center, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, 
      Hyogo, 657-8501, Japan. hinui@kobe-u.ac.jp.
LA  - eng
GR  - SPRING/Japan Science and Technology Agency/
GR  - Grant-in-Aid for Scientific Research A (23241028)/Ministry of Education, Culture, 
      Sports, and Technology of Japan/
PT  - Journal Article
DEP - 20220601
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Latex)
RN  - 0 (Soil Pollutants)
SB  - IM
MH  - Biodegradation, Environmental
MH  - *Cucurbita/genetics
MH  - Latex/analysis/metabolism
MH  - Plant Roots/metabolism
MH  - *Soil Pollutants/analysis
OTO - NOTNLM
OT  - Crop contamination
OT  - Cucurbita pepo
OT  - Major latex-like protein
OT  - Persistent organic pollutants
EDAT- 2022/06/02 06:00
MHDA- 2022/07/26 06:00
CRDT- 2022/06/01 12:18
PHST- 2022/04/16 00:00 [received]
PHST- 2022/05/13 00:00 [accepted]
PHST- 2022/06/02 06:00 [pubmed]
PHST- 2022/07/26 06:00 [medline]
PHST- 2022/06/01 12:18 [entrez]
AID - 10.1007/s11033-022-07602-y [pii]
AID - 10.1007/s11033-022-07602-y [doi]
PST - ppublish
SO  - Mol Biol Rep. 2022 Aug;49(8):7773-7782. doi: 10.1007/s11033-022-07602-y. Epub 
      2022 Jun 1.