PMID- 35654179
OWN - NLM
STAT- MEDLINE
DCOM- 20220628
LR  - 20220628
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 838
IP  - Pt 4
DP  - 2022 Sep 10
TI  - Extraction of biodegradable microplastics from tissues of aquatic organisms.
PG  - 156396
LID - S0048-9697(22)03493-3 [pii]
LID - 10.1016/j.scitotenv.2022.156396 [doi]
AB  - Biodegradable plastics (BPs) have been given high hopes to substitute 
      conventional plastics, but their biodegradation requires strict conditions. BPs 
      can accumulate for a long time in the environment and even derive biodegradable 
      microplastics (BMPs), thus threatening wildlife and ecosystems. However, no 
      efficient method is available for extracting BMPs from organisms' tissues. This 
      study used multi-criteria decision-making (MCDM) methods to comprehensively 
      evaluate and optimize extraction protocols of five BMPs from economic aquatic 
      species. Digestion time, digestion efficiency, mass loss, cost, polymer integrity 
      and size change were selected as evaluating indictors. According to the screening 
      results of MCDM methods, Pepsin+H(2)O(2) was selected as the optimal digestion 
      method of BMPs because of its highest comprehensive score, which has high 
      digestion efficiency (99.56%) and minimum plastic damage. Compared with olive 
      oil, NaI is more suitable for separating BMPs from the digested residues. 
      Furthermore, the combination of Pepsin+H(2)O(2) digestion and NaI density 
      separation was used to extract all five kinds of BMPs from the bivalve, crab, 
      squid, and crayfish tissues, and all the recovery rates exceeded 80%. These 
      results suggest that the optimal protocol is practicable to extract various BMPs 
      from various aquatic organisms.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Yu, Wenyi
AU  - Yu W
AD  - State Key Laboratory of Pollution Control and Resource Reuse, School of the 
      Environment, Nanjing University, Nanjing, Jiangsu 210023, China.
FAU - Chen, Jiaqi
AU  - Chen J
AD  - State Key Laboratory of Pollution Control and Resource Reuse, School of the 
      Environment, Nanjing University, Nanjing, Jiangsu 210023, China.
FAU - Zhang, Shenghu
AU  - Zhang S
AD  - Nanjing Institute of Environmental Science, Ministry of Ecology and Environment, 
      Nanjing, Jiangsu 210042, China.
FAU - Zhao, Yanping
AU  - Zhao Y
AD  - School of Environment, Nanjing Normal University, Nanjing, Jiangsu 210023, China. 
      Electronic address: yanping-zhao@163.com.
FAU - Fang, Mingliang
AU  - Fang M
AD  - School of Civil and Environmental Engineering, Nanyang Technological University, 
      50 Nanyang Avenue, 639798 Singapore, Singapore.
FAU - Deng, Yongfeng
AU  - Deng Y
AD  - School of Environment and Guangdong Key Laboratory of Environmental Pollution and 
      Health, Jinan University, Guangzhou 510632, China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - State Key Laboratory of Pollution Control and Resource Reuse, School of the 
      Environment, Nanjing University, Nanjing, Jiangsu 210023, China. Electronic 
      address: yanzhang@nju.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220530
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Biodegradable Plastics)
RN  - 0 (Microplastics)
RN  - 0 (Plastics)
RN  - 0 (Water Pollutants, Chemical)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 3.4.23.1 (Pepsin A)
SB  - IM
MH  - Aquatic Organisms/metabolism
MH  - *Biodegradable Plastics
MH  - Ecosystem
MH  - Environmental Monitoring/methods
MH  - Hydrogen Peroxide/metabolism
MH  - Microplastics
MH  - Pepsin A/metabolism
MH  - Plastics/metabolism
MH  - *Water Pollutants, Chemical/analysis
OTO - NOTNLM
OT  - Aquatic organisms
OT  - Biodegradable microplastics
OT  - Density separation
OT  - Digestion
OT  - Extraction method
COIS- Declaration of competing interest The authors declare no competing interests.
EDAT- 2022/06/03 06:00
MHDA- 2022/06/29 06:00
CRDT- 2022/06/02 19:24
PHST- 2022/03/26 00:00 [received]
PHST- 2022/05/27 00:00 [revised]
PHST- 2022/05/28 00:00 [accepted]
PHST- 2022/06/03 06:00 [pubmed]
PHST- 2022/06/29 06:00 [medline]
PHST- 2022/06/02 19:24 [entrez]
AID - S0048-9697(22)03493-3 [pii]
AID - 10.1016/j.scitotenv.2022.156396 [doi]
PST - ppublish
SO  - Sci Total Environ. 2022 Sep 10;838(Pt 4):156396. doi: 
      10.1016/j.scitotenv.2022.156396. Epub 2022 May 30.