PMID- 35655921
OWN - NLM
STAT- MEDLINE
DCOM- 20220606
LR  - 20220716
IS  - 2314-7156 (Electronic)
IS  - 2314-8861 (Print)
IS  - 2314-7156 (Linking)
VI  - 2022
DP  - 2022
TI  - Identification of Significant Secreted or Membrane-Located Proteins in Laryngeal 
      Squamous Cell Carcinoma.
PG  - 9089397
LID - 10.1155/2022/9089397 [doi]
LID - 9089397
AB  - BACKGROUND: This study is aimed at investigating the expressions and prognostic 
      values of secreted or membrane-located proteins (SMPs) in laryngeal squamous cell 
      carcinoma (LSCC). The correlations between the expressions of SMPs and immune 
      cells' infiltrations were also investigated. METHODS: The expression data of 
      normal laryngeal and LSCC samples were obtained from the TCGA and GEO datasets. 
      The differentially expressed SMPs were identified, and their prognostic values 
      were analyzed. The biological functions of differentially expressed and 
      worse-survival-related SMPs were explored. LASSO regression, Cox multivariate 
      analysis, and nomogram were used to construct a model to predict the survival. 
      Then, the infiltrations of the 24 immune cell populations were calculated using 
      the GSVA method, and the correlations between the expression of SMPs and the 
      immune infiltration were investigated. RESULTS: 122 samples (12 normal and 120 
      LSCC) of the TCGA database and 114 samples (57 normal and 57 LSCC) of GSE127165 
      were included. We identified that 138 SMPs were significantly upregulated in LSCC 
      samples of both the TCGA and GEO datasets, among which 52 SMPs were significantly 
      correlated with worse survival. GO and KEGG analyses revealed those 52 SMPs 
      significantly participate in tumor microenvironment and immune cells' 
      communication. Nine of 52 SMPs (ABCC5, ATP1B3, CLEC11A, FLNA, FSTL3, MMP1, NME1, 
      OAS3, and PHLDB2) were included in the nomogram to effectively and accurately 
      predict the LSCC patients' survival. The expressions of most SMPs, such as MMP1 
      and FSTL3, were significantly positively correlated with the immune infiltration 
      of LSCC. CONCLUSIONS: In this study, the expression, prognostic values, and 
      correlations with immune infiltration of SMPs were analyzed in LSCC samples. Our 
      analyses identified several significant SMPs differentially expressed between 
      normal laryngeal and LSCC samples, correlated with worse survival, and related to 
      the immune infiltration.
CI  - Copyright (c) 2022 Li Yan et al.
FAU - Yan, Li
AU  - Yan L
AUID- ORCID: 0000-0002-0697-7838
AD  - Department of Radiation Oncology, Eye & ENT Hospital, Fudan University, Shanghai, 
      China.
FAU - Hu, Chunyan
AU  - Hu C
AUID- ORCID: 0000-0001-7679-5450
AD  - Department of Pathology, Eye & ENT Hospital, Fudan University, Shanghai, China.
FAU - Ji, Yangyang
AU  - Ji Y
AUID- ORCID: 0000-0002-2429-1426
AD  - Department of Otolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 
      China.
FAU - Zou, Lifen
AU  - Zou L
AUID- ORCID: 0000-0002-3600-835X
AD  - Department of Radiation Oncology, Eye & ENT Hospital, Fudan University, Shanghai, 
      China.
FAU - Zhao, Yang
AU  - Zhao Y
AUID- ORCID: 0000-0002-8303-3961
AD  - Department of Radiation Oncology, Eye & ENT Hospital, Fudan University, Shanghai, 
      China.
FAU - Zhu, Yi
AU  - Zhu Y
AUID- ORCID: 0000-0002-2222-0106
AD  - Department of Radiation Oncology, Eye & ENT Hospital, Fudan University, Shanghai, 
      China.
FAU - Wang, Xiaoshen
AU  - Wang X
AUID- ORCID: 0000-0003-1446-5485
AD  - Department of Radiation Oncology, Eye & ENT Hospital, Fudan University, Shanghai, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20220523
PL  - Egypt
TA  - J Immunol Res
JT  - Journal of immunology research
JID - 101627166
RN  - 0 (ATP1B3 protein, human)
RN  - 0 (Follistatin-Related Proteins)
RN  - 0 (Fstl3 protein, human)
RN  - 0 (Membrane Proteins)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - *Follistatin-Related Proteins/genetics
MH  - Humans
MH  - *Laryngeal Neoplasms/genetics
MH  - Matrix Metalloproteinase 1/genetics
MH  - Membrane Proteins/genetics
MH  - Sodium-Potassium-Exchanging ATPase/genetics
MH  - *Squamous Cell Carcinoma of Head and Neck/genetics
MH  - Tumor Microenvironment/genetics
PMC - PMC9153386
COIS- The authors declare no conflict of interest.
EDAT- 2022/06/04 06:00
MHDA- 2022/06/07 06:00
PMCR- 2022/05/23
CRDT- 2022/06/03 02:20
PHST- 2022/04/11 00:00 [received]
PHST- 2022/04/24 00:00 [revised]
PHST- 2022/04/27 00:00 [accepted]
PHST- 2022/06/03 02:20 [entrez]
PHST- 2022/06/04 06:00 [pubmed]
PHST- 2022/06/07 06:00 [medline]
PHST- 2022/05/23 00:00 [pmc-release]
AID - 10.1155/2022/9089397 [doi]
PST - epublish
SO  - J Immunol Res. 2022 May 23;2022:9089397. doi: 10.1155/2022/9089397. eCollection 
      2022.