PMID- 35656867
OWN - NLM
STAT- MEDLINE
DCOM- 20220923
LR  - 20221117
IS  - 1554-6578 (Electronic)
IS  - 0022-3069 (Print)
IS  - 0022-3069 (Linking)
VI  - 81
IP  - 10
DP  - 2022 Sep 19
TI  - Inhibiting Matrix Metalloproteinases Protects Evoked Electromyography Amplitudes 
      and Muscle Tension in the Orbicularis Oris Muscle in a Rat Model of Facial Nerve 
      Injury.
PG  - 816-824
LID - 10.1093/jnen/nlac041 [doi]
AB  - Facial nerve injury results in degradation of the neuromuscular junction (NMJ) 
      and blocks neurotransmission between the pre- and postsynaptic structures, which 
      are separated by a synaptic cleft. Matrix metalloproteinases (MMPs), enzymes that 
      degrade and modify the extracellular matrix, play critical roles in regulating 
      NMJ remodeling. We previously demonstrated that MMP1, MMP2, MMP3, MMP7, and MMP9 
      are overexpressed in facial nerve-innervated orbicularis oris muscle after facial 
      nerve injury in a rat model. In the present study, the MMP inhibitor prinomastat 
      was administered to rats after facial nerve injury. The MMP levels, agrin 
      expression, and muscle-specific kinase (MuSK) phosphorylation were evaluated. 
      Variations in evoked electromyography (EEMG) amplitude were also recorded. 
      Compared with the control group, MMP expression in the orbicularis oris after 
      facial nerve injury was significantly reduced in the prinomastat group. 
      Inhibition of MMP expression maintained agrin expression and MuSK 
      phosphorylation; the NMJ morphology was also protected after the injury. 
      Moreover, prinomastat treatment sustained EEMG amplitude and muscle tension after 
      the injury. These findings indicate that inhibiting MMPs can protect the function 
      and morphology of the NMJ and demonstrate the need for protection of the NMJ at 
      early stages after facial nerve injury.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of American 
      Association of Neuropathologists, Inc.
FAU - Wu, Shuang
AU  - Wu S
AD  - From the Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiao 
      Tong University School of Medicine, Shanghai, China.
FAU - Song, Lijun
AU  - Song L
AD  - Hebei North University, Zhangjiakou, Hebei, P.R. China.
FAU - Yu, Meirong
AU  - Yu M
AD  - From the Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiao 
      Tong University School of Medicine, Shanghai, China.
FAU - Gong, Chao
AU  - Gong C
AD  - From the Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiao 
      Tong University School of Medicine, Shanghai, China.
FAU - Chen, Lianhua
AU  - Chen L
AD  - From the Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiao 
      Tong University School of Medicine, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neuropathol Exp Neurol
JT  - Journal of neuropathology and experimental neurology
JID - 2985192R
RN  - 0 (Agrin)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Organic Chemicals)
RN  - 10T6626FRK (prinomastat)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.23 (Matrix Metalloproteinase 7)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Agrin
MH  - Animals
MH  - Electromyography/methods
MH  - Facial Muscles/innervation/metabolism
MH  - *Facial Nerve Injuries/metabolism
MH  - Matrix Metalloproteinase 1
MH  - Matrix Metalloproteinase 2
MH  - Matrix Metalloproteinase 3
MH  - Matrix Metalloproteinase 7
MH  - Matrix Metalloproteinase 9
MH  - Matrix Metalloproteinase Inhibitors
MH  - Muscle Tonus
MH  - Organic Chemicals
MH  - Rats
PMC - PMC9487608
OTO - NOTNLM
OT  - Extracellular matrix metalloproteinases
OT  - Facial nerve injury
OT  - Neuromuscular junction
OT  - Orbicularis oris
OT  - Prinomastat
EDAT- 2022/06/04 06:00
MHDA- 2022/09/24 06:00
PMCR- 2022/06/03
CRDT- 2022/06/03 05:43
PHST- 2022/06/04 06:00 [pubmed]
PHST- 2022/09/24 06:00 [medline]
PHST- 2022/06/03 05:43 [entrez]
PHST- 2022/06/03 00:00 [pmc-release]
AID - 6599859 [pii]
AID - nlac041 [pii]
AID - 10.1093/jnen/nlac041 [doi]
PST - ppublish
SO  - J Neuropathol Exp Neurol. 2022 Sep 19;81(10):816-824. doi: 10.1093/jnen/nlac041.