PMID- 35658561
OWN - NLM
STAT- MEDLINE
DCOM- 20220623
LR  - 20220623
IS  - 1724-6040 (Electronic)
IS  - 0391-3988 (Linking)
VI  - 45
IP  - 7
DP  - 2022 Jul
TI  - Synergistic effect of miR-9 overexpression and electrical induction on 
      differentiation of conjunctiva mesenchymal stem cells into photoreceptor-like 
      cells.
PG  - 623-630
LID - 10.1177/03913988221103285 [doi]
AB  - A variety of genes and materials can induce the differentiation of stem cells. 
      The purpose of this work was to investigate the effect of microRNA-9 (miR-9) 
      overexpression and electrical induction on the photoreceptor differentiation of 
      Conjunctiva Mesenchymal Stem Cells (CJMSCs). In this study, an electroconductive 
      scaffold (silk fibroin polymer (SF) and reduced graphene oxide (rGo) 
      nanoparticles) was fabricated by electrospinning method, and its characteristics 
      such as diameter, graphene distribution, compound, conductivity, and toxicity 
      were evaluated by scanning and transmission electron microscopy (SEM and TEM), 
      FTIR, electrochemical impedance spectroscopy, and MTT assay. The cells were 
      transduced by a lentiviral vector carrying miR-9, then electrical induction was 
      implied on mir-9-CJMSCs, cultivated on the fabricated scaffold, and the 
      expressions of neural and photoreceptor marker genes were evaluated by RT-qPCR. A 
      uniform, smooth appearance with lower diameter, uniform distribution of rGo 
      nanoparticles across the fibers, and lower resistance were shown in SF-rGo 
      fibrous scaffold. After electrical stimulation, lower and higher expression of 
      neural marker genes and photoreceptor marker genes (Rhodopsin, PKC) were 
      documented, respectively. Finally, we proposed that the combinational approach of 
      miR-9 overexpression and electrical induction leads CJMSCs to photoreceptor-like 
      cells.
FAU - Naderi, Mahmood
AU  - Naderi M
AD  - Cell-Based Therapies Research Center, Digestive Diseases Research Institute, 
      Tehran University of Medical Sciences, Tehran, Iran.
FAU - Nadri, Samad
AU  - Nadri S
AUID- ORCID: 0000-0002-1523-3152
AD  - Department of Medical Nanotechnology, Zanjan University of Medical Sciences, 
      Zanjan, Iran.
AD  - Zanjan Metabolic Diseases Research Center, Zanjan University of Medical Sciences, 
      Zanjan, Iran.
AD  - Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of 
      Medical Sciences, Zanjan, Iran.
LA  - eng
PT  - Journal Article
DEP - 20220603
PL  - United States
TA  - Int J Artif Organs
JT  - The International journal of artificial organs
JID - 7802649
RN  - 0 (MicroRNAs)
RN  - 9007-76-5 (Fibroins)
SB  - IM
MH  - Cell Differentiation
MH  - Conjunctiva/metabolism
MH  - *Fibroins/pharmacology
MH  - *Mesenchymal Stem Cells
MH  - *MicroRNAs/genetics/metabolism/pharmacology
MH  - Tissue Engineering/methods
MH  - Tissue Scaffolds/chemistry
OTO - NOTNLM
OT  - Conjunctiva Mesenchymal Stem Cells (CJMSCs)
OT  - differentiation
OT  - electrical induction
OT  - miR-9 overexpression
OT  - photoreceptor-like cells
EDAT- 2022/06/07 06:00
MHDA- 2022/06/24 06:00
CRDT- 2022/06/06 09:35
PHST- 2022/06/07 06:00 [pubmed]
PHST- 2022/06/24 06:00 [medline]
PHST- 2022/06/06 09:35 [entrez]
AID - 10.1177/03913988221103285 [doi]
PST - ppublish
SO  - Int J Artif Organs. 2022 Jul;45(7):623-630. doi: 10.1177/03913988221103285. Epub 
      2022 Jun 3.