PMID- 35660287
OWN - NLM
STAT- MEDLINE
DCOM- 20220712
LR  - 20220712
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 271
DP  - 2022 Aug
TI  - A synthetic toll-like receptor 7 agonist inhibits porcine reproductive and 
      respiratory syndrome virus replication in piglets.
PG  - 109475
LID - S0378-1135(22)00145-6 [pii]
LID - 10.1016/j.vetmic.2022.109475 [doi]
AB  - Toll-like receptor 7 (TLR7) agonists have been shown to exert therapeutic effects 
      against several viruses. However, antiviral potential of TLR7 agonist in 
      inhibiting porcine reproductive and respiratory syndrome virus (PRRSV) infection 
      has not been assessed in vivo. In our previous study, a synthetic TLR7 agonist, 
      SZU101, was confirmed to inhibit PRRSV infection of porcine alveolar macrophages 
      (PAMs). Here, antiviral effects of SZU101 were evaluated in PRRSV-challenged 
      piglets based on assessments of rectal temperature, viremia, gross and 
      microscopic lung lesions, PRRSV-specific antibodies, PRRSV-specific lymphocyte 
      proliferation and serum IFN-beta level. Our results revealed that SZU101 treatment 
      alleviated PRRSV-induced rectal temperature spikes, pulmonary pathologic changes, 
      and serum viral load. Meanwhile, administration of SZU101 led to increased 
      proliferation of PRRSV-specific lymphocytes and serum IFN-beta levels, but did not 
      enhance PRRSV-specific antibody production. These results demonstrate that SZU101 
      has potential as a therapeutic treatment for PRRS.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Huang, Qiuping
AU  - Huang Q
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Yang, Haoyan
AU  - Yang H
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Yang, Dingfan
AU  - Yang D
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Hao, Yuanjie
AU  - Hao Y
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Yu, Shijia
AU  - Yu S
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Guo, Zhongyi
AU  - Guo Z
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Tian, Lingyujia
AU  - Tian L
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Cai, Xuehui
AU  - Cai X
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Huang, Baicheng
AU  - Huang B
AD  - National Research Center for Veterinary Medicine, Luoyang, China.
FAU - Zhang, Angke
AU  - Zhang A
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, 
      China.
FAU - Wang, Gang
AU  - Wang G
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Veterinary Medicine, Shandong Agricultural University, 
      Taian, China; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. 
      Electronic address: wanggang@sdau.edu.cn.
FAU - Du, Taofeng
AU  - Du T
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: 
      taofengdu@nwsuaf.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220523
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antiviral Agents)
RN  - 0 (Toll-Like Receptor 7)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Adjuvants, Immunologic/pharmacology
MH  - Animals
MH  - Antibodies, Viral
MH  - Antiviral Agents/pharmacology
MH  - Interferon-beta/pharmacology
MH  - Macrophages, Alveolar
MH  - *Porcine Reproductive and Respiratory Syndrome/drug therapy
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Swine
MH  - *Swine Diseases
MH  - Toll-Like Receptor 7
MH  - Virus Replication
OTO - NOTNLM
OT  - PRRSV
OT  - TLR7
OT  - Therapy
OT  - Type Ⅰ interferon
EDAT- 2022/06/07 06:00
MHDA- 2022/07/14 06:00
CRDT- 2022/06/06 11:31
PHST- 2022/02/21 00:00 [received]
PHST- 2022/05/07 00:00 [revised]
PHST- 2022/05/20 00:00 [accepted]
PHST- 2022/06/07 06:00 [pubmed]
PHST- 2022/07/14 06:00 [medline]
PHST- 2022/06/06 11:31 [entrez]
AID - S0378-1135(22)00145-6 [pii]
AID - 10.1016/j.vetmic.2022.109475 [doi]
PST - ppublish
SO  - Vet Microbiol. 2022 Aug;271:109475. doi: 10.1016/j.vetmic.2022.109475. Epub 2022 
      May 23.