PMID- 35662241 OWN - NLM STAT- MEDLINE DCOM- 20221006 LR - 20221006 IS - 1098-1101 (Electronic) IS - 0733-2459 (Linking) VI - 37 IP - 5 DP - 2022 Oct TI - Mobilization with high-dose granulocyte colony-stimulating factor alone at 12 mug/kg twice a day in high-risk pediatric patients: A retrospective analysis of the experience in a single center. PG - 420-429 LID - 10.1002/jca.21994 [doi] AB - INTRODUCTION: Mobilization regimes in pediatric patients at high risk for poor mobilization are not standardized across different institutions. We present a retrospective analysis of our experience with a high-dose granulocyte colony-stimulating factor (G-CSF) regime of 12 mug/Kg per body weight (BW) twice a day for 4 days used in high-risk patients. MATERIAL AND METHODS: We report the results of all pediatric patients mobilized with high-dose G-CSF between January 1999 and February 2021 in our center. A successful mobilization was defined as a peripheral blood (PB) CD34(+) cell count of >/=10 CD34(+) cells/mul on the fifth day of mobilization immediately before leukapheresis. A minimum cell yield of >/=2 x 10(6) CD34(+) cells/Kg of BW was required for a successful collection. RESULTS: Of the 262 patients included in the analysis, mobilization failure was found in 27 (10.3%). In a univariate analysis, this was associated with age, weight, baseline diagnosis, and having undergone a previous mobilization cycle, the latter being the only factor that remained significantly associated in a multivariate analysis (P = 0.03). The 54 patients (20.6%) did not reach the minimum required CD34(+) cell yield. 50.4% of the patients reported adverse events (AEs) during the mobilization period, and 23 (9.1%) reported 3 or more concomitant AEs. However, all of them were mild and did not affect the mobilization schedule. CONCLUSIONS: Although most high-risk pediatric patients are successfully mobilized with the high-dose G-CSF regime, this approach does not salvage all of them and significantly increases the presence of AEs in comparison to standard-dose regimes. CI - (c) 2022 Wiley Periodicals LLC. FAU - Iriondo, June AU - Iriondo J AUID- ORCID: 0000-0001-8010-6240 AD - Hematology and Hemotherapy Unit, Hematology and Oncology Department, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. AD - Fundacion para la Investigacion Biomedica Hospital Infantil Universitario, Nino Jesus, Madrid, Spain. FAU - Zubicaray, Josune AU - Zubicaray J AUID- ORCID: 0000-0003-4115-9598 AD - Hematology and Hemotherapy Unit, Hematology and Oncology Department, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. AD - Fundacion para la Investigacion Biomedica Hospital Infantil Universitario, Nino Jesus, Madrid, Spain. FAU - Sebastian, Elena AU - Sebastian E AD - Hematology and Hemotherapy Unit, Hematology and Oncology Department, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. AD - Fundacion para la Investigacion Biomedica Hospital Infantil Universitario, Nino Jesus, Madrid, Spain. FAU - Gonzalez de Pablo, Jesus AU - Gonzalez de Pablo J AD - Hematology and Hemotherapy Unit, Hematology and Oncology Department, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. AD - Fundacion para la Investigacion Biomedica Hospital Infantil Universitario, Nino Jesus, Madrid, Spain. FAU - Gonzalez-Vicent, Marta AU - Gonzalez-Vicent M AD - Fundacion para la Investigacion Biomedica Hospital Infantil Universitario, Nino Jesus, Madrid, Spain. AD - Hematopoietic Stem Cell Transplantation Unit, Pediatric Hematology and Oncology department, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. FAU - Molina, Blanca AU - Molina B AD - Fundacion para la Investigacion Biomedica Hospital Infantil Universitario, Nino Jesus, Madrid, Spain. AD - Hematopoietic Stem Cell Transplantation Unit, Pediatric Hematology and Oncology department, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. FAU - Lopez-Torija, Ivan AU - Lopez-Torija I AD - Fundacion para la Investigacion Biomedica Hospital Infantil Universitario, Nino Jesus, Madrid, Spain. AD - Hematopoietic Stem Cell Transplantation Unit, Pediatric Hematology and Oncology department, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. FAU - Castillo, Ana AU - Castillo A AD - Fundacion para la Investigacion Biomedica Hospital Infantil Universitario, Nino Jesus, Madrid, Spain. AD - Hematology and Oncology Laboratory Unit, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. FAU - Ramirez, Manuel AU - Ramirez M AD - Fundacion para la Investigacion Biomedica Hospital Infantil Universitario, Nino Jesus, Madrid, Spain. AD - Hematology and Oncology Laboratory Unit, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. FAU - Madero, Luis AU - Madero L AD - Hematology and Hemotherapy Unit, Hematology and Oncology Department, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. AD - Fundacion para la Investigacion Biomedica Hospital Infantil Universitario, Nino Jesus, Madrid, Spain. AD - Hematopoietic Stem Cell Transplantation Unit, Pediatric Hematology and Oncology department, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. AD - Hematology and Oncology Laboratory Unit, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. FAU - Diaz, Miguel Angel AU - Diaz MA AD - Fundacion para la Investigacion Biomedica Hospital Infantil Universitario, Nino Jesus, Madrid, Spain. AD - Hematopoietic Stem Cell Transplantation Unit, Pediatric Hematology and Oncology department, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. FAU - Sevilla, Julian AU - Sevilla J AD - Hematology and Hemotherapy Unit, Hematology and Oncology Department, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. AD - Fundacion para la Investigacion Biomedica Hospital Infantil Universitario, Nino Jesus, Madrid, Spain. LA - eng PT - Journal Article DEP - 20220604 PL - United States TA - J Clin Apher JT - Journal of clinical apheresis JID - 8216305 RN - 0 (Antigens, CD34) RN - 143011-72-7 (Granulocyte Colony-Stimulating Factor) SB - IM MH - Antigens, CD34/analysis MH - Child MH - Granulocyte Colony-Stimulating Factor/adverse effects MH - *Hematopoietic Stem Cell Mobilization/methods MH - *Hematopoietic Stem Cell Transplantation MH - Humans MH - Leukapheresis MH - Retrospective Studies OTO - NOTNLM OT - granulocyte colony-stimulating factor OT - high dose OT - mobilization OT - pediatric patients OT - poor mobilizers EDAT- 2022/06/07 06:00 MHDA- 2022/10/07 06:00 CRDT- 2022/06/06 13:19 PHST- 2022/04/15 00:00 [revised] PHST- 2021/09/24 00:00 [received] PHST- 2022/05/17 00:00 [accepted] PHST- 2022/06/07 06:00 [pubmed] PHST- 2022/10/07 06:00 [medline] PHST- 2022/06/06 13:19 [entrez] AID - 10.1002/jca.21994 [doi] PST - ppublish SO - J Clin Apher. 2022 Oct;37(5):420-429. doi: 10.1002/jca.21994. Epub 2022 Jun 4.