PMID- 35662977
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2398-8835 (Electronic)
IS  - 2398-8835 (Linking)
VI  - 5
IP  - 4
DP  - 2022 Jul
TI  - Assessing the role of eosinophil-mediated immune response markers in detecting 
      hookworm infection: A case-control study in Kintampo, Ghana.
PG  - e674
LID - 10.1002/hsr2.674 [doi]
LID - e674
AB  - BACKGROUND AND AIM: Human hookworm disease caused by Ancylostoma duodenale and 
      Necator americanus is a serious public health problem. Hookworm infection 
      activates eosinophil-mediated tissue inflammatory responses, involving the 
      production of the eosinophil-specific chemokine (eotaxin), recruitment of 
      eosinophils, secretion of the cationic protein, and production of antiparasite 
      immunoglobulin E (IgE). We investigated eosinophil-mediated immune response as 
      markers (CCL11, eosinophil cationic protein [ECP], and IgE) for detecting 
      hookworm infection. METHODS: This case-control study was carried out in hookworm 
      endemic areas within the Kintampo North Municipality.Forty hookworm-positive 
      subjects and 36 apparently healthy individuals were recruited as cases and 
      controls, respectively. Stool samples were collected for hookworm detection by 
      the Kato-Katz technique and speciation by polymerase chain reaction. 
      Approximately, 5 ml of intravenous blood was used to obtain plasma for the 
      immunological assays. RESULTS: Of eosinophil-mediated immune response markers 
      studied, ECP and CCL11 were significantly higher among hookworm patients compared 
      to controls. Increasing CCL11 (beta = -0.81, p = 0.015) was associated with a 
      significant decrease hookworm intensity. However, increasing eosinophil count 
      (beta = 0.62, p = 0.027) was associated with significant increase in hookworm 
      intensity. In receiver operator characteristics analysis, ECP could significantly 
      detect hookworm infection with a very high area under the curve 
      (AUC) (AUC = 0.97, p < 0.0001). At a cutoff of 39.05, ECP was the best 
      eosinophil-mediated immune response marker for detecting hookworm infection with 
      a sensitivity of 97.2%, specificity of 87.8%, a positive predictive value of 
      89.7%, and a negative predictive value of 96.6%. CONCLUSION: ECP best predicts 
      eosinophil-mediated immune response for detecting hookworm infection, while CCL11 
      and eosinophil count better predict the intensity of hookworm. Moreover, the ECP 
      level is a good indicator of hookworm infection and intensity and may require 
      additional investigations to augment current hookworm diagnostic techniques.
CI  - (c) 2022 The Authors. Health Science Reports published by Wiley Periodicals LLC.
FAU - Sakyi, Samuel A
AU  - Sakyi SA
AUID- ORCID: 0000-0001-5168-4762
AD  - Department of Molecular Medicine, School of Medicine and Dentistry Kwame Nkrumah 
      University of Science and Technology Kumasi Ghana.
FAU - Amoani, Benjamin
AU  - Amoani B
AUID- ORCID: 0000-0002-4086-3033
AD  - Department of Biomedical Science, School of Medicine and Dentistry University of 
      Cape Coast Cape Coast Ghana.
FAU - Opoku, Stephen
AU  - Opoku S
AD  - Department of Molecular Medicine, School of Medicine and Dentistry Kwame Nkrumah 
      University of Science and Technology Kumasi Ghana.
FAU - Dzata, Lawrence
AU  - Dzata L
AD  - Department of Microbiology and Immunology, School of Medical Sciences University 
      of Cape Coast Cape Coast Ghana.
FAU - Aniagyei, Wilfred
AU  - Aniagyei W
AUID- ORCID: 0000-0003-4706-0046
AD  - Department of Biomedical Science, School of Medicine and Dentistry University of 
      Cape Coast Cape Coast Ghana.
FAU - Senu, Ebenezer
AU  - Senu E
AUID- ORCID: 0000-0002-2973-8952
AD  - Department of Molecular Medicine, School of Medicine and Dentistry Kwame Nkrumah 
      University of Science and Technology Kumasi Ghana.
FAU - Dankwa, Kwabena
AU  - Dankwa K
AD  - Department of Microbiology and Immunology, School of Medical Sciences University 
      of Cape Coast Cape Coast Ghana.
FAU - Wilson, Michael D
AU  - Wilson MD
AD  - Parasitology Department, Noguchi Memorial Institute for Medical Research, College 
      of Health Sciences University of Ghana Legon Ghana.
LA  - eng
PT  - Journal Article
DEP - 20220603
PL  - United States
TA  - Health Sci Rep
JT  - Health science reports
JID - 101728855
PMC - PMC9165202
OTO - NOTNLM
OT  - eosinophil cationic protein
OT  - eosinophils-mediated immune response
OT  - eotaxin (CCL11)
OT  - hookworm infection
OT  - immunoglobulin E
COIS- The authors declare no conflict of interest.
EDAT- 2022/06/07 06:00
MHDA- 2022/06/07 06:01
PMCR- 2022/06/03
CRDT- 2022/06/06 13:51
PHST- 2022/02/21 00:00 [received]
PHST- 2022/05/22 00:00 [revised]
PHST- 2022/05/23 00:00 [accepted]
PHST- 2022/06/06 13:51 [entrez]
PHST- 2022/06/07 06:00 [pubmed]
PHST- 2022/06/07 06:01 [medline]
PHST- 2022/06/03 00:00 [pmc-release]
AID - HSR2674 [pii]
AID - 10.1002/hsr2.674 [doi]
PST - epublish
SO  - Health Sci Rep. 2022 Jun 3;5(4):e674. doi: 10.1002/hsr2.674. eCollection 2022 
      Jul.