PMID- 35664985
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2690-442X (Electronic)
IS  - 2690-442X (Linking)
VI  - 1
IP  - 2
DP  - 2021 Jun
TI  - An appraisal of laboratory models of androgenetic alopecia: A systematic review.
PG  - e15
LID - 10.1002/ski2.15 [doi]
LID - e15
AB  - BACKGROUND: Androgenetic alopecia (AGA) is the most common form of non-scarring 
      alopecia in humans. Several studies have used different laboratory models to 
      study the pathogenesis and interventions for AGA. These study models have proved 
      beneficial and have led to the approval of two drugs. However, the need to build 
      on existing knowledge remains by examining the relevance of study models to the 
      disease. OBJECTIVE: We sought to appraise laboratory or pre-clinical models of 
      AGA. METHOD: We searched through databases (PubMed, ScienceDirect, Web of 
      Science, World CAT, Scopus and Google Scholar) for articles on AGA-related 
      studies from 1942 to March 2019 with a focus on study models. RESULTS: The search 
      rendered 101 studies after screening and deduplication. Several studies (70) used 
      in vitro models, mostly consisting of two-dimensional monolayer cells for 
      experiments involving the characterization of androgen and 5-alpha reductase 
      (5AR) and inhibition thereof, the effects of dihydrotestosterone (DHT) and 
      biomarker(s) of AGA. Twenty-seven studies used in vivo models of mice and monkeys 
      to investigate DHT synthesis, the expression and inhibition of 5AR and hair 
      growth. Only four studies used AGA-related or healthy excisional/punch biopsy 
      explants as ex vivo models to study the action of 5AR inhibitors and 
      AGA-associated genes. No study used three-dimensional [3-D] organoids or 
      organotypic human skin culture models. CONCLUSION: We recommend clinically 
      relevant laboratory models like human or patient-derived 3-D organoids or 
      organotypic skin in AGA-related studies. These models are closer to human scalp 
      tissue and minimize the use of laboratory animals and could ultimately facilitate 
      novel therapeutics.
CI  - (c) 2021 The Authors. Skin Health and Disease published by John Wiley & Sons Ltd on 
      behalf of British Association of Dermatologists.
FAU - Ntshingila, S
AU  - Ntshingila S
AD  - Hair and Skin Research Laboratory Division of Dermatology Department of Medicine 
      Faculty of Health Sciences and Groote Schuur Hospital University of Cape Town 
      Cape Town South Africa.
FAU - Khumalo, N P
AU  - Khumalo NP
AUID- ORCID: 0000-0002-1686-1900
AD  - Hair and Skin Research Laboratory Division of Dermatology Department of Medicine 
      Faculty of Health Sciences and Groote Schuur Hospital University of Cape Town 
      Cape Town South Africa.
FAU - Engel, M
AU  - Engel M
AD  - Department of Medicine Faculty of Health Sciences and Groote Schuur Hospital 
      University of Cape Town Cape Town South Africa.
FAU - Arowolo, A T
AU  - Arowolo AT
AUID- ORCID: 0000-0002-1402-8835
AD  - Hair and Skin Research Laboratory Division of Dermatology Department of Medicine 
      Faculty of Health Sciences and Groote Schuur Hospital University of Cape Town 
      Cape Town South Africa.
LA  - eng
PT  - Journal Article
DEP - 20210305
PL  - England
TA  - Skin Health Dis
JT  - Skin health and disease
JID - 9918227353706676
PMC - PMC9060143
COIS- The authors have no conflict of interest to declare.
EDAT- 2021/03/05 00:00
MHDA- 2021/03/05 00:01
PMCR- 2021/03/05
CRDT- 2022/06/06 14:22
PHST- 2020/10/19 00:00 [received]
PHST- 2021/01/04 00:00 [revised]
PHST- 2021/01/05 00:00 [accepted]
PHST- 2022/06/06 14:22 [entrez]
PHST- 2021/03/05 00:00 [pubmed]
PHST- 2021/03/05 00:01 [medline]
PHST- 2021/03/05 00:00 [pmc-release]
AID - SKI215 [pii]
AID - 10.1002/ski2.15 [doi]
PST - epublish
SO  - Skin Health Dis. 2021 Mar 5;1(2):e15. doi: 10.1002/ski2.15. eCollection 2021 Jun.