PMID- 3566533
OWN - NLM
STAT- MEDLINE
DCOM- 19870427
LR  - 20131121
IS  - 0098-6127 (Print)
IS  - 0098-6127 (Linking)
VI  - 14
IP  - 2
DP  - 1987
TI  - Changes of euchromatin/heterochromatin ratios in cell nuclei of the aortic 
      adventitia in diabetic rats.
PG  - 66-75
AB  - The main objective of this study was to determine quantitatively 
      euchromatin/heterochromatin (EU/HET) ratios of cell nuclei in the adventitia of 2 
      weeks streptozotocin induced diabetic rats combined with acridine orange (AO) 
      ultracytochemistry. Specimens of the aorta above and below the orificium of the 
      a. renalis were treated with AO and then prepared for electron microscopy. Images 
      of 295 nuclei randomly selected from 180 ultrathin sections were analysed by a 
      computer assisted image analyser method. The findings that surface areas of 
      euchromatin portions and those of total nuclei are dependent variables 
      (correlation coefficients 0.93-0.99), however, the EU/HET ratios and total 
      surface areas are independent variables are essential prerequisites for an 
      efficient application of this method. Determinations of EU/HET ratios revealed 
      that populations of AO labeled cell nuclei consistently have higher ratios 
      (2.17-3.38) than nuclei not labeled by AO (1.26-2.12). On the basis of 2583 
      nuclei counted AO positive cell nuclei were found to increase from 12% in control 
      to 23% in diabetic rats. Changing EU/HET ratios are interpreted in terms of 
      differential RNA synthetic activity enhanced in the early diabetic state. For 
      these cells AO serves as a specific nuclear marker.
FAU - Lehmann, R R
AU  - Lehmann RR
FAU - Nienhaus, R H
AU  - Nienhaus RH
FAU - Denes, R M
AU  - Denes RM
FAU - Steinbach, T
AU  - Steinbach T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Artery
JT  - Artery
JID - 7508494
RN  - 0 (Chromatin)
RN  - 0 (Heterochromatin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Aorta/*ultrastructure
MH  - Chromatin/*ultrastructure
MH  - Diabetes Mellitus, Experimental/*pathology
MH  - Heterochromatin/ultrastructure
MH  - Male
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
PST - ppublish
SO  - Artery. 1987;14(2):66-75.