PMID- 35665345
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2296-858X (Print)
IS  - 2296-858X (Electronic)
IS  - 2296-858X (Linking)
VI  - 9
DP  - 2022
TI  - Efficient Assay and Marker Significance of NAD(+) in Human Blood.
PG  - 886485
LID - 10.3389/fmed.2022.886485 [doi]
LID - 886485
AB  - Oxidized nicotinamide adenine dinucleotide (NAD(+)) is a biological molecule of 
      systemic importance. Essential role of NAD(+) in cellular metabolism relies on 
      the substrate action in various redox reactions and cellular signaling. This work 
      introduces an efficient enzymatic assay of NAD(+) content in human blood using 
      recombinant formate dehydrogenase (FDH, EC 1.2.1.2), and demonstrates its 
      diagnostic potential, comparing NAD(+) content in the whole blood of control 
      subjects and patients with cardiac or neurological pathologies. In the control 
      group (n = 22, 25-70 years old), our quantification of the blood concentration of 
      NAD(+) (18 muM, minimum 15, max 23) corresponds well to NAD(+) quantifications 
      reported in literature. In patients with demyelinating neurological diseases (n = 
      10, 18-55 years old), the NAD(+) levels significantly (p < 0.0001) decrease (to 
      14 muM, min 13, max 16), compared to the control group. In cardiac patients with 
      the heart failure of stage II and III according to the New York Heart Association 
      (NYHA) functional classification (n = 24, 42-83 years old), the blood levels of 
      NAD(+) (13 muM, min 9, max 18) are lower than those in the control subjects (p < 
      0.0001) or neurological patients (p = 0.1). A better discrimination of the 
      cardiac and neurological patients is achieved when the ratios of NAD(+) to the 
      blood creatinine levels, mean corpuscular volume or potassium ions are compared. 
      The proposed NAD(+) assay provides an easy and robust tool for clinical analyses 
      of an important metabolic indicator in the human blood.
CI  - Copyright (c) 2022 Balashova, Zavileyskiy, Artiukhov, Shaposhnikov, Sidorova, 
      Tishkov, Tramonti, Pometun and Bunik.
FAU - Balashova, Natalia V
AU  - Balashova NV
AD  - Department of Clinical Laboratory Diagnostics, Faculty of Advanced Medicine, M.F. 
      Vladimirsky Moscow Regional Research and Clinical Institute (MONIKI), Moscow, 
      Russia.
AD  - Department of Dietetics and Clinical Nutritionology, Faculty of Continuing 
      Medical Education, RUDN Medical Institute, Moscow, Russia.
FAU - Zavileyskiy, Lev G
AU  - Zavileyskiy LG
AD  - Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 
      Moscow, Russia.
FAU - Artiukhov, Artem V
AU  - Artiukhov AV
AD  - Department of Biokinetics, A. N. Belozersky Institute of Physico-Chemical 
      Biology, Lomonosov Moscow State University, Moscow, Russia.
AD  - Department of Biochemistry, Sechenov University, Moscow, Russia.
FAU - Shaposhnikov, Leonid A
AU  - Shaposhnikov LA
AD  - Department of Chemical Enzymology, Faculty of Chemistry, Lomonosov Moscow State 
      University, Moscow, Russia.
FAU - Sidorova, Olga P
AU  - Sidorova OP
AD  - Department of Neurology, Faculty of Advanced Medicine, M.F. Vladimirsky Moscow 
      Regional Research and Clinical Institute (MONIKI), Moscow, Russia.
FAU - Tishkov, Vladimir I
AU  - Tishkov VI
AD  - Department of Chemical Enzymology, Faculty of Chemistry, Lomonosov Moscow State 
      University, Moscow, Russia.
AD  - Bach Institute of Biochemistry, Federal Research Centre "Fundamentals of 
      Biotechnology" of the Russian Academy of Sciences, Moscow, Russia.
FAU - Tramonti, Angela
AU  - Tramonti A
AD  - Institute of Molecular Biology and Pathology, Italian National Research Council, 
      Department of Biochemical Sciences "A. Rossi Fanelli," Sapienza University of 
      Rome, Rome, Italy.
FAU - Pometun, Anastasia A
AU  - Pometun AA
AD  - Department of Chemical Enzymology, Faculty of Chemistry, Lomonosov Moscow State 
      University, Moscow, Russia.
AD  - Bach Institute of Biochemistry, Federal Research Centre "Fundamentals of 
      Biotechnology" of the Russian Academy of Sciences, Moscow, Russia.
FAU - Bunik, Victoria I
AU  - Bunik VI
AD  - Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 
      Moscow, Russia.
AD  - Department of Biokinetics, A. N. Belozersky Institute of Physico-Chemical 
      Biology, Lomonosov Moscow State University, Moscow, Russia.
AD  - Department of Biochemistry, Sechenov University, Moscow, Russia.
LA  - eng
PT  - Journal Article
DEP - 20220519
PL  - Switzerland
TA  - Front Med (Lausanne)
JT  - Frontiers in medicine
JID - 101648047
PMC - PMC9162244
OTO - NOTNLM
OT  - Charcot-Marie-Tooth disease
OT  - NAD+ in human blood
OT  - cardiac patient
OT  - formate dehydrogenase
OT  - metabolic markers
OT  - neurological patient
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/06/07 06:00
MHDA- 2022/06/07 06:01
PMCR- 2022/05/19
CRDT- 2022/06/06 14:28
PHST- 2022/02/28 00:00 [received]
PHST- 2022/04/07 00:00 [accepted]
PHST- 2022/06/06 14:28 [entrez]
PHST- 2022/06/07 06:00 [pubmed]
PHST- 2022/06/07 06:01 [medline]
PHST- 2022/05/19 00:00 [pmc-release]
AID - 10.3389/fmed.2022.886485 [doi]
PST - epublish
SO  - Front Med (Lausanne). 2022 May 19;9:886485. doi: 10.3389/fmed.2022.886485. 
      eCollection 2022.