PMID- 35665791
OWN - NLM
STAT- MEDLINE
DCOM- 20220622
LR  - 20240109
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 13
IP  - 12
DP  - 2022 Jun 20
TI  - beta(2-->1) chicory and beta(2-->1)-beta(2-->6) agave fructans protect the human intestinal 
      barrier function in vitro in a stressor-dependent fashion.
PG  - 6737-6748
LID - 10.1039/d2fo00534d [doi]
AB  - Dietary fibers such as fructans can protect the intestinal epithelial barrier 
      integrity, but the mechanisms underlying this protection are not completely 
      understood. We aimed to study the protective effect of beta(2-->1)-beta(2-->6) branched 
      graminan-type fructans (GTFs) on gut epithelial barrier function that was 
      disrupted by three different agents which impact the barrier function via 
      different cellular mechanisms. The effects of GTFs were compared with those of 
      linear beta(2-->1) inulin-type fructans (ITFs). T84 intestinal epithelial monolayers 
      were incubated with GTFs and ITFs. Afterwards, the monolayers were challenged 
      with the barrier disruptors calcium ionophore A23187, 12-myristate 13-acetate 
      (PMA) and deoxynivalenol (DON). Transepithelial resistance was measured with an 
      electric cell-substrate impedance sensing system. All fructans studied prevented 
      the barrier disruption induced by A23187. ITF II protected from the disruptive 
      effects of PMA. However, none of the studied fructans influenced the disruption 
      induced by DON. As a measure of disruption-induced inflammation, interleukin-8 
      (IL-8) production by the intestinal epithelium was determined by ELISA. The 
      production of IL-8 induced by A23187 was decreased by all fructans, whereas IL-8 
      production induced by DON decreased only upon pre-treatment with ITF II. None of 
      the studied fructans prevented PMA induced IL-8 production. GTFs just like ITFs 
      can influence the barrier function and inflammatory processes in gut epithelial 
      cells in a structure-dependent fashion. These distinct protective effects are 
      dependent on the different signaling pathways that lead to gut barrier 
      disruption.
FAU - Fernandez-Lainez, Cynthia
AU  - Fernandez-Lainez C
AUID- ORCID: 0000-0001-7468-3420
AD  - Immunoendocrinology, Division of Medical Biology, Department of Pathology and 
      Medical Biology, University Medical Centre Groningen, Hanzeplein 1, 9713 GZ, 
      Groningen, The Netherlands. c.fernandez.lainez@umcg.nl.
AD  - Laboratorio de Errores innatos del Metabolismo y Tamiz, Instituto Nacional de 
      Pediatria, Av. Iman 1, 04530, Ciudad de Mexico, Mexico.
AD  - Posgrado en Ciencias Biologicas, Universidad Nacional Autonoma de Mexico. 
      Edificio D, 1 degrees  Piso. Circuito de Posgrados, Ciudad Universitaria, 04510, Ciudad 
      de Mexico, Mexico.
FAU - Logtenberg, Madelon J
AU  - Logtenberg MJ
AD  - Laboratory of Food Chemistry, Wageningen University, Bornse Weilanden 9, 6708 WG, 
      Wageningen, The Netherlands. madelon.logtenberg@wur.nl.
FAU - Tang, Xin
AU  - Tang X
AD  - Immunoendocrinology, Division of Medical Biology, Department of Pathology and 
      Medical Biology, University Medical Centre Groningen, Hanzeplein 1, 9713 GZ, 
      Groningen, The Netherlands. c.fernandez.lainez@umcg.nl.
FAU - Schols, Henk A
AU  - Schols HA
AD  - Laboratory of Food Chemistry, Wageningen University, Bornse Weilanden 9, 6708 WG, 
      Wageningen, The Netherlands. madelon.logtenberg@wur.nl.
FAU - Lopez-Velazquez, Gabriel
AU  - Lopez-Velazquez G
AD  - Laboratorio de Biomoleculas y Salud Infantil, Instituto Nacional de Pediatria, 
      Av. Iman 1, 04530, Cuidad de Mexico, Mexico. glv_1999@ciencias.unam.mx.
FAU - de Vos, Paul
AU  - de Vos P
AD  - Immunoendocrinology, Division of Medical Biology, Department of Pathology and 
      Medical Biology, University Medical Centre Groningen, Hanzeplein 1, 9713 GZ, 
      Groningen, The Netherlands. c.fernandez.lainez@umcg.nl.
LA  - eng
PT  - Journal Article
DEP - 20220620
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Fructans)
RN  - 0 (Interleukin-8)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 9005-80-5 (Inulin)
SB  - IM
MH  - *Agave/metabolism
MH  - Calcimycin/pharmacology
MH  - *Cichorium intybus/metabolism
MH  - Fructans/pharmacology
MH  - Humans
MH  - Interleukin-8/genetics/metabolism
MH  - Intestinal Mucosa/metabolism
MH  - Inulin/metabolism/pharmacology
EDAT- 2022/06/07 06:00
MHDA- 2022/06/23 06:00
CRDT- 2022/06/06 14:59
PHST- 2022/06/07 06:00 [pubmed]
PHST- 2022/06/23 06:00 [medline]
PHST- 2022/06/06 14:59 [entrez]
AID - 10.1039/d2fo00534d [doi]
PST - epublish
SO  - Food Funct. 2022 Jun 20;13(12):6737-6748. doi: 10.1039/d2fo00534d.