PMID- 35669002
OWN - NLM
STAT- MEDLINE
DCOM- 20220608
LR  - 20231101
IS  - 1178-2013 (Electronic)
IS  - 1176-9114 (Print)
IS  - 1176-9114 (Linking)
VI  - 17
DP  - 2022
TI  - Self-Assembled Nano-Peptide Hydrogels with Human Umbilical Cord Mesenchymal Stem 
      Cell Spheroids Accelerate Diabetic Skin Wound Healing by Inhibiting Inflammation 
      and Promoting Angiogenesis.
PG  - 2459-2474
LID - 10.2147/IJN.S363777 [doi]
AB  - BACKGROUND: Non-healing skin wounds are a common complication in diabetic 
      patients. Vector biomaterials embedded with mesenchymal stem cells (MSCs) are 
      considered a promising treatment approach. In this study, we presented a novel 
      and effective approach to accelerate diabetic skin wound healing. METHODS AND 
      MATERIALS: Human umbilical cord mesenchymal stem cells (hUC-MSCs) were shaped 
      into spheres. RADA16-I, KLT, and RGD nanopeptides were selected for self-assembly 
      into hydrogels. hUC-MSCs spheroids (hUC-MSCsp) were combined in vitro with 
      self-assembled nanopeptide hydrogels and subsequently transplanted into a mouse 
      model of diabetic skin trauma. RESULTS: Compared with the PBS, hUC-MSCs, 
      hUC-MSCsp, and hUC-MSCs with hydrogel groups, hUC-MSCsp with hydrogel 
      significantly accelerated wound healing (p<0.01) and shortened the healing time 
      (10 vs 14 vs 21 days). The expressions of IL-6, IL-10, IL-1beta, and TNF-alpha were 
      significantly decreased (p<0.001). The expression of VEGF was significantly 
      higher in the hUC-MSCsp with hydrogel group (p<0.05), and the density of 
      neovascularization in the fresh skin tissue at the wound was also remarkably 
      increased (p<0.01). CONCLUSION: Nanopeptide hydrogels loaded with hUC-MSCsp 
      accelerated diabetic skin wound healing by inhibiting inflammation and promoting 
      angiogenesis compared with conventional stem cell transplantation, which deserves 
      further investigation.
CI  - (c) 2022 Xue et al.
FAU - Xue, Junshuai
AU  - Xue J
AD  - Department of General Surgery, Qilu Hospital of Shandong University, Jinan City, 
      Shandong Province, People's Republic of China.
FAU - Sun, Nianfeng
AU  - Sun N
AUID- ORCID: 0000-0002-1997-9832
AD  - Women's and Children's Hospital Affiliated to Qingdao University, Qingdao, 
      266001, People's Republic of China.
FAU - Liu, Yang
AU  - Liu Y
AUID- ORCID: 0000-0002-4266-8282
AD  - Department of General Surgery, Vascular Surgery, Qilu Hospital of Shandong 
      University, Jinan City, Shandong Province, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20220530
PL  - New Zealand
TA  - Int J Nanomedicine
JT  - International journal of nanomedicine
JID - 101263847
RN  - 0 (Hydrogels)
SB  - IM
EIN - Int J Nanomedicine. 2022 Jul 11;17:3057-3058. PMID: 35844972
MH  - Animals
MH  - *Diabetes Mellitus/metabolism
MH  - Humans
MH  - Hydrogels/pharmacology
MH  - Inflammation/metabolism
MH  - *Mesenchymal Stem Cell Transplantation/methods
MH  - *Mesenchymal Stem Cells
MH  - Mice
MH  - Umbilical Cord
MH  - Wound Healing
PMC - PMC9166320
OTO - NOTNLM
OT  - diabetic wound
OT  - human umbilical cord mesenchymal stem cell
OT  - nanopeptide hydrogels
OT  - spheroid
OT  - treatment
COIS- The authors declare that they have no competing interests.
EDAT- 2022/06/08 06:00
MHDA- 2022/06/09 06:00
PMCR- 2022/05/30
CRDT- 2022/06/07 02:22
PHST- 2022/02/23 00:00 [received]
PHST- 2022/05/23 00:00 [accepted]
PHST- 2022/06/07 02:22 [entrez]
PHST- 2022/06/08 06:00 [pubmed]
PHST- 2022/06/09 06:00 [medline]
PHST- 2022/05/30 00:00 [pmc-release]
AID - 363777 [pii]
AID - 10.2147/IJN.S363777 [doi]
PST - epublish
SO  - Int J Nanomedicine. 2022 May 30;17:2459-2474. doi: 10.2147/IJN.S363777. 
      eCollection 2022.